Construct: ORF TRCN0000488779
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019290.1_s317c1
- DNA Barcode:
- TTTCCCCCTTCTGGGGAATTGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TACR1 (6869)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488779
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6869 | TACR1 | tachykinin receptor 1 | NM_001058.4 | 100% | 100% | |
| 2 | human | 6869 | TACR1 | tachykinin receptor 1 | NM_015727.3 | 76.4% | 76.4% | 933_934ins288 |
| 3 | mouse | 21336 | Tacr1 | tachykinin receptor 1 | NM_009313.5 | 89.7% | 94.5% | (many diffs) |
| 4 | mouse | 21336 | Tacr1 | tachykinin receptor 1 | XM_006505863.3 | 89.7% | 94.5% | (many diffs) |
| 5 | mouse | 21336 | Tacr1 | tachykinin receptor 1 | XM_006505864.3 | 89.7% | 94.5% | (many diffs) |
| 6 | mouse | 21336 | Tacr1 | tachykinin receptor 1 | XM_006505865.3 | 89.7% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1296
- ORF length:
- 1221
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggat aacgtcctcc cggtggactc agacctctcc ccaaacatct 121 ccactaacac ctcggaaccc aatcagttcg tgcaaccagc ctggcaaatt gtcctttggg 181 cagctgccta cacggtcatt gtggtgacct ctgtggtggg caacgtggta gtgatgtgga 241 tcatcttagc ccacaaaaga atgaggacag tgacgaacta ttttctggtg aacctggcct 301 tcgcggaggc ctccatggct gcattcaata cagtggtgaa cttcacctat gctgtccaca 361 acgaatggta ctacggcctg ttctactgca agttccacaa cttctttccc atcgccgctg 421 tcttcgccag tatctactcc atgacggctg tggcctttga taggtacatg gccatcatac 481 atcccctcca gccccggctg tcagccacag ccaccaaagt ggtcatctgt gtcatctggg 541 tcctggctct cctgctggcc ttcccccagg gctactactc aaccacagag accatgccca 601 gcagagtcgt gtgcatgatc gaatggccag agcatccgaa caagatttat gagaaagtgt 661 accacatctg tgtgactgtg ctgatctact tcctccccct gctggtgatt ggctatgcat 721 acaccgtagt gggaatcaca ctatgggcca gtgagatccc cggggactcc tctgaccgct 781 accacgagca agtctctgcc aagcgcaagg tggtcaaaat gatgattgtc gtggtgtgca 841 ccttcgccat ctgctggctg cccttccaca tcttcttcct cctgccctac atcaacccag 901 atctctacct gaagaagttt atccagcagg tctaccTGGC CATCATGTGG CTGGCCATGA 961 GCTCCACCAT GTACAACCCC ATCATCTACT GCTGCCTCAA TGACAGGTTC CGTCTGGGCT 1021 TCAAGCATGC CTTCCGGTGC TGCCCCTTCA TCAGCGCCGG CGACTATGAG GGGCTGGAAA 1081 TGAAATCCAC CCGGTATCTC CAGACCCAGG GCAGTGTGTA CAAAGTCAGC CGCCTGGAGA 1141 CCACCATCTC CACAGTGGTG GGGGCCCACG AGGAGGAGCC AGAGGACGGC CCCAAGGCCA 1201 CACCCTCGTC CCTGGACCTG ACCTCCAACT GCTCTTCACG AAGTGACTCC AAGACCATGA 1261 CAGAGAGCTT CAGCTTCTCC TCCAATGTGC TCTCCGACCC AGCTTTCTTG TACAAAGTGG 1321 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1381 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1441 ATTTCCCCCT TCTGGGGAAT TGCCTACGCG TTAAGTCgac aatcaacctc tggattacaa 1501 aatttgtgaa agatt