Construct: ORF TRCN0000488689
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019922.1_s317c1
- DNA Barcode:
- GGTGCGCGACCTCAATGTCACGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K3 (5606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488689
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_001316332.2 | 99.7% | 99.6% | 273T>C;954_955insG |
| 2 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_002756.4 | 99.7% | 99.6% | 273T>C;954_955insG |
| 3 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_005256723.2 | 99.7% | 99.6% | 273T>C;954_955insG |
| 4 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_011523959.2 | 99.7% | 99.6% | 273T>C;954_955insG |
| 5 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024858.1 | 99.7% | 99.6% | 273T>C;954_955insG |
| 6 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024859.1 | 99.7% | 99.6% | 273T>C;954_955insG |
| 7 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024857.2 | 92.5% | 92.4% | 1_75del;348T>C;1029_1030insG |
| 8 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_145109.3 | 91.4% | 91.3% | 1_87del;360T>C;1041_1042insG |
| 9 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_011523958.2 | 78.6% | 71.5% | 273T>C;685_686ins140;815_894delinsG |
| 10 | mouse | 26397 | Map2k3 | mitogen-activated protein k... | NM_008928.4 | 83.3% | 89% | (many diffs) |
| 11 | mouse | 26397 | Map2k3 | mitogen-activated protein k... | XM_006533368.3 | 73.7% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1026
- ORF length:
- 957
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtccaagcca cccgcaccca accccacacc cccccggaac ctggactccc 121 ggaccttcat caccattgga gacagaaact ttgaggtgga ggctgatgac ttggtgacca 181 tctcagaact gggccgtgga gcctatgggg tggtagagaa ggtgcggcac gcccagagcg 241 gcaccatcat ggccgtgaag cggatccggg ccaccgtgaa ctcacaggag cagaagcggc 301 tgctcatgga cctggacatc aacatgcgca cggtcgactg cttctacact gtcaccttct 361 acggggcact attcagagag ggagacgtgt ggatctgcat ggagctcatg gacacatcct 421 tggacaagtt ctaccggaag gtgctggata aaaacatgac aattccagag gacatccttg 481 gggagattgc tgtgtctatc gtgcgggccc tggagcatct gcacagcaag ctgtcggtga 541 tccacagaga tgtgaagccc tccaatgtcc ttatcaacaa ggagggccat gtgaagatgt 601 gtgactttgg catcagtggc tacttggtgg actctgtggc caagacgatg gatgccggct 661 gcaagcccta catggccccT GAGAGGATCA ACCCAGAGCT GAACCAGAAG GGCTACAATG 721 TCAAGTCCGA CGTCTGGAGC CTGGGCATCA CCATGATTGA GATGGCCATC CTGCGGTTCC 781 CTTACGAGTC CTGGGGGACC CCGTTCCAGC AGCTGAAGCA GGTGGTGGAG GAGCCGTCCC 841 CCCAGCTCCC AGCCGACCGT TTCTCCCCCG AGTTTGTGGA CTTCACTGCT CAGTGCCTGA 901 GGAAGAACCC CGCAGAGCGT ATGAGCTACC TGGAGCTGAT GGAGCACCCC TTCTTCACCT 961 TGCACAAAAC CAAGAAGACG GACATTGCTG CCTTCGTGAA GGAGATCCTG GGAGAAGACT 1021 CAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGAGG TGCGCGACCT CAATGTCACG TGACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt