Construct: ORF TRCN0000488890
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021292.1_s317c1
- DNA Barcode:
- ACGCTGCTCGCCTAACGTAACAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PPIL3 (53938)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488890
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | NM_130906.3 | 99.7% | 99.3% | 438T>G |
| 2 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_005246652.5 | 99.7% | 99.3% | 438T>G |
| 3 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_011511358.2 | 99.7% | 99.3% | 438T>G |
| 4 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004355.2 | 99.7% | 99.3% | 438T>G |
| 5 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004356.2 | 99.7% | 99.3% | 438T>G |
| 6 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | NM_032472.4 | 88% | 74.8% | (many diffs) |
| 7 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_005246651.3 | 88% | 74.8% | (many diffs) |
| 8 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004352.1 | 88% | 74.8% | (many diffs) |
| 9 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004353.1 | 88% | 74.8% | (many diffs) |
| 10 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004354.2 | 88% | 74.8% | (many diffs) |
| 11 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004357.1 | 69.3% | 68.9% | 0_1ins147;291T>G |
| 12 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004358.1 | 69.3% | 68.9% | 0_1ins147;291T>G |
| 13 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004359.1 | 69.3% | 68.9% | 0_1ins147;291T>G |
| 14 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004360.1 | 69.3% | 68.9% | 0_1ins147;291T>G |
| 15 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | NM_001285826.1 | 93.1% | 97.5% | (many diffs) |
| 16 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | NM_001285827.1 | 93.1% | 97.5% | (many diffs) |
| 17 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | NM_027351.3 | 93.1% | 97.5% | (many diffs) |
| 18 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238590.2 | 74.9% | 61.3% | (many diffs) |
| 19 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238591.2 | 74.9% | 61.3% | (many diffs) |
| 20 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238592.2 | 74.9% | 61.3% | (many diffs) |
| 21 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238593.2 | 74.9% | 61.3% | (many diffs) |
| 22 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238594.2 | 74.9% | 61.3% | (many diffs) |
| 23 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238595.2 | 74.9% | 61.3% | (many diffs) |
| 24 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | NM_027374.3 | 68.9% | 72.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 555
- ORF length:
- 483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctgtg acactgcata cagatgtagg tgatattaaa attgaagtct 121 tctgtgagag gacacccaaa acatgtgaga atttcttggc tctttgtgcc agtaattact 181 acaatGGCTG TATATTTCAT AGGAATATCA AGGGTTTCAT GGTTCAAACA GGAGATCCAA 241 CAGGAACTGG AAGAGGAGGC AACAGTATTT GGGGCAAGAA GTTTGAGGAT GAATACAGTG 301 AATATCTTAA GCACAATGTT AGAGGTGTTG TATCTATGGC TAATAATGGC CCGAACACCA 361 ATGGATCTCA GTTCTTCATC ACCTATGGCA AACAGCCACA TTTGGACATG AAATACACCG 421 TATTTGGAAA GGTAATAGAT GGTCTGGAAA CTCTAGATGA GTTGGAGAAG TTGCCAGTAA 481 ATGAGAAGAC ATACCGACCT CTTAATGAGG TACACATTAA GGACATAACT ATTCATGCCA 541 ACCCATTTGC TCAGTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACGCTGC TCGCCTAACG 721 TAACAACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt