Construct: ORF TRCN0000488693
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022028.1_s317c1
- DNA Barcode:
- GCCGGCGAGTATAGGGCATCAAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC28A2 (9153)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488693
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9153 | SLC28A2 | solute carrier family 28 me... | NM_004212.4 | 99.9% | 100% | 282G>A |
| 2 | human | 9153 | SLC28A2 | solute carrier family 28 me... | XM_011522198.2 | 99.9% | 100% | 282G>A |
| 3 | human | 9153 | SLC28A2 | solute carrier family 28 me... | XM_011522199.1 | 94.9% | 94.8% | 282G>A;1648_1649ins99 |
| 4 | human | 9153 | SLC28A2 | solute carrier family 28 me... | XM_011522200.2 | 85.3% | 80.2% | 282G>A;1566_1567ins181;1686_1687ins107 |
| 5 | human | 9153 | SLC28A2 | solute carrier family 28 me... | XM_011522201.2 | 83.7% | 83.4% | (many diffs) |
| 6 | human | 9153 | SLC28A2 | solute carrier family 28 me... | XM_011522202.2 | 55.1% | 55.1% | 0_1ins885 |
| 7 | mouse | 269346 | Slc28a2 | solute carrier family 28 (s... | XM_006499602.3 | 73.9% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2043
- ORF length:
- 1974
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagaaagca agtggaagac agtccattgc tctgtccaca gtggagactg 121 gcacagtgaa cccggggctg gagctcatgg aaaaagaagt agagcctgag ggaagcaaga 181 ggactgacgc acaaggacac agcctggggg atggactggg cccttccact taccagagga 241 ggagtcggtg gcctttcagc aaagcaagaa gtttctgcaa aacacacgcc agcttgttca 301 agaagatcct gttgggcctg ttgtgtttgg cctatgctgc ctatctccta gcagcttgca 361 tcttgaattt ccagagggca ctggccttgt ttgtcatcac ctgcttggtg atctttgtcc 421 tggttcactc gtttttgaaa aagctcctgg gcaaaaaatt aacaagatgt ctgaagccct 481 ttgaaaactc ccgcctgagg ctttggacga aatgggtgtt tgcaggagtc tccttggttg 541 gccttatact gtggttggct ttagacacag cccaaaggcc agagcagctg atcccctttg 601 caggaatctg catgttcatc cttatcctct ttgcctgctc caaacaccac agcgcagtgt 661 cctggaggac agtgttttcg ggcctaggtc ttcaatttgt ctttgggatc ttggtcatca 721 gaactgatct tggatatact gtatttcagt ggctgggaga gcaggtccag attttcctga 781 actacactgt ggccggctcc agttttgtct ttggggatac actggtcaag gatgtctttg 841 cttttcaggc cttaccaatc atcattttct ttggatgtgt ggtgtccatt ctctactacc 901 tgggccttgt gcaatgggta gttcagaagg tcgcctggtt tttacaaatc actatgggca 961 ccactgctac agagaccctg gctgtggcag gaaacatctt tgtgggtatg acagaggcac 1021 ctctgctcat ccgtccctac cttggggaca tgacactctc tgaaatccat gcggtgatga 1081 ctggagggtt tgccaccatt tctggcactg tgctgggagc cttcatagcc tttggggttg 1141 atgcatcatc cctgatttct gcctctgtga tggccgcccc ttgtgctctc gcctcatcaa 1201 agctagcgta tccggaagtg gaggagtcca agttcaagag tgaggagggg gtaaagctgc 1261 cccgtgggaa ggagaggaat gtcctggaag ctgccagcaa cggagccgta gatgccatag 1321 gccttgctac taatgtagca gccaacctga ttgccttttt ggctgtgttg gccttcatca 1381 atgctgccct ctcctggctg ggggaattgg tggacataca ggggctcact ttccaggtca 1441 tctgctccta tctcctaagg cccatggttt tcatgatggg tgtagagtgg acagactgtc 1501 caatggtggc tgagatggtg ggaatcaagt tcttcataaa tgagtttgtg gcttatcagc 1561 aactgtctca atacaagaac aaacgtctct ctggaatgga ggagtggatt gagggagaga 1621 aacagtggat ttctgtgaga gctgaaatca ttacaacatt ttcactctgt ggatttgcca 1681 atcttagttc cataggaatc acacttGGAG GCTTGACATC AATAGTACCT CACCGGAAGA 1741 GTGACTTGTC CAAGGTTGTG GTCAGGGCCC TCTTCACAGG GGCCTGTGTA TCCCTTATCA 1801 GTGCCTGTAT GGCAGGAATC CTCTATGTCC CCAGGGGAGC TGAAGCTGAC TGTGTCTCCT 1861 TCCCAAACAC AAGTTTCACC AATAGAACCT ATGAGACCTA CATGTGCTGC AGAGGGCTCT 1921 TTCAGAGTAC TTCTCTGAAT GGCACCAACC CTCCTTCTTT TTCTGGTCCC TGGGAAGATA 1981 AGGAGTTCAG TGCTATGGCC CTTACTAACT GCTGTGGATT CTACAACAAT ACCGTCTGTG 2041 CCTAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2101 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2161 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCGGCGAG TATAGGGCAT CAAAGACGCG 2221 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt