Construct: ORF TRCN0000488828
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021425.1_s317c1
- DNA Barcode:
- AACTGCCTATCTGGCAAAGACCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SSH2 (85464)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488828
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_017025253.2 | 94.8% | 94.8% | 64_144del |
| 2 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_017025254.2 | 84.9% | 84.6% | (many diffs) |
| 3 | human | 85464 | SSH2 | slingshot protein phosphata... | NM_001282130.2 | 37.5% | 36.2% | (many diffs) |
| 4 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_017025255.2 | 35.6% | 34.4% | (many diffs) |
| 5 | human | 85464 | SSH2 | slingshot protein phosphata... | NM_033389.3 | 34% | 32% | (many diffs) |
| 6 | human | 85464 | SSH2 | slingshot protein phosphata... | NM_001282131.2 | 33.9% | 29.7% | (many diffs) |
| 7 | human | 85464 | SSH2 | slingshot protein phosphata... | NM_001282129.2 | 33.4% | 31.4% | (many diffs) |
| 8 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_005258058.3 | 33.4% | 31.4% | (many diffs) |
| 9 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_017025251.2 | 33.3% | 31.4% | (many diffs) |
| 10 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525402.2 | 32.9% | 30.6% | (many diffs) |
| 11 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_005258059.2 | 32.7% | 29.7% | (many diffs) |
| 12 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_006722149.4 | 32.1% | 29.8% | (many diffs) |
| 13 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525404.2 | 32.1% | 29.6% | (many diffs) |
| 14 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525405.2 | 31.8% | 29.6% | (many diffs) |
| 15 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525406.2 | 28.7% | 25.8% | (many diffs) |
| 16 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525407.1 | 28.7% | 25.8% | (many diffs) |
| 17 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_017025252.1 | 27.4% | 23.1% | (many diffs) |
| 18 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | NM_177710.4 | 31.8% | 31.6% | (many diffs) |
| 19 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | XM_006533227.3 | 31.2% | 31% | (many diffs) |
| 20 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | XM_006533228.3 | 31.2% | 30.9% | (many diffs) |
| 21 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | XM_006533229.3 | 30.6% | 29.6% | (many diffs) |
| 22 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | NM_001291190.1 | 30.5% | 29.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 87
- ORF end:
- 1593
- ORF length:
- 1506
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcgg 61 ccgccccctt caccggatcc cggaccatgg ctttggtcac ggtccagcgg tcacctaccc 121 ccagcaccac ctccagcccc tgcgcctcgg aggcagacag tggggaggaa gaatgccggt 181 cacagcccag gagcatcagc gagagctttc taactgtcaa aggtgctgcc ctttttctac 241 cacggggaaa tggctcatcc acaccaagaa tcagccacag acggaacaag catgcaggcg 301 atctccaaca gcatctccaa gcaatgttca ttttactccg cccagaagac aacatcaggc 361 tggctgtaag actggaaagt acttaccaga atcgaacacg ctatatggta gtggtttcaa 421 ctaatggtag acaagacact gaagaaagca tcgtcctagg aatggatttc tcctctaatg 481 acagtagcac ttgtaccatg ggcttagttt tgcctctctg gagcgacacg ctaattcatt 541 tggatggtga tggtgggttc agtgtatcga cggataacag agttcacata ttcaaacctg 601 tatctgtgca ggcaatgtgg tctgcactac agagcttaca caaggcttgt gaagtcgcca 661 gagcgcataa ctactaccca ggcagcctat ttctcacttg ggtgagttat tatgagagcc 721 atatcaactc agatcaatcc tcagtcaatg aatggaatgc aatgcaagat gtacagtccc 781 accggcccga ctctccagct ctcttcaccg acatacctac tgaacgtgaa cgaacagaaa 841 ggctaattaa aaccaaatta agggagatca tgatgcagaa ggatttggag aatattacat 901 ccaaagagat aagaacagag ttggaaatgc aaatggtgtg caacttgcgg gaattcaagg 961 aatttataga caatgaaatg atagtgatcc ttggtcaaat ggatagccct acacagatat 1021 ttgagcatgt gttcctgggc tcagaatgga atgcctccaa cttagaggac ttacagaacc 1081 gaggggtacg gtatatcttg aatgtcactc gagagataga taacttcttc ccaggagtct 1141 ttgagtatca taacattcgg gtatatgatg aagaggcaac ggatctcctg gcgtactgga 1201 atgacactta caaattcatc tctaaagcaa agaaacatgg atctaaatgc cttgtgcact 1261 gcaaaatggg ggtgagtcgc tcagcctcca ccgtgattGC CTATGCAATG AAGGAATATG 1321 GCTGGAATCT GGACCGAGCC TATGACTATG TGAAAGAAAG ACGAACGGTA ACCAAGCCCA 1381 ACCCAAGCTT CATGAGACAA CTGGAAGAGT ATCAGGGGAT CTTGCTGGCA AGCTTCCTAG 1441 GCTTGATTCA TGGAGGGAGG GACAAGCCCT GGGGAGAGAA AAGCACAGAA TTTGAGTCAG 1501 TAGATCTGGT TTCCATTCCT GGTTCACCCT CTTGCTGCAA CCCTGAGAAG TTACTTCACA 1561 TTTCTCATCC TTACCTGACC CCATCTATAA AATGAAAGGG TGGGCGCGCC GACCCAGCTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGAAACTGCC TATCTGGCAA AGACCTCACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt