Construct: ORF TRCN0000488900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020516.1_s317c1
- DNA Barcode:
- TGCGGGCCGGCCCTCCGTCAATTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SCYL1 (57410)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_017018033.1 | 61.3% | 56.9% | (many diffs) |
| 2 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_024448620.1 | 60.3% | 56.1% | (many diffs) |
| 3 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_005274120.4 | 54.4% | 54.4% | 1_1068del |
| 4 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_005274121.4 | 54.2% | 54.2% | 1_1068del;1957_1958insAGC |
| 5 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | NM_020680.4 | 52.5% | 49% | 1_1068del;2248_2302del;2399_2424del |
| 6 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_005274118.4 | 52.4% | 48.8% | (many diffs) |
| 7 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_017018031.2 | 52.2% | 52.2% | 1_1068del;1814_1815ins51 |
| 8 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | NM_001048218.2 | 50.4% | 46.9% | (many diffs) |
| 9 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_017018030.2 | 50.3% | 46.8% | (many diffs) |
| 10 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_024448619.1 | 50.1% | 46.7% | (many diffs) |
| 11 | mouse | 78891 | Scyl1 | SCY1-like 1 (S. cerevisiae) | NM_023912.2 | 44.5% | 43.2% | (many diffs) |
| 12 | mouse | 78891 | Scyl1 | SCY1-like 1 (S. cerevisiae) | XM_006531856.2 | 44.4% | 43% | (many diffs) |
| 13 | mouse | 78891 | Scyl1 | SCY1-like 1 (S. cerevisiae) | XM_006531857.2 | 43.1% | 41.9% | (many diffs) |
| 14 | mouse | 78891 | Scyl1 | SCY1-like 1 (S. cerevisiae) | XR_388273.2 | 22.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1347
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgttctca tccactgacc gggccatgcg catccgcctc ctgcagcaga 121 tggagcagtt catccagtac cttgacgagc caacagtcaa cacccagatc ttcccccacg 181 tcgtacatgg cttcctggac accaaccctg ccatccggga gcagacggtc aagtccatgc 241 tgctcctggc cccaaagctg aacgaggcca acctcaatgt ggagctgatg aagcactttg 301 cacggctaca ggccaaggat gaacagggcc ccatccgctg caacaccaca gtctgcctgg 361 gcaaaatcgg ctcctacctc agtgctagca ccagacacag ggtccttacc tctgccttca 421 gccgagccac tagggacccg tttgcaccgt cccgggttgc gggtgtcctg ggctttgctg 481 ccacccacaa cctctactca atgaacgact gtgcccagaa gatcctgcct gtgctctgcg 541 gtctcactgt agatcctgag aaatccgtgc gagaccaggc cttcaaggcc attcggagct 601 tcctgtccaa attggagtct gtgtcggagg acccgaccca gctggaggaa gtggagaagg 661 atgtccatgc agcctccagc cctggcatgg gaggagccgc agctagctgg gcaggctggg 721 ccgtgaccgg ggtctcctca ctcacctcca agctgatccg ttcgcaccca accactgccc 781 caacagaaac caacattccc caaagaccca cgcctgaagg agttcctgcc ccagccccca 841 cccctgttcc tgccacccct acaacctcag gccactggga gacgcaggag gaggacaagg 901 acacagcaga ggacagcagc actgctgaca gatgggacga cgaagactgg ggcagcctgg 961 agcaggaggc cgagtctgtg ctggcccagc aggacgactG GAGCACCGGG GGCCAAGTGA 1021 GCCGTGCTAG TCAGGTCAGC AACTCCGACC ACAAATCCTC CAAATCCCCA GAGTCCGACT 1081 GGAGCAGCTG GGAAGCTGAG GGCTCCTGGG AACAGGGCTG GCAGGAGCCA AGCTCCCAGG 1141 AGCCACCTCC TGACGGTACA CGGCTGGCCA GCGAGTATAA CTGGGGTGGC CCAGAGTCCA 1201 GCGACAAGGG CGACCCCTTC GCTACCCTGT CTGCACGTCC CAGCACCCAG GACAGGTCAA 1261 GGCTGAGCTG GCCCGGAAGA AGCGCGAGGA GCGGCGGCGG GAGATGGAGG CCAAACGCGC 1321 CGAGAGGAAG GTGGCCAAGG GCCCCATAGA ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGCGG 1501 GCCGGCCCTC CGTCAATTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt