Construct: ORF TRCN0000488695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021919.1_s317c1
- DNA Barcode:
- GTGAATATTCCGCACACTGGACCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADCYAP1 (116)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488695
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116 | ADCYAP1 | adenylate cyclase activatin... | NM_001099733.2 | 99.8% | 100% | 126G>A |
| 2 | human | 116 | ADCYAP1 | adenylate cyclase activatin... | NM_001117.5 | 99.8% | 100% | 126G>A |
| 3 | human | 116 | ADCYAP1 | adenylate cyclase activatin... | XM_005258081.4 | 55.7% | 55.8% | 1_417del;543G>A |
| 4 | mouse | 11516 | Adcyap1 | adenylate cyclase activatin... | NM_001315503.1 | 85% | 82.3% | (many diffs) |
| 5 | mouse | 11516 | Adcyap1 | adenylate cyclase activatin... | NM_009625.3 | 85% | 82.3% | (many diffs) |
| 6 | mouse | 11516 | Adcyap1 | adenylate cyclase activatin... | XM_006523498.3 | 78.1% | 74.3% | (many diffs) |
| 7 | mouse | 11516 | Adcyap1 | adenylate cyclase activatin... | XM_006523499.3 | 64.3% | 58.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 600
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaccatg tgtagcggag cgaggctggc cctgctggtc tatgggataa 121 tcatgcacag cagcgtctac agctcacctg ccgccgccgg actccggttc cccgggatca 181 ggccagagga agaggcatac ggcgaggacg gaaacccgct gccagacttc gatggctcgg 241 agccgccggg cgcagggagc cccgccTCCG CGCCGCGCGC CGCCGCCGCC TGGTACCGCC 301 CGGCCGGGAG AAGAGATGTC GCCCACGGGA TCCTTAACGA GGCCTACCGC AAAGTGCTGG 361 ACCAGCTGTC CGCCGGGAAG CACCTGCAGT CGCTCGTGGC CCGGGGCGTG GGTGGGAGCC 421 TCGGCGGCGG CGCGGGGGAC GACGCGGAGC CGCTCTCCAA GCGCCACTCG GACGGGATCT 481 TCACGGACAG CTACAGCCGC TACCGGAAAC AAATGGCTGT CAAGAAATAC TTGGCGGCCG 541 TCCTAGGGAA GAGGTATAAA CAAAGGGTTA AAAACAAAGG ACGCCGAATA GCTTATTTGT 601 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 661 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 721 GGCTTTATAT ATCTTGTGGA AAGGACGAGT GAATATTCCG CACACTGGAC CAACGCGTTA 781 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt