Construct: ORF TRCN0000488795
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021463.1_s317c1
- DNA Barcode:
- ACAACACCTGGCAACCAGGGGCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GNG7 (2788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488795
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2788 | GNG7 | G protein subunit gamma 7 | NM_052847.3 | 100% | 100% | |
| 2 | human | 2788 | GNG7 | G protein subunit gamma 7 | XM_017026606.1 | 100% | 100% | |
| 3 | mouse | 14708 | Gng7 | guanine nucleotide binding ... | NM_001038655.1 | 82.1% | 92.7% | (many diffs) |
| 4 | mouse | 14708 | Gng7 | guanine nucleotide binding ... | NM_010319.3 | 82.1% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 138
- ORF end:
- 342
- ORF length:
- 204
- Sequence:
-
1 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGCTAGCAT CGATTGATCA ACAAGTTTGT 121 ACAAAAAAGC AGGCTTCATG TCAGCCACTA ACAACATAGC CCAGGCCCGG AAGCTGGTGG 181 AACAGCTACG CATAGAAGCC GGGATTGAGC GCATCAAGGT CTCCAAAGCG GCGTCTGACC 241 TCATGAGCTA CTGTGAGCAA CATGCCCGGA ACGACCCCCT GCTGGTCGGA GTCCCTGCCT 301 CGGAGAACCC CTTTAAGGAC AAGAAACCTT GTATTATTTT ATAAGACCCA GCTTTCTTGT 361 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 421 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 481 GAAAGGACGA ACAACACCTG GCAACCAGGG GCACACGCGT TAAGTCgaca atcaacctct 541 ggattacaaa atttgtgaaa gatt