Construct: ORF TRCN0000488777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020346.1_s317c1
- DNA Barcode:
- TGAGCGTAAACATGGCAATGCACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MKNK2 (2872)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488777
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2872 | MKNK2 | MAPK interacting serine/thr... | NM_017572.4 | 100% | 100% | |
| 2 | human | 2872 | MKNK2 | MAPK interacting serine/thr... | XM_024451471.1 | 90.1% | 90.1% | 139_273del |
| 3 | human | 2872 | MKNK2 | MAPK interacting serine/thr... | NM_199054.2 | 87.1% | 81.4% | (many diffs) |
| 4 | mouse | 17347 | Mknk2 | MAP kinase-interacting seri... | NM_021462.4 | 78.1% | 78.2% | (many diffs) |
| 5 | mouse | 17347 | Mknk2 | MAP kinase-interacting seri... | XM_006513316.1 | 78.1% | 78.2% | (many diffs) |
| 6 | mouse | 17347 | Mknk2 | MAP kinase-interacting seri... | XM_017313824.1 | 69.4% | 70.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1314
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtgcag aagaaaccag ccgaacttca gggtttccac cgttcgttca 121 aggggcagaa ccccttcgag ctggccttct ccctagacca gcccgaccac ggagactctg 181 actttggcct gcagtgctca gcccgccctg acatgcccgc cagccagccc attgacatcc 241 cggacgccaa gaagaggggc aagaagaaga agcgcggccg ggccaccgac agcttctcgg 301 gcaggtttga agacgtctac cagctgcagg aagatgtgct gggggagggc gctcatgccc 361 gagtgcagac ctgcatcaac ctgatcacca gccaggagta cgccgtcaag atcattgaga 421 agcagccagg ccacattcgg agcagggttt tcagggaggt ggagatgctg taccagtgcc 481 agggacacag gaacgtccta gagctgattg agttcttcga ggaggaggac cgcttctacc 541 tggtgtttga gaagatgcgg ggaggctcca tcctgagcca catccacaag cgccggcact 601 tcaacgagct ggaggccagc gtggtggtgc aggacgtggc cagcgccttg gactttctgc 661 ataacaaagg catcgcccac agggacctaa agccggaaaa catcctctgt gagcacccca 721 accaggtctc ccccgtgaag atctgtgact tcgacctggg cagcggcatc aaactcaacg 781 gggactgctc ccctatctcc accccggagc tgctcactcc gtgcggctcg gcggagtaca 841 tggccccgga ggtagtggag gccttcagcg aggaggctag catctacgac aagcgctgcg 901 acctgtggag cctgggcgtc atcttgtata tcctactcag cggctacccg cccttcgtgg 961 gccgctgtgg cagcgactgc ggctgggacc gcggcgaggc ctgccctgcc tgccagaaca 1021 tgctgtttga gagcaTCCAG GAGGGCAAGT ACGAGTTCCC CGACAAGGAC TGGGCCCACA 1081 TCTCCTGCGC TGCCAAAGAC CTCATCTCCA AGCTGCTGGT CCGTGACGCC AAGCAGAGGC 1141 TGAGTGCCGC CCAAGTCCTG CAGCACCCCT GGGTTCAGGG GTGCGCCCCG GAGAACACCT 1201 TGCCCACTCC CATGGTCCTG CAGAGGTGGG ACAGTCACTT CCTCCTCCCT CCCCACCCCT 1261 GTCGCATCCA CGTGCGACCT GGAGGACTGG TCAGAACCGT TACTGTGAAT GAGTGAGACC 1321 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GATGAGCGTA AACATGGCAA TGCACAACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt