Construct: ORF TRCN0000488924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021711.1_s317c1
- DNA Barcode:
- GGGCAGAAACGGTTTTATAGGCCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EPHA5 (2044)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488924
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2044 | EPHA5 | EPH receptor A5 | XM_017007880.2 | 49.1% | .7% | 1_1298del;1300A>T |
| 2 | human | 2044 | EPHA5 | EPH receptor A5 | XM_005265653.4 | 47.9% | .6% | 1_1364del |
| 3 | human | 2044 | EPHA5 | EPH receptor A5 | XM_017007878.2 | 46.3% | .6% | 1_1454del;1456A>T |
| 4 | human | 2044 | EPHA5 | EPH receptor A5 | XM_011531735.3 | 45.2% | .6% | 1_1520del |
| 5 | human | 2044 | EPHA5 | EPH receptor A5 | XM_017007881.2 | 45.2% | .7% | 1_1295del;1297A>T;2451_2452ins102 |
| 6 | human | 2044 | EPHA5 | EPH receptor A5 | NM_001318761.1 | 43.2% | .6% | 1_1649del |
| 7 | human | 2044 | EPHA5 | EPH receptor A5 | XM_017007879.2 | 42.6% | .6% | 1_1451del;1453A>T;2607_2608ins102 |
| 8 | human | 2044 | EPHA5 | EPH receptor A5 | NM_182472.5 | 41.2% | .5% | 1_1787del;1789A>T |
| 9 | human | 2044 | EPHA5 | EPH receptor A5 | NM_001281766.2 | 41.2% | .5% | 1_1790del;1792A>T |
| 10 | human | 2044 | EPHA5 | EPH receptor A5 | NM_004439.8 | 40.4% | .5% | 1_1853del |
| 11 | human | 2044 | EPHA5 | EPH receptor A5 | NM_001281765.2 | 40.3% | .5% | 1_1856del |
| 12 | human | 2044 | EPHA5 | EPH receptor A5 | NM_001281767.3 | 37.1% | .5% | 1_1856del;3012_3013ins102 |
| 13 | mouse | 13839 | Epha5 | Eph receptor A5 | XM_006534775.2 | 44.9% | .7% | (many diffs) |
| 14 | mouse | 13839 | Epha5 | Eph receptor A5 | NM_007937.3 | 43.9% | .6% | (many diffs) |
| 15 | mouse | 13839 | Epha5 | Eph receptor A5 | XM_006534773.3 | 41.4% | .6% | (many diffs) |
| 16 | mouse | 13839 | Epha5 | Eph receptor A5 | XM_017320657.1 | 38.9% | .6% | (many diffs) |
| 17 | mouse | 13839 | Epha5 | Eph receptor A5 | XM_006534771.3 | 37.7% | .5% | (many diffs) |
| 18 | mouse | 13839 | Epha5 | Eph receptor A5 | XM_006534770.3 | 37.3% | .5% | (many diffs) |
| 19 | mouse | 13839 | Epha5 | Eph receptor A5 | XM_006534769.2 | 36.9% | .5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 89
- ORF end:
- 149
- ORF length:
- 60
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctctgt tgcccatcca agcctaatat ggcggtgtgg ctacagcaaa gcaaaacaag 121 atccagaaga ggaaaagatg cattttcata atgggcacat taaactgcca ggagtaagaa 181 cttacattga tccacatacc tatgaggatc ccaatcaagc tgtccacgaa tttgctaagg 241 agatagaagc atcatgtatc accattgaga gagttattgg agcaggtgaa tttggtgaag 301 tttgtagtgg acgtttgaaa ctaccaggaa aaagagaatt acctgtggct atcaaaaccc 361 ttaaagtagg ctatactgaa aagcaacgca gagatttcct aggtgaagca agtatcatgg 421 gacagtttga tcatcctaac atcatccatt tagaaggtgt ggtgaccaaa agtaaaccag 481 tgatgatcgt gacagagtat atggagaatg gctctttaga tacatttttg aagaaaaacg 541 atgggcagtt cactgtgatt cagcttgttg gcatgctgag aggtatctct gcaggaatga 601 agtacctttc tgacatgggc tatgtgcata gagatcttgc tgccagaaac atcttaatca 661 acagtaacct tgtgtgcaaa gtgtctgact ttggactttc ccgggtactg gaagatgatc 721 ccgaggcagc ctacaccaca aggggaggaa aaattccaat cagatggact gccccagaag 781 caatagcttt ccgaaagttt acttctgcca gtgatgtctg gagttatgga atagtaatgt 841 gggaagttgt gtcttatgga gagagaccct actgggagat gaccaatcaa gatgtgatta 901 aagcggtaga ggaaggctat cgtctgccaa gccccatgga ttgtcctgct gctctctatc 961 agttaatgct ggattgctgg CAGAAAGAGC GAAATAGCAG GCCCAAGTTT GATGAAATAG 1021 TCAACATGTT GGACAAGCTG ATACGTAACC CAAGTAGTCT GAAGACGCTG GTTAATGCAT 1081 CCTGCAGAGT ATCTAATTTA TTGGCAGAAC ATAGCCCACT AGGATCTGGG GCCTACAGAT 1141 CAGTAGGTGA ATGGCTAGAG GCAATCAAGA TGGGCCGGTA TACAGAGATT TTCATGGAAA 1201 ATGGATACAG TTCAATGGAC GCTGTGGCTC AGGTGACCTT GGAGGATTTG AGACGGCTTG 1261 GAGTGACTCT TGTCGGTCAC CAGAAGAAGA TCATGAACAG CCTTCAAGAA ATGAAGGTGC 1321 AGCTGGTAAA CGGAATGGTG CCATTGTAAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGGCA 1501 GAAACGGTTT TATAGGCCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt