Construct: ORF TRCN0000488736
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021464.1_s317c1
- DNA Barcode:
- TGCGTGTCCTTAACCATCACACGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GALNT1 (2589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488736
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2589 | GALNT1 | polypeptide N-acetylgalacto... | NM_020474.3 | 100% | 100% | |
| 2 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | NM_001160404.1 | 93.6% | 98.3% | (many diffs) |
| 3 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | NM_013814.3 | 93.6% | 98.3% | (many diffs) |
| 4 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | XM_006525650.2 | 93.6% | 98.3% | (many diffs) |
| 5 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | XM_017317833.1 | 62.8% | 66.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 78
- ORF end:
- 1755
- ORF length:
- 1677
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcca attgaccatg agaaaatttg catactgcaa ggtggtccta gccacctcct 121 tgatttgggt actcttggat atgttcctgc tgctttactt cagtgaatgc aacaaatgtg 181 atgaaaaaaa ggagagagga cttcctgctg gagatgttct agagccagta caaaagcctc 241 atgaaggtcc tggagaaatg gggaaaccag tcgtcattcc taaagaggat caagaaaaga 301 tgaaagagat gtttaaaatc aatcagttca atttaatggc aagtgagatg attgcactca 361 acagatcttt accagatgtt aggttagaag ggtgtaaaac aaaggtgtat ccagataatc 421 ttcctacaac aagtgtggtg attgttttcc acaatgaggc ttggagcaca cttctgcgaa 481 ctgtccatag tgtcattaat cgctcaccaa gacacatgat agaagaaatt gttctagtag 541 atgatgccag tgaaagagac tttttgaaaa ggcctttaga gagttatgtg aaaaaactaa 601 aagtaccagt tcatgtaatt cgaatggaac aacgttctgg attgatcaga gctagattaa 661 aaggagctgc tgtgtctaaa ggccaagtga tcaccttcct ggatgcccat tgtgagtgta 721 cagtgggatg gctggagcct ctcttggcca ggatcaaaca tgacaggaga acagtggtgt 781 gtcccatcat cgatgtgatc agtgatgata cttttgagta catggcaggc tctgatatga 841 cctatggtgg gttcaactgg aagctcaatt ttcgctggta tcctgttccc caaagagaaa 901 tggacagaag gaaaggtgat cggactcttc ctgtcaggac acctaccatg gcaggaggcc 961 ttttttcaat agacagagat tactttcagg aaattggaac atatgatgct ggaatggata 1021 tttggggagg agaaaaccta gaaatttcct ttaggatttg gcagtgtgga ggaactttgg 1081 aaattgttac atgctcacat gttggacatg tgtttcggaa agctacacct tacacgtttc 1141 caggaggcac agggcagatt atcaataaaa ataacagacg acttgcagaa gtgtggatgg 1201 atgaattcaa gaatttcttc tatataattt ctccaggtgt tacaaaggta gattatggag 1261 atatatcgtc aagagttggt ctaagacaca aactacaatg caaacctttt tcctggtacc 1321 tagagaatat atatcctgat tctcaaattc cacgtcacta tttctcattg ggagagatac 1381 gaaatgtgga aacgaatcag tgtctagata acatGGCTAG AAAAGAGAAT GAAAAAGTTG 1441 GAATTTTTAA TTGCCATGGT ATGGGGGGTA ATCAGGTTTT CTCTTATACT GCCAACAAAG 1501 AAATTAGAAC AGATGACCTT TGCTTGGATG TTTCCAAACT TAATGGCCCA GTTACAATGC 1561 TCAAATGCCA CCACCTAAAA GGCAACCAAC TCTGGGAGTA TGACCCAGTG AAATTAACCC 1621 TGCAGCATGT GAACAGTAAT CAGTGCCTGG ATAAAGCCAC AGAAGAGGAT AGCCAGGTGC 1681 CCAGCATTAG AGACTGCAAT GGAAGTCGGT CCCAGCAGTG GCTTCTTCGA AACGTCACCC 1741 TGCCAGAAAT ATTCTGAAAG CTTGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1801 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1861 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GCGTGTCCTT 1921 AACCATCACA CGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1981 att