Construct: ORF TRCN0000488899
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020588.1_s317c1
- DNA Barcode:
- GACAAGATGAGCCTCATCCCAAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K3 (4215)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488899
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4215 | MAP3K3 | mitogen-activated protein k... | NM_001330431.2 | 100% | 100% | |
| 2 | human | 4215 | MAP3K3 | mitogen-activated protein k... | NM_002401.5 | 99.3% | 99.3% | 694_705del |
| 3 | human | 4215 | MAP3K3 | mitogen-activated protein k... | NM_001363768.2 | 95.2% | 95.2% | 126_218del |
| 4 | human | 4215 | MAP3K3 | mitogen-activated protein k... | NM_203351.3 | 94.6% | 94.6% | 126_218del;787_798del |
| 5 | human | 4215 | MAP3K3 | mitogen-activated protein k... | XM_005257378.2 | 75.6% | 73.5% | (many diffs) |
| 6 | human | 4215 | MAP3K3 | mitogen-activated protein k... | XR_934463.1 | 60.3% | (many diffs) | |
| 7 | mouse | 26406 | Map3k3 | mitogen-activated protein k... | NM_011947.3 | 89.8% | 96% | (many diffs) |
| 8 | mouse | 26406 | Map3k3 | mitogen-activated protein k... | XM_011249037.3 | 88.5% | 94.5% | (many diffs) |
| 9 | mouse | 26406 | Map3k3 | mitogen-activated protein k... | XM_030246020.1 | 88.5% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1938
- ORF length:
- 1866
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacgaa caggaggcat tgaactcaat catgaacgat ctggtggccc 121 tccagatgaa ccgacgtcac cggatgcctg gatatgagac catgaagaac aaagacacag 181 gtcactcaaa taggcagagt gacgtcagaa tcaagttcga gcacaacggg gagaggcgaa 241 ttatagcgtt cagccggcct gtgaaatatg aagatgtgga gcacaaggtg acaacagtat 301 ttggacaacc tcttgatcta cattacatga acaatgagct ctccatcctg ctgaaaaacc 361 aagatgatct tgataaagca attgacattt tagatagaag ctcaagcatg aaaagcctta 421 ggatattgct gttgtcccag gacagaaacc ataacagttc ctctccccac tctggggtgt 481 ccagacaggt gcggatcaag gcttcccagt ccgcagggga tataaatact atctaccagc 541 cccccgagcc cagaagcagg cacctctctg tcagctccca gaaccctggc cgaagctcac 601 ctccccctgg ctatgttcct gagcggcagc agcacattgc ccggcagggg tcctacacca 661 gcatcaacag tgagggggag ttcatcccag agaccagcga gcagtgcatg ctggatcccc 721 tgagcagtgc agaaaattcc ttgtctggaa gctgccaatc cttggacagc ccatccttcc 781 ggaaatcacg aatgtcccgt gcccagagct tccctgacaa cagacaggaa tactcagatc 841 gggaaactca gctttatgac aaaggggtca aaggtggaac ctacccccgg cgctaccacg 901 tgtctgtgca ccacaaggac tacagtgatg gcagaagaac atttccccga atacggcgtc 961 atcaaggcaa cttgttcacc ctggtgccct ccagccgctc cctgagcaca aatggcgaga 1021 acatgggtct ggctgtgcaa tacctggacc cccgtgggcg cctgcggagt gcggacagcg 1081 agaatgccct ctctgtgcag gagaggaatg tgccaaccaa gtctcccagt gcccccatca 1141 actggcgccg gggaaagctc ctgggccagg gtgccttcgg cagggtctat ttgtgctatg 1201 acgtggacac gggacgtgaa cttgcttcca agcaggtcca atttgatcca gacagtcctg 1261 agacaagcaa ggaggtgagt gctctggagt gcgagatcca gttgctaaag aacttgcagc 1321 atgagcgcat cgtgcagtac tatggctgtc tgcgggaccg cgctgagaag accctgacca 1381 tcttcatgga gtacatgcca gggggctcgg tgaaagacca gttgaaggct tacggtgctc 1441 tgacagagag cgtgacccga aagtacacgc ggcagatcct ggagggcatg tcctacctgc 1501 acagcaacat gattgttcac cgggacatta agggagccaa catcctccga gactctgctg 1561 ggaatgtaaa gctgggggac tttggggcca gcaaacgcct gcagacgatc tgtatgtcgg 1621 ggacgggcat gcgctccgtc actggcacac ccTACTGGAT GAGCCCTGAG GTGATCAGCG 1681 GCGAGGGCTA TGGAAGGAAA GCAGACGTGT GGAGCCTGGG CTGCACTGTG GTGGAGATGC 1741 TGACAGAGAA ACCACCGTGG GCAGAGTATG AAGCTATGGC CGCCATCTTC AAGATTGCCA 1801 CCCAGCCCAC CAATCCTCAG CTGCCCTCCC ACATCTCTGA ACATGGCCGG GACTTCCTGA 1861 GGCGCATTTT TGTGGAGGCT CGCCAGAGAC CTTCAGCTGA GGAGCTGCTC ACACACCACT 1921 TTGCACAGCT CATGTACTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1981 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGC CCGTAACTTG 2041 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGGGAAAG GACGAGACAA GATGAGCCTC 2101 ATCCCAAGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt