Transcript: Human NM_002009.4

Homo sapiens fibroblast growth factor 7 (FGF7), mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
FGF7 (2252)
Length:
5321
CDS:
466..1050

Additional Resources:

NCBI RefSeq record:
NM_002009.4
NBCI Gene record:
FGF7 (2252)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002009.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000372263 CTAGTGGGTACTATATCTTTA pLKO_005 535 CDS 100% 0.000 0.000 N FGF7 n/a
2 TRCN0000058457 CAGATCATGCTTTCACATTAT pLKO.1 510 CDS 100% 13.200 9.240 N FGF7 n/a
3 TRCN0000058453 CCAGAGCAAATGGCTACAAAT pLKO.1 574 CDS 100% 13.200 9.240 N FGF7 n/a
4 TRCN0000058456 GACCCAAGAGATGAAGAATAA pLKO.1 726 CDS 100% 13.200 9.240 N FGF7 n/a
5 TRCN0000058455 GACACACAAGAAGTTATGATT pLKO.1 617 CDS 100% 5.625 3.938 N FGF7 n/a
6 TRCN0000067674 GTTGGAATTGTGGCAATCAAA pLKO.1 778 CDS 100% 5.625 3.375 N Fgf7 n/a
7 TRCN0000372262 TCAGGACAGTGGCAGTTGGAA pLKO_005 764 CDS 100% 3.000 1.800 N FGF7 n/a
8 TRCN0000159283 GCAAAGAAAGAATGCAATGAA pLKO.1 850 CDS 100% 5.625 2.813 Y FGF7P6 n/a
9 TRCN0000164145 CCACTTTCTTCCTATGGCAAT pLKO.1 1023 CDS 100% 4.050 2.025 Y FGF7P6 n/a
10 TRCN0000058454 CCCACTTTCTTCCTATGGCAA pLKO.1 1022 CDS 100% 2.640 1.320 Y FGF7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002009.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10821 pDONR223 100% 49.6% 48.4% None (many diffs) n/a
2 ccsbBroad304_10821 pLX_304 0% 49.6% 48.4% V5 (many diffs) n/a
3 TRCN0000467676 TCACACTCCGTCTTATAAACCGCG pLX_317 100% 49.6% 48.4% V5 (many diffs) n/a
4 TRCN0000488797 AATTAACGATGCTTCAATCTAAGG pLX_317 96.5% 49.6% 48.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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