Construct: ORF TRCN0000488869
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020192.1_s317c1
- DNA Barcode:
- TCGCCACGAGTGTCCCACGTATGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NRBP1 (29959)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488869
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321357.1 | 100% | 100% | |
| 2 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_013392.4 | 100% | 100% | |
| 3 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321362.2 | 99.8% | 99.8% | 743_744insAGA |
| 4 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321358.2 | 98.5% | 98.5% | 660_683del |
| 5 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321359.2 | 98.5% | 98.5% | 660_683del |
| 6 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321361.2 | 98.3% | 98.3% | 660_683del;926_927insGGA |
| 7 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321363.2 | 94% | 94% | 338_339ins96 |
| 8 | mouse | 192292 | Nrbp1 | nuclear receptor binding pr... | NM_147201.2 | 93.2% | 98.3% | (many diffs) |
| 9 | mouse | 192292 | Nrbp1 | nuclear receptor binding pr... | NM_001347326.1 | 91.8% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1677
- ORF length:
- 1605
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcggag ggggagtccc agacagtact tagcagtggc tcagacccaa 121 aggtagaatc ctcatcttca gctcctggcc tgacatcagt gtcacctcct gtgacctcca 181 caacctcagc tgcttcccca gaggaagaag aagaaagtga agatgagtct gagattttgg 241 aagagtcgcc ctgtgggcgc tggcagaaga ggcgagaaga ggtgaatcaa cggaatgtac 301 caggtattga cagtgcatac ctggccatgg atacagagga aggtgtagag gttgtgtgga 361 atgaggtaca gttctctgaa cgcaagaact acaagctgca ggaggaaaag gttcgtgctg 421 tgtttgataa tctgattcaa ttggagcatc ttaacattgt taagtttcac aaatattggg 481 ctgacattaa agagaacaag gccagggtca tttttatcac agaatacatg tcatctggga 541 gtctgaagca atttctgaag aagaccaaaa agaaccacaa gacgatgaat gaaaaggcat 601 ggaagcgttg gtgcacacaa atcctctctg ccctaagcta cctgcactcc tgtgaccccc 661 ccatcatcca tgggaacctg acctgtgaca ccatcttcat ccagcacaac ggactcatca 721 agattggctc tgtggctcct gacactatca acaatcatgt gaagacttgt cgagaagagc 781 agaagaatct acacttcttt gcaccagagt atggagaagt cactaatgtg acaacagcag 841 tggacatcta ctcctttggc atgtgtgcac tggagatggc agtgctggag attcagggca 901 atggagagtc ctcatatgtg ccacaggaag ccatcagcag tgccatccag cttctagaag 961 acccattaca gagggagttc attcaaaagt gcctgcagtc tgagcctgct cgcagaccaa 1021 cagccagaga acttctgttc cacccagcat tgtttgaagt gccctcgctc aaactccttg 1081 cggcccactg cattgtggga caccaacaca tgatcccaga gaacgctcta gaggagatca 1141 ccaaaaacat ggatactagt gccgtactgg ctgaaatccc tgcaggacca ggaagagaac 1201 cagttcagac tttgtactct cagtcaccag ctctggaatt agataaattc cttgaagatg 1261 tcaggaatgg gatctatcct ctgacagcct ttgggctgcc tcggccccag cagccacagc 1321 aggaggaggt gacatcacct gtcgtgcccc cctctgtcaa gactccgaca ccTGAACCAG 1381 CTGAGGTGGA GACTCGCAAG GTGGTGCTGA TGCAGTGCAA CATTGAGTCG GTGGAGGAGG 1441 GAGTCAAACA CCACCTGACA CTTCTGCTGA AGTTGGAGGA CAAACTGAAC CGGCACCTGA 1501 GCTGTGACCT GATGCCAAAT GAGAATATCC CCGAGTTGGC GGCTGAGCTG GTGCAGCTGG 1561 GCTTCATTAG TGAGGCTGAC CAGAGCCGGT TGACTTCTCT GCTAGAAGAG ACCTTGAACA 1621 AGTTCAATTT TGCCAGGAAC AGTACCCTCA ACTCAGCCGC TGTCACCGTC TCCTCTTGAG 1681 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1741 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1801 TTTATATATC TTGTGGAAAG GACGATCGCC ACGAGTGTCC CACGTATGGA CGCGTTAAGT 1861 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt