Construct: ORF TRCN0000488819

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019387.1_s317c1
DNA Barcode:
AGCGTGAACCTTCTCTCTCCGGGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GPR4 (2828)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488819
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2828 GPR4 G protein-coupled receptor 4 NM_005282.3 99.9% 99.7% 1086_1087insG
2 human 2828 GPR4 G protein-coupled receptor 4 XM_017026607.2 99.9% 99.7% 1086_1087insG
3 human 2828 GPR4 G protein-coupled receptor 4 XM_017026608.1 99.9% 99.7% 1086_1087insG
4 mouse 319197 Gpr4 G protein-coupled receptor 4 NM_175668.4 86.6% 91.2% (many diffs)
5 mouse 319197 Gpr4 G protein-coupled receptor 4 XM_006540048.3 86.6% 91.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
1164
ORF length:
1089
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatgggc aaccacacgt gggagggctg ccacgtggac tcgcgcgtgg
121accacctctt tccgccatcc ctctacatct ttgtcatcgg cgtggggctg cccaccaact
181gcctggctct gtgggcggcc taccgccagg tgcaacagcg caacgagctg ggcgtctacc
241tgatgaacct cagcatcgcc gacctgctgt acatctgcac gctgccgctg tgggtggact
301acttcctgca ccacgacaac tggatccacg gccccgggtc ctgcaagctc tttgggttca
361tcttctacac caatatctac atcagcatcg ccttcctgtg ctgcatctcg gtggaccgct
421acctggctgt ggcccaccca ctccgcttcg cccgcctgcg ccgcgtcaag accgccgtgg
481ccgtgagctc cgtggtctgg gccacggagc tgggcgccaa ctcggcgccc ctgttccatg
541acgagctctt ccgagaccgc tacaaccaca ccttctgctt tgagaagttc cccatggaag
601gctgggtggc ctggatgaac ctctatcggg tgttcgtggg cttcctcttc ccgtgggcgc
661tcatgctgct gtcgtaccgg ggcatcctgc gggccgtgcg gggcagcgtg tccaccgagc
721gccaggagaa ggccaagatc aagcggctgg ccctcagcct catcgccatc gtgctggtct
781gctttgcgcc ctatcacgtg ctcttgctgt cccgcagcgc catctacctg ggccgcccct
841GGGACTGCGG CTTCGAGGAG CGCGTCTTTT CTGCATACCA CAGCTCACTG GCTTTCACCA
901GCCTCAACTG TGTGGCGGAC CCCATCCTCT ACTGCCTGGT CAACGAGGGC GCCCGCAGCG
961ATGTGGCCAA GGCCCTGCAC AACCTGCTCC GCTTTCTGGC CAGCGACAAG CCCCAGGAGA
1021TGGCCAATGC CTCGCTCACC CTGGAGACCC CACTCACCTC CAAGAGGAAC AGCACAGCCA
1081AAGCCATGAC TGGCAGCTGG GCGGCCACTC CGCCCTCCCA GGGGGACCAG GTGCAGCTGA
1141AGATGCTGCC GCCAGCACAA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1201CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1261AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGCG TGAACCTTCT
1321CTCTCCGGGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)