Construct: ORF TRCN0000488843

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020189.1_s317c1
DNA Barcode:
GAACCTGCCAACTGGCAGTAGAAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
AK1 (203)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488843
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 203 AK1 adenylate kinase 1 NM_000476.2 98.9% 98.4% 27G>T;580_581delAAinsTT;582_583insAAT
2 human 203 AK1 adenylate kinase 1 NM_001318121.1 98.9% 98.4% 27G>T;580_581delAAinsTT;582_583insAAT
3 human 203 AK1 adenylate kinase 1 XM_017014428.1 98.8% 98.4% (many diffs)
4 human 203 AK1 adenylate kinase 1 XM_024447439.1 91.9% 92.3% (many diffs)
5 human 203 AK1 adenylate kinase 1 NM_001318122.2 91.1% 90% (many diffs)
6 human 203 AK1 adenylate kinase 1 XM_024447440.1 68.2% 68.2% (many diffs)
7 mouse 11636 Ak1 adenylate kinase 1 NM_001198790.1 85.3% 87.1% (many diffs)
8 mouse 11636 Ak1 adenylate kinase 1 NM_001198791.1 85.3% 87.1% (many diffs)
9 mouse 11636 Ak1 adenylate kinase 1 NM_001198792.1 85.3% 87.1% (many diffs)
10 mouse 11636 Ak1 adenylate kinase 1 XM_006497624.3 85.3% 87.1% (many diffs)
11 mouse 11636 Ak1 adenylate kinase 1 NM_021515.3 78.4% 79.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
657
ORF length:
585
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggaagag aagctgaaga aaaccaatat catctttgtg gtgggtgggc
121ctggctcagg gaagggcacc cagtgtgaga agatcgtgca gaagtatggc tacacccacc
181tctccaccgg ggacctcctg cggtccgagg tcagctcagg ctcggccagg ggcaagaagc
241tgtcggaaat catggagaag gggcagctgg ttccactgga gacagtgttg gacatgctcc
301gggatgccat ggtggccaaa gtcaatactt ccaaaggctt ccTGATTGAT GGCTACCCGC
361GGGAGGTGCA GCAAGGAGAA GAGTTTGAGC GACGGATTGG ACAGCCCACA CTGCTGCTGT
421ATGTGGACGC AGGCCCTGAG ACCATGACCC AGCGGCTCTT GAAACGTGGA GAGACCAGCG
481GGCGTGTGGA CGACAATGAG GAGACCATCA AAAAGCGGCT GGAGACCTAT TACAAGGCCA
541CAGAACCCGT CATCGCCTTC TATGAGAAAC GTGGCATTGT GCGCAAGGTC AACGCTGAGG
601GCTCCGTGGA CAGTGTCTTC TCCCAGGTCT GCACCCACCT GGACGCCCTA TTGAATTGAG
661ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
721GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
781TTTATATATC TTGTGGAAAG GACGAGAACC TGCCAACTGG CAGTAGAACA CGCGTTAAGT
841Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)