Construct: ORF TRCN0000488856
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021603.1_s317c1
- DNA Barcode:
- TTCTAATTCATGAGCTCAAAGTGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PDGFRL (5157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488856
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5157 | PDGFRL | platelet derived growth fac... | NM_006207.2 | 99.9% | 100% | 1041T>C |
| 2 | human | 5157 | PDGFRL | platelet derived growth fac... | NM_001372073.1 | 99.8% | 100% | 792T>C;1041T>C |
| 3 | mouse | 68797 | Pdgfrl | platelet-derived growth fac... | NM_026840.3 | 84.1% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1197
- ORF length:
- 1125
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaggtc tggctgctgc ttggtcttct gctggtgcac gaagcgctgg 121 aggatgttac tggccaacac cttcccaaga acaagcgtcc aaaagaacca ggagagaata 181 gaatcaaacc taccaacaag aaggtgaagc ccaaaattcc taaaatgaag gacagggact 241 cagccaattc agcaccaaag acgcagtcta tcatgatgca agtgctggat aaaggtcgct 301 tccagaaacc cgccgctacc ctgagtctgc tggcggggca aactgtagag cttcgatgta 361 aagggagtag aattgggtgg agctaccctg cgtatctgga cacctttaag gattctcgcc 421 tcagcgtcaa gcagaatgag cgctacggcc agttgactct ggtcaactcc acctcggcag 481 acacaggtga attcagctgc tgggtgcagc tctgcagcgg ctacatctgc aggaaggacg 541 aggccaaaac gggctccacc tacatctttt ttacagagaa aggagaactc tttgtacctt 601 ctcccagcta cttcgatgtt gtctacttga acccggacag acaggctgtg gttccttgtc 661 gggtgaccgt gctgtcggcc aaagtcacgc tccacaggga attcccagcc aaggagatcc 721 cagccaatgg aacggacatt gtttatgaca tgaagcgggg ctttgtgtat ctgcaacctc 781 attccgagca ccagggtgtg gtttactgca gggcggaggc cgggggcaga tctcagatct 841 ccgtcaagta ccagctgctc tacgtggcgg ttcccagtgg CCCTCCCTCA ACAACCATCT 901 TGGCTTCTTC AAACAAAGTG AAAAGTGGGG ACGACATCAG TGTGCTCTGC ACTGTCCTGG 961 GGGAGCCCGA TGTGGAGGTG GAGTTCACCT GGATCTTCCC AGGGCAGAAG GATGAAAGGC 1021 CTGTGACGAT CCAAGACACT TGGAGGTTGA TCCACAGAGG ACTGGGACAC ACCACGAGAA 1081 TCTCCCAGAG TGTCATTACA GTGGAAGACT TCGAGACGAT TGATGCAGGA TATTACATTT 1141 GCACTGCTCA GAATCTTCAA GGACAGACCA CAGTAGCTAC CACTGTTGAG TTTTCCTAGA 1201 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1261 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1321 TTTATATATC TTGTGGAAAG GACGATTCTA ATTCATGAGC TCAAAGTGGA CGCGTTAAGT 1381 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt