Construct: ORF TRCN0000488844
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020129.1_s317c1
- DNA Barcode:
- TGGCATACTGATATCATATGTGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NME3 (4832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488844
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4832 | NME3 | NME/NM23 nucleoside diphosp... | NM_002513.2 | 99.8% | 100% | 282A>T |
| 2 | human | 4832 | NME3 | NME/NM23 nucleoside diphosp... | XM_005255332.4 | 97.4% | 75% | 282A>T;384_385insGTTGGCAA;495_496insTGAG |
| 3 | human | 4832 | NME3 | NME/NM23 nucleoside diphosp... | XM_011522503.2 | 96.1% | 58.8% | 177_187del;293A>T;395_396insGTTGGCAA |
| 4 | human | 4832 | NME3 | NME/NM23 nucleoside diphosp... | XM_011522504.2 | 76.4% | 33.3% | 177_187del;293A>T;408_409ins110 |
| 5 | mouse | 79059 | Nme3 | NME/NM23 nucleoside diphosp... | NM_019730.2 | 82.8% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 579
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatctgc ctggtgctga ccatcttcgc taacctcttc cccgcggcct 121 gcaccggcgc acacgaacgc accttcctgg ccgtgaagcc ggacggcgtg cagcggcggc 181 tggtgggcga gattgtgcgg cgcttcgaga ggaagggctt caagttggtg gcgctgaagc 241 tggtgcaggc cTCCGAGGAG CTGCTGCGTG AGCACTACGC CGAGCTGCGT GAACGCCCGT 301 TCTACGGCCG CCTTGTCAAG TATATGGCCT CCGGGCCGGT GGTGGCCATG GTTTGGCAGG 361 GGCTGGACGT GGTGCGCACC TCGCGGGCGC TCATCGGAGC CACGAACCCG GCCGACGCCC 421 CGCCCGGCAC CATCCGCGGG GATTTCTGCA TCGAGGTTGG CAAGAACCTG ATTCACGGCA 481 GCGACTCGGT GGAGAGTGCC CGCCGCGAGA TCGCTCTCTG GTTCCGCGCA GACGAGCTCC 541 TCTGCTGGGA GGACAGCGCT GGGCACTGGC TGTATGAGTG AGACCCAGCT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGATGG CATACTGATA TCATATGTGT AACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t