Construct: ORF TRCN0000488797
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021322.1_s317c1
- DNA Barcode:
- AATTAACGATGCTTCAATCTAAGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FGF7 (2252)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488797
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2252 | FGF7 | fibroblast growth factor 7 | NM_002009.4 | 49.6% | 48.4% | (many diffs) |
| 2 | mouse | 14178 | Fgf7 | fibroblast growth factor 7 | NM_008008.4 | 44.5% | 44.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 363
- ORF length:
- 291
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttCAC CATGCACAAA TGGATACTGA CATGGATCCT GCCAACTTTG CTCTACAGAT 121 CATGCTTTCA CATTATCTGT CTAGTGGGTA CTATATCTTT AGCTTGCAAT GACATGACTC 181 CAGAGCAAAT GGCTACAAAT GTGAACTGTT CCAGCCCTGA GCGACACACA AGAAGTTATG 241 ATTACATGGA AGGAGAGGAT ATAAGAGTGA GAAGACTCTT CTGTCGAACA CAGTGGTACC 301 TGAGGATCGA TAAAAGAGGC AAAGTAAAAG GGACCCAAGA GATGAAGAAT AATTACAGTA 361 AGTAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 421 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 481 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAATTAACGA TGCTTCAATC TAAGGACGCG 541 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt