Construct: ORF TRCN0000488749
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020383.1_s317c1
- DNA Barcode:
- ATAAGTAATATTGATTAAGTAGCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP4K2 (5871)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488749
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5871 | MAP4K2 | mitogen-activated protein k... | NM_001307990.2 | 99.9% | 100% | 1794C>T |
| 2 | human | 5871 | MAP4K2 | mitogen-activated protein k... | NM_004579.5 | 98.9% | 99% | 1274_1297del;1818C>T |
| 3 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_017018093.2 | 94.5% | 94.5% | 529_530ins132;1662C>T |
| 4 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448629.1 | 94.3% | 94.4% | 1167_1286del;1394_1417del;1938C>T |
| 5 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_011545204.3 | 93.6% | 93.6% | 529_530ins132;1142_1165del;1686C>T |
| 6 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448630.1 | 91.7% | 91.6% | (many diffs) |
| 7 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448631.1 | 89.2% | 89.3% | (many diffs) |
| 8 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_017018095.2 | 74.5% | 74.5% | (many diffs) |
| 9 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448633.1 | 73.7% | 73.8% | (many diffs) |
| 10 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448634.1 | 66.9% | 66.9% | 1167_1286del;1394_1417del;1872_1872delAins709 |
| 11 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XR_002957155.1 | 45.8% | (many diffs) | |
| 12 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | NM_009006.3 | 87.1% | 93.4% | (many diffs) |
| 13 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531755.2 | 77.5% | 82.3% | (many diffs) |
| 14 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531756.2 | 77.5% | 82.3% | (many diffs) |
| 15 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531757.2 | 77.5% | 82.3% | (many diffs) |
| 16 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_017318211.1 | 77.4% | 83.1% | (many diffs) |
| 17 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_017318212.1 | 77.4% | 83.1% | (many diffs) |
| 18 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | NM_001291787.1 | 77% | 82.5% | (many diffs) |
| 19 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531760.3 | 77% | 82.5% | (many diffs) |
| 20 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531758.3 | 76.3% | 82.2% | (many diffs) |
| 21 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XR_001782591.1 | 45.7% | (many diffs) | |
| 22 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | NR_117093.1 | 45.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2508
- ORF length:
- 2436
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgctg ctgcgggatg tgtcgctgca ggacccgcgg gaccgcttcg 121 agctgctgca gcgcgtgggg gccgggacct atggcgacgt ctacaaggcc cgcgacacgg 181 tcacgtccga actggccgcc gtgaagatag tcaagctaga cccaggggac gacatcagct 241 ccctccagca ggaaatcacc atcctgcgtg agtgccgcca ccccaatgtg gtggcctaca 301 ttggcagcta cctcaggaat gaccgcttgt ggatctgcat ggagttctgc ggagggggct 361 ccctgcagga gatttaccat gccactgggc ccctggagga gcggcagatt gcctacgtct 421 gccgagaggc actgaagggg ctccaccacc tgcattctca ggggaagatc cacagagaca 481 tcaagggagc caaccttctc ctcactctcc agggagatgt caaactggct gactttgggg 541 tgtcaggcga gctgacagcg tctgtggcca agaggaggtc tttcattggg actccctact 601 ggatggctcc cgaggtggct gctgtggagc gcaaaggtgg ctacaatgag ctatgtgacg 661 tctgggccct gggcatcact gccattgagc tgggcgagct gcagccccct ctgttccacc 721 tgcaccccat gagggccctg atgctcatgt cgaagagcag cttccagccg cccaaactga 781 gagataagac tcgctggacc cagaatttcc accactttct caaactggcc ctgaccaaga 841 atcctaagaa gaggccgaca gcagagaagc tcctgcagca cccgttcacg actcagcagc 901 tccctcgggc cctcctcaca cagctgctgg acaaagccag tgaccctcat ctggggaccc 961 cctcccctga ggactgtgag ctggagacct atgacatgtt tccagacacc attcactccc 1021 gggggcagca cggcccagcc gagaggaccc cctcggagat ccagtttcac caggtgaaat 1081 ttggcgcccc acgcaggaag gaaactgacc cactgaatga gccgtgggag gaagagtgga 1141 cactactggg aaaggaagag ttgagtggga gcctgctgca gtcggtccag gaggccctgg 1201 aggaaaggag tctgactatt cggtcagcct cagaattcca ggagctggac tccccagacg 1261 ataccatggg aaccatcaag cgggccccgt tcctagggcc actccccact gaccctccag 1321 cagaggagcc tctgtccagt cccccaggcc ccaacagctc cccactgctg cccacggcct 1381 gggccaccat gaagcagcgg gaggatcctg agaggtcatc ctgccacggg ctccccccaa 1441 ctcccaaggt gcatatgggc gcctgcttct ccaaggtctt caatggctgc cccctgcgga 1501 tccacgctgc tgtcacctgg attcaccctg ttactcggga ccagttcctg gtggtagggg 1561 ccgaggaagg catctacaca ctcaacctgc atgaactgca tgaggatacg ctggagaagc 1621 tgatttcaca tcgctgctcc tggctctact gcgtgaacaa cgtgctgctg tcactctcag 1681 ggaaatccac gcacatctgg gcccatgacc tcccaggcct gtttgagcag cggaggctac 1741 agcaacaggt tcccctctcc atccccacca accgcctcac ccagcgcatc atccccaggc 1801 gctttgctct gtccaccaag attcctgaca ccaaaggctg cttgcagtgt cgtgtggtgc 1861 ggaatcccta cacgggtgcc accttcctgc tggccgccct gcccaccagc ctgctcctgc 1921 tgcagtggta tgagccgctg cagaagtttc tgctgctgaa gaacttctcc agccctctgc 1981 ccagcccagc tgggatgctg gagccgctgg tgctggatgg gaaggagctg ccgcaggtgt 2041 gtgttggggc cgaggggcct gaggggcccg gctgccgcgt cctgttccat gtcctgcccc 2101 tggaggctgg cctgacgccc gacatcctca tcccacctga ggggatccca ggctcggccc 2161 agcaggtgat ccaggtggac agggacacaa TCCTAGTCAG CTTTGAACGC TGTGTGAGGA 2221 TTGTCAACAT GCAGGGCGAG CCCACGGCCA CACTGGCACC TGAGCTGACC TTTGATTTCC 2281 CCATCGAGAC TGTGGTGTGC CTGCAGGACA GTGTGCTGGC CTTCTGGAGC CATGGGATGC 2341 AAGGCCGAAG CCTGGATACC AATGAGGTGA CCCAGGAGAT CACAGATGAA ACAAGGATCT 2401 TCCGAGTGCT TGGGGCCCAC AGAGACATCA TCCTGGAGAG CATTCCCACT GACAACCCAG 2461 AGGCGCACAG CAACCTCTAC ATCCTCACGG GCCACCAGAG CACCTACTAG GACCCAGCTT 2521 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2581 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2641 CTTGTGGAAA GGACGAATAA GTAATATTGA TTAAGTAGCT ACGCGTTAAG TCgacaatca 2701 acctctggat tacaaaattt gtgaaagatt