Construct: ORF TRCN0000488706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021086.1_s317c1
- DNA Barcode:
- CTTAATAAGTCGAAATCCGAGGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR139 (124274)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488706
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124274 | GPR139 | G protein-coupled receptor 139 | NM_001002911.3 | 99.9% | 100% | 789A>G |
| 2 | human | 124274 | GPR139 | G protein-coupled receptor 139 | NM_001318483.1 | 73.5% | 73.6% | 0_1ins279;510A>G |
| 3 | mouse | 209776 | Gpr139 | G protein-coupled receptor 139 | NM_001024138.1 | 88% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1131
- ORF length:
- 1059
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagcac acgcacgccc acctcgcagc caacagctcg ctgtcttggt 121 ggtcccccgg ctcggcctgc ggcttgggtt tcgtgcccgt ggtctactac agcctcttgc 181 tgtgcctcgg tttaccagca aatatcttga cagtgatcat cctctcccag ctggtggcaa 241 gaagacagaa gtcctcctac aactatctct tggcactcgc tgctgccgac atcttggtcc 301 tctttttcat agtgtttgtg gacttcctgt tggaagattt catcttgaac atgcagatgc 361 ctcaggtccc cgacaagatc atagaagtgc tggaattctc atccatccac acctccatat 421 ggattactgt accgttaacc attgacaggt atatcgctgt ctgccacccg ctcaagtacc 481 acacggtctc atacccagcc cgcacccgga aagtcattgt aagtgtttac atcacctgct 541 tcctgaccag catcccctat tactggtggc ccaacatctg gactgaagac tacatcagca 601 cctctgtgca tcacgtcctc atctggatcc actgcttcac cgtctacctg gtgccctgct 661 ccatcttctt catcttgaac tcaatcattg tgtacaagct caggaggaag agcaattttc 721 gtctccgtgg ctactccacg gggaagacca ccgccatctt gttcaccatt acctccatct 781 ttgccacact ttGGGCCCCC CGCATCATCA TGATTCTTTA CCACCTCTAT GGGGCGCCCA 841 TCCAGAACCG CTGGCTGGTG CACATCATGT CCGACATTGC CAACATGCTA GCCCTTCTGA 901 ACACAGCCAT CAACTTCTTC CTCTACTGCT TCATCAGCAA GCGGTTCCGC ACCATGGCAG 961 CCGCCACGCT CAAGGCTTTC TTCAAGTGCC AGAAGCAACC TGTACAGTTC TACACCAATC 1021 ATAACTTTTC CATAACAAGT AGCCCCTGGA TCTCGCCGGC AAACTCACAC TGCATCAAGA 1081 TGCTGGTGTA CCAGTATGAC AAAAATGGAA AACCTATAAA AGTATCCCCG TGAGACCCAG 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAC TTAATAAGTC GAAATCCGAG GACACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att