Construct: ORF TRCN0000488805
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020323.1_s317c1
- DNA Barcode:
- TTTTCTCAAGTCATATTGCATGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TEC (7006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488805
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7006 | TEC | tec protein tyrosine kinase | NM_003215.3 | 99.8% | 100% | 453C>T;963C>A |
| 2 | human | 7006 | TEC | tec protein tyrosine kinase | XM_024454193.1 | 99.8% | 100% | 453C>T;963C>A |
| 3 | human | 7006 | TEC | tec protein tyrosine kinase | XM_011513737.2 | 94% | 92.8% | (many diffs) |
| 4 | human | 7006 | TEC | tec protein tyrosine kinase | XM_017008569.1 | 81.5% | 81.6% | 0_1ins348;105C>T;615C>A |
| 5 | human | 7006 | TEC | tec protein tyrosine kinase | XM_011513741.1 | 73.7% | 73% | (many diffs) |
| 6 | human | 7006 | TEC | tec protein tyrosine kinase | XR_001741318.1 | 51% | (many diffs) | |
| 7 | human | 7006 | TEC | tec protein tyrosine kinase | XR_925160.2 | 48.4% | (many diffs) | |
| 8 | human | 7006 | TEC | tec protein tyrosine kinase | XR_001741319.1 | 45.4% | (many diffs) | |
| 9 | mouse | 21682 | Tec | tec protein tyrosine kinase | NM_001113460.2 | 88.9% | 95% | (many diffs) |
| 10 | mouse | 21682 | Tec | tec protein tyrosine kinase | NM_001113464.2 | 88.9% | 95% | (many diffs) |
| 11 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_006503845.3 | 88.9% | 95% | (many diffs) |
| 12 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_006503846.3 | 88.9% | 95% | (many diffs) |
| 13 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_011240718.2 | 88.9% | 95% | (many diffs) |
| 14 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_011240719.2 | 88.9% | 95% | (many diffs) |
| 15 | mouse | 21682 | Tec | tec protein tyrosine kinase | NM_001113461.2 | 85.7% | 91.7% | (many diffs) |
| 16 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_006503847.2 | 84.1% | 88.2% | (many diffs) |
| 17 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_006503848.3 | 77.2% | 81.2% | (many diffs) |
| 18 | mouse | 21682 | Tec | tec protein tyrosine kinase | NM_013689.5 | 74.1% | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1965
- ORF length:
- 1893
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaatttt aacactattt tggaggagat tcttattaaa aggtcacagc 121 agaaaaagaa gacatcgccc ttaaactaca aagagagact ttttgtactt acaaagtcca 181 tgctaaccta ctatgagggt cgagcagaga agaaatacag aaaggggttt attgatgttt 241 caaaaatcaa gtgtgtggaa atagtgaaga atgatgatgg tgtcattccc tgtcaaaata 301 agtatccatt tcaggttgtt catgatgcta acacacttta catttttgca cctagtccac 361 aaagcaggga cctgtgggtg aagaagttaa aagaagaaat aaagaacaac aataatatta 421 tgattaaata tcatcctaaa ttctggacag atggaagtta tcagtgttgt agacaaactg 481 aaaaattagc acccggatgt gaaaaataca atctttttga gagtagtata agaaaagcac 541 tacctccagc accagaaaca aagaagcgaa ggcctccccc accaattcca ctagaagaag 601 aagataatag tgaagaaatc gttgtagcca tgtatgattt ccaagcagca gaaggacatg 661 atctcagatt agagagaggc caagagtatc tcattttaga aaagaatgat gttcattggt 721 ggagagcaag agataaatat gggaatgaag gatatatccc aagtaattac gtaacgggaa 781 agaaatcaaa caacttagat caatatgaat ggtattgcag aaatatgaat agaagcaagg 841 cagagcaact cctccgcagt gaagataaag aaggtggttt tatggtaagg gattccagtc 901 aaccaggctt gtacacagtc tccctttata ccaagtttgg aggagaaggt tcatcgggtt 961 ttaggcatta tcatataaag gaaacaacaa catctccaaa gaagtattac ctagctgaaa 1021 aacatgcttt tggatccatt cctgagatta ttgaatatca taagcacaat gcagcaggac 1081 ttgtcaccag gcttcggtac ccagttagtg tgaaagggaa gaatgcaccc accactgcag 1141 gattcagcta tgagaaatgg gagattaacc cttcagaact gacctttatg agggaattgg 1201 gaagtggact gtttggagtg gtgaggcttg gcaaatggcg agcccagtac aaagtcgcaa 1261 tcaaagctat tcgggaaggt gcaatgtgcg aggaggactt tatagaagaa gctaaagtga 1321 tgatgaaact gacacacccg aagttagtgc agctttatgg tgtgtgcacc cagcagaaac 1381 caatatacat tgttactgag ttcatggaaa ggggctgcct tctgaatttc ctccgacaga 1441 gacaaggtca tttcagtaga gacgtactgc tgagcatgtg tcaggatgtg tgtgaaggga 1501 tggagtatct ggagagaaac agcttcatcc acagagatct ggctgccaga aattgtctag 1561 taagtgaggc gggagttgta aaagtatctg attttggaat ggccaggtat gttctggatg 1621 atcagtacac aagttcttct ggtgctaagt ttCCTGTGAA GTGGTGTCCA CCTGAAGTGT 1681 TTAATTACAG CCGCTTCAGC AGCAAATCAG ATGTCTGGTC ATTTGGTGTT TTAATGTGGG 1741 AAGTATTCAC GGAAGGCAGA ATGCCTTTTG AAAAATACAC CAATTATGAA GTGGTAACCA 1801 TGGTTACTCG AGGCCACCGA CTCTACCAGC CGAAGTTGGC GTCCAACTAT GTGTATGAGG 1861 TGATGCTGAG ATGTTGGCAG GAGAAACCAG AGGGAAGGCC TTCTTTCGAA GATCTGCTGC 1921 GCACAATAGA TGAACTAGTT GAATGTGAAG AAACTTTTGG AAGATGAGAC CCAGCTTTCT 1981 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2041 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2101 GTGGAAAGGA CGATTTTCTC AAGTCATATT GCATGAAACG CGTTAAGTCg acaatcaacc 2161 tctggattac aaaatttgtg aaagatt