Construct: ORF TRCN0000488695

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021919.1_s317c1
DNA Barcode:
GTGAATATTCCGCACACTGGACCA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ADCYAP1 (116)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488695
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 116 ADCYAP1 adenylate cyclase activatin... NM_001099733.2 99.8% 100% 126G>A
2 human 116 ADCYAP1 adenylate cyclase activatin... NM_001117.5 99.8% 100% 126G>A
3 human 116 ADCYAP1 adenylate cyclase activatin... XM_005258081.4 55.7% 55.8% 1_417del;543G>A
4 mouse 11516 Adcyap1 adenylate cyclase activatin... NM_001315503.1 85% 82.3% (many diffs)
5 mouse 11516 Adcyap1 adenylate cyclase activatin... NM_009625.3 85% 82.3% (many diffs)
6 mouse 11516 Adcyap1 adenylate cyclase activatin... XM_006523498.3 78.1% 74.3% (many diffs)
7 mouse 11516 Adcyap1 adenylate cyclase activatin... XM_006523499.3 64.3% 58.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
600
ORF length:
528
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaccatg tgtagcggag cgaggctggc cctgctggtc tatgggataa
121tcatgcacag cagcgtctac agctcacctg ccgccgccgg actccggttc cccgggatca
181ggccagagga agaggcatac ggcgaggacg gaaacccgct gccagacttc gatggctcgg
241agccgccggg cgcagggagc cccgccTCCG CGCCGCGCGC CGCCGCCGCC TGGTACCGCC
301CGGCCGGGAG AAGAGATGTC GCCCACGGGA TCCTTAACGA GGCCTACCGC AAAGTGCTGG
361ACCAGCTGTC CGCCGGGAAG CACCTGCAGT CGCTCGTGGC CCGGGGCGTG GGTGGGAGCC
421TCGGCGGCGG CGCGGGGGAC GACGCGGAGC CGCTCTCCAA GCGCCACTCG GACGGGATCT
481TCACGGACAG CTACAGCCGC TACCGGAAAC AAATGGCTGT CAAGAAATAC TTGGCGGCCG
541TCCTAGGGAA GAGGTATAAA CAAAGGGTTA AAAACAAAGG ACGCCGAATA GCTTATTTGT
601AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
661TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
721GGCTTTATAT ATCTTGTGGA AAGGACGAGT GAATATTCCG CACACTGGAC CAACGCGTTA
781AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)