Construct: ORF TRCN0000488757
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019679.1_s317c1
- DNA Barcode:
- GGTGGCTATACACACTGAAACTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK33 (65975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488757
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289061.2 | 99.5% | 99.2% | (many diffs) |
2 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352387.2 | 99.5% | 99.2% | (many diffs) |
3 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352388.2 | 99.5% | 99.2% | (many diffs) |
4 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352389.2 | 99.5% | 99.2% | (many diffs) |
5 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352390.2 | 99.5% | 99.2% | (many diffs) |
6 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352391.2 | 99.5% | 99.2% | (many diffs) |
7 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352392.2 | 99.5% | 99.2% | (many diffs) |
8 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352393.2 | 99.5% | 99.2% | (many diffs) |
9 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_030906.4 | 99.5% | 99.2% | (many diffs) |
10 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520290.2 | 99.5% | 99.2% | (many diffs) |
11 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520295.2 | 99.5% | 99.2% | (many diffs) |
12 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018144.2 | 99.5% | 99.2% | (many diffs) |
13 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018145.1 | 99.5% | 99.2% | (many diffs) |
14 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018146.1 | 99.5% | 99.2% | (many diffs) |
15 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018147.1 | 99.5% | 99.2% | (many diffs) |
16 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018148.1 | 99.5% | 99.2% | (many diffs) |
17 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018152.1 | 99.5% | 99.2% | (many diffs) |
18 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018153.2 | 99.5% | 99.2% | (many diffs) |
19 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018154.1 | 99.5% | 99.2% | (many diffs) |
20 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018155.2 | 99.5% | 99.2% | (many diffs) |
21 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018156.1 | 99.5% | 99.2% | (many diffs) |
22 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289058.2 | 91.5% | 91.2% | (many diffs) |
23 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352394.2 | 91.5% | 91.2% | (many diffs) |
24 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352395.2 | 91.5% | 91.2% | (many diffs) |
25 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520297.2 | 91.5% | 91.2% | (many diffs) |
26 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018157.1 | 91.5% | 91.2% | (many diffs) |
27 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352399.2 | 86.8% | 86.7% | (many diffs) |
28 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018159.1 | 86.8% | 86.7% | (many diffs) |
29 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018161.1 | 78.2% | 72.3% | (many diffs) |
30 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352396.2 | 70.4% | 64.6% | (many diffs) |
31 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352397.2 | 70.4% | 64.6% | (many diffs) |
32 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352398.2 | 70.4% | 64.6% | (many diffs) |
33 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289059.2 | 63.3% | 62.9% | (many diffs) |
34 | human | 65975 | STK33 | serine/threonine kinase 33 | XR_001747938.1 | 34.6% | (many diffs) | |
35 | human | 65975 | STK33 | serine/threonine kinase 33 | XR_001747939.1 | 25.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1620
- ORF length:
- 1545
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggct gatagtggct tagataaaaa atccacaaaa tgccccgact 121 gttcatctgc ttctcagaaa gatgtacttt gtgtatgttc cagcaaaaca agggttcctc 181 cagttttggt ggtggaaatg tcacagacat caagcattgg tagtgcagaa tctttaattt 241 cactggagag aaaaaaagaa aaaaatatca acagagatat aacctccagg aaagatttgc 301 cctcaagaac ctcaaatgta gagagaaaag catctcagca acaatggggt cggggcaact 361 ttacagaagg aaaagttcct cacataagga ttgagaatgg agctgctatt gaggaaatct 421 atacctttgg aagaatattg ggaaaaggga gctttggaat agtcattgaa gctacagaca 481 aggaaacaga aacgaagtgg gcaattaaaa aagtgaacaa agaaaaggct ggaagctccg 541 ctgtgaagtt acttgaacga gaggtgaaca ttctgaaaag tgtaaaacat gaacacatca 601 tacatctgga acaagtattt gaaacgccaa agaaaatgta ccttgtgatg gagctttgtg 661 aggatggaga actcaaagaa attctggata ggaaagggca tttctcagag aatgagacaa 721 ggtggatcat tcaaagtctc gcatcagcta tagcatatct tcacaataat gatattgtac 781 atagggatct gaaactggaa aatataatgg ttaaaagcag tcttattgat gataacaatg 841 aaataaactt aaacataaag gtgactgatt ttggcttagc ggtgaagaag caaagtagga 901 gtgaagccat gctgcaggcc acatgtggga ctcctatcta tatggcccct gaagttatca 961 gtgcccacga ctatagccag cagtgtgaca tttggagcat aggcgtcgta atgtacatgt 1021 tattacgtgg agaaccaccc tttttggcaa gctcagaaga gaagcttttt gagttaataa 1081 gaaaaggaga actacatttt gaaaatgcag tctggaattc cataagtgac tgtgctaaaa 1141 gtgttttgaa acaacttatg aaagtagatc ctgctcacag aatcacagct aaggaactac 1201 tagataacca gtggttaaca ggcaataaac tttcttcggt gagaccaacc aatgtattag 1261 agatgatgaa ggaatGGAAA AATAACCCAG AAAGTGTTGA GGAAAACACA ACAGAAGAGA 1321 AGAATAAGCC GTCCACTGAA GAAAAGTTGA AAAGTTACCA ACCCTGGGGA AATGTCCCTG 1381 AGACCAATTA CACTTCAGAT GAAGAGGAGG AAAAACAGTC TACTACTTAT GAAAAGCAAT 1441 TTCCTGCAAC CAGTAAGGAC AACTTTGATA TGTGCAGTTC AAGTTTCACA TCTAGCAAAC 1501 TCCTTCCAGC TGAAATCAAG GGAGAAATGG AGAAAACCCC TGTGACTCCA AGCCAAGGAA 1561 CAGCAACCAA GTACCCTGCT AAATCCGGCG CCCTGTCCAG AACCAAAAAG AAACTCGACC 1621 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1681 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1741 ATATATCTTG TGGAAAGGAC GAGGTGGCTA TACACACTGA AACTCTACGC GTTAAGTCga 1801 caatcaacct ctggattaca aaatttgtga aagatt