Construct: ORF TRCN0000488873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019704.1_s317c1
- DNA Barcode:
- GGAGCCTAGACGATTTGACCACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABCB1 (5243)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5243 | ABCB1 | ATP binding cassette subfam... | NM_000927.4 | 99.9% | 99.9% | 3840_3841insG |
| 2 | human | 5243 | ABCB1 | ATP binding cassette subfam... | NM_001348944.1 | 99.9% | 99.9% | 3840_3841insG |
| 3 | human | 5243 | ABCB1 | ATP binding cassette subfam... | NM_001348946.2 | 99.9% | 99.9% | 3840_3841insG |
| 4 | human | 5243 | ABCB1 | ATP binding cassette subfam... | NM_001348945.1 | 94.7% | 94.7% | 1_210del;4050_4051insG |
| 5 | mouse | 18671 | Abcb1a | ATP-binding cassette, sub-f... | NM_011076.2 | 85.7% | 87.1% | (many diffs) |
| 6 | mouse | 18671 | Abcb1a | ATP-binding cassette, sub-f... | XM_006503555.3 | 85.7% | 87.1% | (many diffs) |
| 7 | mouse | 18671 | Abcb1a | ATP-binding cassette, sub-f... | XM_006503556.3 | 85.7% | 87.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3918
- ORF length:
- 3843
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggat cttgaagggg accgcaatgg aggagcaaag aagaagaact 121 tttttaaact gaacaataaa agtgaaaaag ataagaagga aaagaaacca actgtcagtg 181 tattttcaat gtttcgctat tcaaattggc ttgacaagtt gtatatggtg gtgggaactt 241 tggctgccat catccatggg gctggacttc ctctcatgat gctggtgttt ggagaaatga 301 cagatatctt tgcaaatgca ggaaatttag aagatctgat gtcaaacatc actaatagaa 361 gtgatatcaa tgatacaggg ttcttcatga atctggagga agacatgacc aggtatgcct 421 attattacag tggaattggt gctggggtgc tggttgctgc ttacattcag gtttcatttt 481 ggtgcctggc agctggaaga caaatacaca aaattagaaa acagtttttt catgctataa 541 tgcgacagga gataggctgg tttgatgtgc acgatgttgg ggagcttaac acccgactta 601 cagatgatgt ctccaagatt aatgaaggaa ttggtgacaa aattggaatg ttctttcagt 661 caatggcaac atttttcact gggtttatag taggatttac acgtggttgg aagctaaccc 721 ttgtgatttt ggccatcagt cctgttcttg gactgtcagc tgctgtctgg gcaaagatac 781 tatcttcatt tactgataaa gaactcttag cgtatgcaaa agctggagca gtagctgaag 841 aggtcttggc agcaattaga actgtgattg catttggagg acaaaagaaa gaacttgaaa 901 ggtacaacaa aaatttagaa gaagctaaaa gaattgggat aaagaaagct attacagcca 961 atatttctat aggtgctgct ttcctgctga tctatgcatc ttatgctctg gccttctggt 1021 atgggaccac cttggtcctc tcaggggaat attctattgg acaagtactc actgtattct 1081 tttctgtatt aattggggct tttagtgttg gacaggcatc tccaagcatt gaagcatttg 1141 caaatgcaag aggagcagct tatgaaatct tcaagataat tgataataag ccaagtattg 1201 acagctattc gaagagtggg cacaaaccag ataatattaa gggaaatttg gaattcagaa 1261 atgttcactt cagttaccca tctcgaaaag aagttaagat cttgaagggt ctgaacctga 1321 aggtgcagag tgggcagacg gtggccctgg ttggaaacag tggctgtggg aagagcacaa 1381 cagtccagct gatgcagagg ctctatgacc ccacagaggg gatggtcagt gttgatggac 1441 aggatattag gaccataaat gtaaggtttc tacgggaaat cattggtgtg gtgagtcagg 1501 aacctgtatt gtttgccacc acgatagctg aaaacattcg ctatggccgt gaaaatgtca 1561 ccatggatga gattgagaaa gctgtcaagg aagccaatgc ctatgacttt atcatgaaac 1621 tgcctcataa atttgacacc