Construct: ORF TRCN0000488770
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020922.1_s317c1
- DNA Barcode:
- CGCGTCGATCAGTACTCTATTATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HTR2B (3357)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488770
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | NM_000867.5 | 99.8% | 99.5% | 640T>C;1430A>G |
| 2 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | XM_006712482.3 | 92.7% | 92.5% | (many diffs) |
| 3 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | NM_001320758.2 | 91.1% | 90.8% | 0_1ins126;514T>C;1304A>G |
| 4 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | XM_005246520.4 | 75.1% | 74.8% | 0_1ins357;283T>C;1073A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1515
- ORF length:
- 1443
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctctc tcttacagag tgtctgaact tcaaagcaca attcctgagc 121 acattttgca gagcaccttt gttcacgtta tctcttctaa ctggtctgga ttacagacag 181 aatcaatacc agaggaaatg aaacagattg ttgaggaaca gggaaataaa ctgcactggg 241 cagctcttct gatactcatg gtgataatac ccacaattgg tggaaatacc cttgttattc 301 tggctgtttc actggagaag aagctgcagt atgctactaa ttactttcta atgtccttgg 361 cggtggctga tttgctggtt ggattgtttg tgatgccaat tgccctcttg acaataatgt 421 ttgaggctat gtggcccctc ccacttgttc tatgtcctgc ctggttattt cttgacgttc 481 tcttttcaac cgcatccatc atgcatctct gtgccatttc agtggatcgt tacatagcca 541 tcaaaaagcc aatccaggcc aatcaatata actcacgggc tacagcattc atcaagatta 601 cagtggtgtg gttaatttca ataggcattg ccattccagt ccctattaaa gggatagaga 661 ctgatgtgga caacccaaac aatatcactt gtgtgctgac aaaggaacgt cttggcgatt 721 tcatgctctt tggctcactg gctgccttct tcacacctct tgcaattatg attgtcacct 781 actttctcac tatccatgct ttacagaaga aggcttactt agtcaaaaac aagccacctc 841 aacgcctaac atggttgact gtgtctacag ttttccaaag ggatgaaaca ccttgctcgt 901 caccggaaaa ggtggcaatg ctggatggtt ctcgaaagga caaggctctg cccaactcag 961 gtgatgaaac acttatgcga agaacatcca caattgggaa aaagtcagtg cagaccattt 1021 ccaacgaaca gagagcctca aaggtcctag ggattgtgtt tttcctcttt ttgcttatgt 1081 ggtgtccctt ctttattaca aatataactt tagttttatg tgattcctgt aaccaaacta 1141 ctctccaaat gctcctggag atatttgtgt ggataggcta tgtttcctca ggagtgaatc 1201 ctttggtcta cacccTCTTC AATAAGACAT TTCGGGATGC ATTTGGCCGA TATATCACCT 1261 GCAATTACCG GGCCACAAAG TCAGTAAAAA CTCTCAGAAA ACGCTCCAGT AAGATCTACT 1321 TCCGGAATCC AATGGCAGAG AACTCTAAGT TTTTCAAGAA ACATGGAATT CGAAATGGGA 1381 TTAACCCTGC CATGTACCAG AGTCCAATGA GGCTCCGAAG TTCAACCATT CAGTCTTCAT 1441 CAATCATTCT ACTAGATACG CTTCTCCTCA CTGAAAATGA AGGTGACAAA ACTGAAGAGC 1501 GAGTTAGTTA TGTATAGGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1561 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1621 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGCGTCG ATCAGTACTC 1681 TATTATAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt