Construct: ORF TRCN0000488717
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020389.1_s317c1
- DNA Barcode:
- AAGCACACCTCCCTACCAGTGAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ILK (3611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488717
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3611 | ILK | integrin linked kinase | NM_001014794.3 | 99.8% | 100% | 297C>T;819G>A |
| 2 | human | 3611 | ILK | integrin linked kinase | NM_001014795.3 | 99.8% | 100% | 297C>T;819G>A |
| 3 | human | 3611 | ILK | integrin linked kinase | NM_004517.4 | 99.8% | 100% | 297C>T;819G>A |
| 4 | human | 3611 | ILK | integrin linked kinase | XM_005252904.5 | 99.8% | 100% | 297C>T;819G>A |
| 5 | human | 3611 | ILK | integrin linked kinase | XM_011520065.1 | 99.8% | 100% | 297C>T;819G>A |
| 6 | human | 3611 | ILK | integrin linked kinase | XM_024448494.1 | 93.4% | 93.5% | 297C>T;447_539del;912G>A |
| 7 | human | 3611 | ILK | integrin linked kinase | XM_024448495.1 | 93.4% | 93.5% | 297C>T;447_539del;912G>A |
| 8 | human | 3611 | ILK | integrin linked kinase | XM_024448496.1 | 93.4% | 93.5% | 297C>T;447_539del;912G>A |
| 9 | human | 3611 | ILK | integrin linked kinase | XM_024448497.1 | 93.4% | 93.5% | 297C>T;447_539del;912G>A |
| 10 | human | 3611 | ILK | integrin linked kinase | XM_017017672.1 | 88.5% | 88.7% | 0_1ins153;144C>T;666G>A |
| 11 | human | 3611 | ILK | integrin linked kinase | XM_024448498.1 | 88.5% | 88.7% | 0_1ins153;144C>T;666G>A |
| 12 | human | 3611 | ILK | integrin linked kinase | XM_024448499.1 | 88.5% | 88.7% | 0_1ins153;144C>T;666G>A |
| 13 | human | 3611 | ILK | integrin linked kinase | NM_001278441.2 | 86.3% | 82.7% | (many diffs) |
| 14 | human | 3611 | ILK | integrin linked kinase | NM_001278442.1 | 70.2% | 70.3% | 0_1ins402;417G>A |
| 15 | human | 3611 | ILK | integrin linked kinase | XM_005252905.3 | 70.2% | 70.3% | 0_1ins402;417G>A |
| 16 | human | 3611 | ILK | integrin linked kinase | XM_024448500.1 | 65.7% | 65.8% | 0_1ins402;45_137del;510G>A |
| 17 | mouse | 16202 | Ilk | integrin linked kinase | NM_001161724.1 | 91.8% | 99.5% | (many diffs) |
| 18 | mouse | 16202 | Ilk | integrin linked kinase | NM_010562.2 | 91.8% | 99.5% | (many diffs) |
| 19 | mouse | 16202 | Ilk | integrin linked kinase | XM_006507389.3 | 85.7% | 92.9% | (many diffs) |
| 20 | mouse | 16202 | Ilk | integrin linked kinase | XM_006507390.3 | 85.7% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1428
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacgac attttcactc agtgccggga gggcaacgca gtcgccgttc 121 gcctgtggct ggacaacacg gagaacgacc tcaaccaggg ggacgatcat ggcttctccc 181 ccttgcactg ggcctgccga gagggccgct ctgctgtggt tgagatgttg atcatgcggg 241 gggcacggat caatgtaatg aaccgtgggg atgacacccc cctgcatctg gcagccagtc 301 atggacaccg tgatattgta cagaagctat tgcagtacaa ggcagacatc aatgcagtga 361 atgaacatgg gaatgtgccc ctgcactatg cctgtttttg gggccaagat caagtggcag 421 aggacctggt ggcaaatggg gcccttgtca gcatctgtaa caagtatgga gagatgcctg 481 tggacaaagc caaggcaccc ctgagagagc ttctccgaga gcgggcagag aagatgggcc 541 agaatctcaa ccgtattcca tacaaggaca cattctggaa ggggaccacc cgcactcggc 601 cccgaaatgg aaccctgaac aaacactctg gcattgactt caaacagctt aacttcctga 661 cgaagctcaa cgagaatcac tctggagagc tatggaaggg ccgctggcag ggcaatgaca 721 ttgtcgtgaa ggtgctgaag gttcgagact ggagtacaag gaagagcagg gacttcaatg 781 aagagtgtcc ccggctcagg attttctcgc atccaaatgt gctcccagtg ctaggtgcct 841 gccagtctcc acctgctcct catcctactc tcatcacaca ctggatgcca tatggatccc 901 tctacaatgt actacatgaa ggcaccaatt tcgtcgtgga ccagagccag gctgtgaagt 961 ttgctttgga catggcaagg ggcatggcct tcctacacac actagagccc ctcatcccac 1021 gacatgcact caatagccGT AGTGTAATGA TTGATGAGGA CATGACTGCC CGAATTAGCA 1081 TGGCTGATGT CAAGTTCTCT TTCCAATGTC CTGGTCGCAT GTATGCACCT GCCTGGGTAG 1141 CCCCCGAAGC TCTGCAGAAG AAGCCTGAAG ACACAAACAG ACGCTCAGCA GACATGTGGA 1201 GTTTTGCAGT GCTTCTGTGG GAACTGGTGA CACGGGAGGT ACCCTTTGCT GACCTCTCCA 1261 ATATGGAGAT TGGAATGAAG GTGGCATTGG AAGGCCTTCG GCCTACCATC CCACCAGGTA 1321 TTTCCCCTCA TGTGTGTAAG CTCATGAAGA TCTGCATGAA TGAAGACCCT GCAAAGCGAC 1381 CCAAATTTGA CATGATTGTG CCTATCCTTG AGAAGATGCA GGACAAGTAG GACCCAGCTT 1441 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1501 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1561 CTTGTGGAAA GGACGAAAGC ACACCTCCCT ACCAGTGAGA ACGCGTTAAG TCgacaatca 1621 acctctggat tacaaaattt gtgaaagatt