ctggttggag agagaggggc ccagttgagt ggtgggcaga 1681 agcagaggat cgccattgca cgtgccctgg ttcgcaaccc caagatcctc ctgctggatg 1741 aggccacgtc agccttggac acagaaagcg aagcagtggt tcaggtggct ctggataagg 1801 ccagaaaagg tcggaccacc attgtgatag ctcatcgttt gtctacagtt cgtaatgctg 1861 acgtcatcgc tggtttcgat gatggagtca ttgtggagaa aggaaatcat gatgaactca 1921 tgaaagagaa aggcatttac ttcaaacttg tcacaatgca gacagcagga aatgaagttg 1981 aattagaaaa tgcagctgat gaatccaaaa gtgaaattga tgccttggaa atgtcttcaa 2041 atgattcaag atccagtcta ataagaaaaa gatcaactcg taggagtgtc cgtggatcac 2101 aagcccaaga cagaaagctt agtaccaaag aggctctgga tgaaagtata cctccagttt 2161 ccttttggag gattatgaag ctaaatttaa ctgaatggcc ttattttgtt gttggtgtat 2221 tttgtgccat tataaatgga ggcctgcaac cagcatttgc aataatattt tcaaagatta 2281 taggggtttt tacaagaatt gatgatcctg aaacaaaacg acagaatagt aacttgtttt 2341 cactattgtt tctagccctt ggaattattt cttttattac atttttcctt cagggtttca 2401 catttggcaa agctggagag atcctcacca agcggctccg atacatggtt ttccgatcca 2461 tgctcagaca ggatgtgagt tggtttgatg accctaaaaa caccactgga gcattgacta 2521 ccaggctcgc caatgatgct gctcaagtta aaggggctat aggttccagg cttgctgtaa 2581 ttacccagaa tatagcaaat cttgggacag gaataattat atccttcatc tatggttggc 2641 aactaacact gttactctta gcaattgtac ccatcattgc aatagcagga gttgttgaaa 2701 tgaaaatgtt gtctggacaa gcactgaaag ataagaaaga actagaaggt tctgggaaga 2761 tcgctactga agcaatagaa aacttccgaa ccgttgtttc tttgactcag gagcagaagt 2821 ttgaacatat gtatgctcag agtttgcagg taccatacag aaactctttg aggaaagcac 2881 acatctttgg aattacattt tccttcaccc aggcaatgat gtatttttcc tatgctggat 2941 gtttccggtt tggagcctac ttggtggcac ataaactcat gagctttgag gatgttctgt 3001 tagtattttc agctgttgtc tttggtgcca tggccgtggg gcaagtcagt tcatttgctc 3061 ctgactatgc caaagccaaa atatcagcag cccacatcat catgatcatt gaaaaaaccc 3121 ctttgattga cagctacagc acggaaggcc taatgccgaa cacattggaa ggaaatgtca 3181 catttggtga agttgtattc aactatccca cccgaccgga catcccagtg cttcagggac 3241 tgagcctgga ggtgaagaag ggccagacgc tggctctggt gggcagcagt ggctgtggga 3301 agagcacagt ggtccagctc ctggagcggt tctacgaccc cttggcaggg aaagtgctgc 3361 ttgatggcaa agaaataaag cgactgaatg ttcagtggct ccgagcacac ctgggcatcg 3421 tgtcccagga gcccatcctg tttgactgca gcattgctga gaacattgcc tatggagaca 3481 acagccgggt ggtgtcacag gaagagattg tgagggcagc aaaggaggcc aacatacatg 3541 ccttcatcga gtcactgcct aataaatata gcactaaagt aggagacaaa ggaacTCAGC 3601 TCTCTGGTGG CCAGAAACAA CGCATTGCCA TAGCTCGTGC CCTTGTTAGA CAGCCTCATA 3661 TTTTGCTTTT GGATGAAGCC ACGTCAGCTC TGGATACAGA AAGTGAAAAG GTTGTCCAAG 3721 AAGCCCTGGA CAAAGCCAGA GAAGGCCGCA CCTGCATTGT GATTGCTCAC CGCCTGTCCA 3781 CCATCCAGAA TGCAGACTTA ATAGTGGTGT TTCAGAATGG CAGAGTCAAG GAGCATGGCA 3841 CGCATCAGCA GCTGCTGGCA CAGAAAGGCA TCTATTTTTC AATGGTCAGT GTCCAGGCTG 3901 GAACAAAGCG CCAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3961 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGCCCGTAA CTTGAAAGTA 4021 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGAGCCTAGA CGATTTGACC 4081 ACTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt