Construct: ORF TRCN0000488806
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020280.1_s317c1
- DNA Barcode:
- TGAAGTCGAGCTTAGTCCGTATAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- VRK2 (7444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488806
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130480.2 | 99.8% | 99.8% | 1524_1525insTTG |
| 2 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130481.2 | 99.8% | 99.8% | 1524_1525insTTG |
| 3 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288837.1 | 99.8% | 99.8% | 1524_1525insTTG |
| 4 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_006296.6 | 99.8% | 99.8% | 1524_1525insTTG |
| 5 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_005264540.4 | 99.8% | 99.8% | 1524_1525insTTG |
| 6 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_011533092.3 | 99.8% | 99.8% | 1524_1525insTTG |
| 7 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130482.2 | 95.2% | 95.2% | 0_1ins69;1455_1456insTTG |
| 8 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712090.4 | 77.6% | 77.4% | 1184_1186delTAGinsA;1191_1191delTins339 |
| 9 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004858.2 | 77.6% | 77.4% | 1184_1186delTAGinsA;1191_1191delTins339 |
| 10 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130483.2 | 77.5% | 77.4% | 1183_1185delTTTinsGAA;1188_1188delGins340 |
| 11 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288838.1 | 77.5% | 77.4% | 1183_1185delTTTinsGAA;1188_1188delGins340 |
| 12 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712091.4 | 77.5% | 77.4% | 1183_1185delTTTinsGAA;1188_1188delGins340 |
| 13 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288836.1 | 76.6% | 76.6% | 0_1ins354;1170_1171insTTG |
| 14 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288839.1 | 76.6% | 76.6% | 0_1ins354;1170_1171insTTG |
| 15 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712092.4 | 76.6% | 76.6% | 0_1ins354;1170_1171insTTG |
| 16 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712093.4 | 76.6% | 76.6% | 0_1ins354;1170_1171insTTG |
| 17 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004859.2 | 76.6% | 76.6% | 0_1ins354;1170_1171insTTG |
| 18 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004860.2 | 76.6% | 76.6% | 0_1ins354;1170_1171insTTG |
| 19 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004861.2 | 76.6% | 76.6% | 0_1ins354;1170_1171insTTG |
| 20 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004862.2 | 76.6% | 76.6% | 0_1ins354;1170_1171insTTG |
| 21 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004863.2 | 76.6% | 76.6% | 0_1ins354;1170_1171insTTG |
| 22 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004865.1 | 54.3% | 54.2% | 0_1ins354;829_831delTTTinsGAA;834_834delGins340 |
| 23 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004866.1 | 54.3% | 54.2% | 0_1ins354;829_831delTTTinsGAA;834_834delGins340 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1599
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccacca aaaagaaatg aaaaatacaa acttcctatt ccatttccag 121 aaggcaaggt tctggatgat atggaaggca atcagtgggt actgggcaag aagattggct 181 ctggaggatt tggattgata tatttagctt tccccacaaa taaaccagag aaagatgcaa 241 gacatgtagt aaaagtggaa tatcaagaaa atggcccgtt attttcagaa cttaaatttt 301 atcagagagt tgcaaaaaaa gactgtatca aaaagtggat agaacgcaaa caacttgatt 361 atttaggaat tcctctgttt tatggatctg gtctgactga attcaaggga agaagttaca 421 gatttatggt aatggaaaga ctaggaatag atttacagaa gatctcaggc cagaatggta 481 cctttaaaaa gtcaactgtc ctgcaattag gtatccgaat gttggatgta ctggaatata 541 tacatgaaaa tgaatatgtt catggtgata taaaagcagc aaatctactt ttgggttaca 601 aaaatccaga ccaggtttat cttgcagatt atggactttc ctacagatat tgtcccaatg 661 ggaaccacaa acagtatcag gaaaatccta gaaaaggcca taatgggaca atagagttta 721 ccagcttgga tgcccacaag ggagtagcct tgtccagacg aagtgacgtt gagatcctcg 781 gctactgcat gctgcggtgg ttgtgtggga aacttccctg ggaacagaac ctgaaggacc 841 ctgtggctgt gcagactgct aaaacaaatc tgttggacga gctcccccag tcagtgctta 901 aatgggctcc ttctggaagc agttgctgtg aaatagccca atttttggta tgtgctcata 961 gtttagcata tgatgaaaag ccaaactatc aagccctcaa gaaaattttg aaccctcatg 1021 gaataccttt aggaccactg gacttttcca caaaaggaca gagtataaat gtccatactc 1081 caaacagtca aaaagttgat tcacaaaagg ctgcaacaaa gcaagtcaac aaggcacaca 1141 ataggttaat cgaaaaaaaa gtccacagtg agagaagcgc tgagtcctgt gcaacatgga 1201 aagtgcagaa agaggagaaa ctgattggat tgatgaacaa tgaagcagct caggaaagca 1261 caaggagaag acagaaatat caagagtctc aagaaccttt gaatGAAGTA AACAGTTTCC 1321 CACAAAAAAT CAGCTATACA CAATTCCCAA ACTCATTTTA TGAGCCTCAT CAAGATTTTA 1381 CCAGTCCAGA TATATTCAAG AAGTCAAGAT CTCCATCTTG GTATAAATAC ACTTCCACAG 1441 TCAGCACGGG GATCACAGAC TTAGAAAGTT CAACTGGACT TTGGCCTACA ATTTCCCAGT 1501 TTACTCTTAG TGAAGAGACA AACGCAGATG TTTATTATTA TCGCATCATC ATACCTGTCC 1561 TTTTGATGTT AGTATTTCTT GCTTTATTTT TTCTCTTGTG AGACCCAGCT TTCTTGTACA 1621 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1681 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1741 AGGACGATGA AGTCGAGCTT AGTCCGTATA AACGCGTTAA GTCgacaatc aacctctgga 1801 ttacaaaatt tgtgaaagat t