Construct: ORF TRCN0000488865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020459.1_s317c1
- DNA Barcode:
- GGTTCCGGATAACAGTCTTTCTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKCB (5579)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5579 | PRKCB | protein kinase C beta | NM_002738.7 | 99.9% | 100% | 1191T>C |
| 2 | human | 5579 | PRKCB | protein kinase C beta | NM_212535.3 | 95.3% | 95.6% | (many diffs) |
| 3 | mouse | 18751 | Prkcb | protein kinase C, beta | NM_008855.2 | 91.4% | 98.8% | (many diffs) |
| 4 | mouse | 18751 | Prkcb | protein kinase C, beta | NM_001316672.1 | 87.6% | 94.5% | (many diffs) |
| 5 | mouse | 18751 | Prkcb | protein kinase C, beta | XM_011241695.2 | 73.5% | 79.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2091
- ORF length:
- 2019
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctgac ccggctgcgg ggccgccgcc gagcgagggc gaggagagca 121 ccgtgcgctt cgcccgcaaa ggcgccctcc ggcagaagaa cgtgcatgag gtcaagaacc 181 acaaattcac cgcccgcttc ttcaagcagc ccaccttctg cagccactgc accgacttca 241 tctggggctt cgggaagcag ggattccagt gccaagtttg ctgctttgtg gtgcacaagc 301 ggtgccatga atttgtcaca ttctcctgcc ctggcgctga caagggtcca gcctccgatg 361 acccccgcag caaacacaag tttaagatcc acacgtactc cagccccacg ttttgtgacc 421 actgtgggtc actgctgtat ggactcatcc accaggggat gaaatgtgac acctgcatga 481 tgaatgtgca caagcgctgc gtgatgaatg ttcccagcct gtgtggcacg gaccacacgg 541 agcgccgcgg ccgcatctac atccaggccc acatcgacag ggacgtcctc attgtcctcg 601 taagagatgc taaaaacctt gtacctatgg accccaatgg cctgtcagat ccctacgtaa 661 aactgaaact gattcccgat cccaaaagtg agagcaaaca gaagaccaaa accatcaaat 721 gctccctcaa ccctgagtgg aatgagacat ttagatttca gctgaaagaa tcggacaaag 781 acagaagact gtcagtagag atttgggatt gggatttgac cagcaggaat gacttcatgg 841 gatctttgtc ctttgggatt tctgaacttc agaaagccag tgttgatggc tggtttaagt 901 tactgagcca ggaggaaggc gagtacttca atgtgcctgt gccaccagaa ggaagtgagg 961 ccaatgaaga actgcggcag aaatttgaga gggccaagat cagtcaggga accaaggtcc 1021 cggaagaaaa gacgaccaac actgtctcca aatttgacaa caatggcaac agagaccgga 1081 tgaaactgac cgattttaac ttcctaatgg tgctggggaa aggcagcttt ggcaaggtca 1141 tgctttcaga acgaaaaggc acagatgagc tctatgctgt gaagatcctg aagaaggacg 1201 ttgtgatcca agatgatgac gtggagtgca ctatggtgga gaagcgggtg ttggccctgc 1261 ccgggaagcc gcccttcctg acccagctcc actcctgctt ccagaccatg gaccgcctgt 1321 actttgtgat ggagtacgtg aatgggggcg acctcatgta tcacatccag caagtcggcc 1381 ggttcaagga gccccatgct gtattttacg ctgcagaaat tgccatcggt ctgttcttct 1441 tacagagtaa gggcatcatt taccgtgacc taaaacttga caacgtgatg ctcgattctg 1501 agggacacat caagattgcc gattttggca tgtgtaagga aaacatctgg gatggggtga 1561 caaccaagac attctgtggc actccagact acatcgcccc cgagataatt gcttatcagc 1621 cctatgggaa gtccgtggat tggtgggcat ttggagtcct gctgtatgaa atgttggctg 1681 ggcaggcacc ctttgaaggg gaggatgaag atgaactctt cCAATCCATC ATGGAACACA 1741 ACGTAGCCTA TCCCAAGTCT ATGTCCAAGG AAGCTGTGGC CATCTGCAAA GGGCTGATGA 1801 CCAAACACCC AGGCAAACGT CTGGGTTGTG GACCTGAAGG CGAACGTGAT ATCAAAGAGC 1861 ATGCATTTTT CCGGTATATT GATTGGGAGA AACTTGAACG CAAAGAGATC CAGCCCCCTT 1921 ATAAGCCAAA AGCTTGTGGG CGAAATGCTG AAAACTTCGA CCGATTTTTC ACCCGCCATC 1981 CACCAGTCCT AACACCTCCC GACCAGGAAG TCATCAGGAA TATTGACCAA TCAGAATTCG 2041 AAGGATTTTC CTTTGTTAAC TCTGAATTTT TAAAACCCGA AGTCAAGAGC TAGGACCCAG 2101 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2161 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2221 TATCTTGTGG AAAGGACGAG GTTCCGGATA ACAGTCTTTC TTTACGCGTT AAGTCgacaa 2281 tcaacctctg gattacaaaa tttgtgaaag att