Construct: ORF TRCN0000488771
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020877.1_s317c1
- DNA Barcode:
- AACGCGCTTTCATGGCAAGTGTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HTR1A (3350)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488771
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3350 | HTR1A | 5-hydroxytryptamine recepto... | NM_000524.3 | 100% | 100% | |
| 2 | mouse | 15550 | Htr1a | 5-hydroxytryptamine (seroto... | NM_008308.4 | 85.4% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1338
- ORF length:
- 1266
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatgtg ctcagccctg gtcagggcaa caacaccaca tcaccaccgg 121 ctccctttga gaccggcggc aacactactg gtatctccga cgtgaccgtc agctaccaag 181 tgatcacctc tctgctgctg ggcacgctca tcttctgcgc ggtgctgggc aatgcgtgcg 241 tggtggctgc catcgccttg gagcgctccc tgcagaacgt ggccaattat cttattggct 301 ctttggcggt caccgacctc atggtgtcgg tgttggtgct gcccatggcc gcgctgtatc 361 aggtgctcaa caagtggaca ctgggccagg taacctgcga cctgttcatc gccctcgacg 421 tgctgtgctg cacctcatcc atcttgcacc tgtgcgccat cgcgctggac aggtactggg 481 ccatcacgga ccccatcgac tacgtgaaca agaggacgcc ccggcgcgcc gctgcgctca 541 tctcgctcac ttggcttatt ggcttcctca tctctatccc gcccatgctg ggctggcgca 601 ccccggaaga ccgctcggac cccgacgcat gcaccattag caaggatcat ggctacacta 661 tctattccac ctttggagct ttctacatcc cgctgctgct catgctggtt ctctatgggc 721 gcatattccg agctgcgcgc ttccgcatcc gcaagacggt caaaaaggtg gagaagaccg 781 gagcggacac ccgccatgga gcatctcccg ccccgcagcc caagaagagt gtgaatggag 841 agtcggggag caggaactgg aggctgggcg tggagagcaa ggctgggggt gctctgtgcg 901 ccaatggcgc ggtgaggcaa ggtgacgatg gcgccgccct ggaggtgatc gaggtgcacc 961 gagtgggcaa ctccaaagag cacttgcctc tgcccagcga ggctggtcct accccttgtg 1021 cccccgccTC TTTCGAGAGG AAAAATGAGC GCAACGCCGA GGCGAAGCGC AAGATGGCCC 1081 TGGCCCGAGA GAGGAAGACA GTGAAGACGC TGGGCATCAT CATGGGCACC TTCATCCTCT 1141 GCTGGCTGCC CTTCTTCATC GTGGCTCTTG TTCTGCCCTT CTGCGAGAGC AGCTGCCACA 1201 TGCCCACCCT GTTGGGCGCC ATAATCAATT GGCTGGGCTA CTCCAACTCT CTGCTTAACC 1261 CCGTCATTTA CGCATACTTC AACAAGGACT TTCAAAACGC GTTTAAGAAG ATCATTAAGT 1321 GTAAGTTCTG CCGCCAGTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACG CGCTTTCATG 1501 GCAAGTGTTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt