Transcript: Mouse XM_017320657.1

PREDICTED: Mus musculus Eph receptor A5 (Epha5), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Epha5 (13839)
Length:
3815
CDS:
807..3425

Additional Resources:

NCBI RefSeq record:
XM_017320657.1
NBCI Gene record:
Epha5 (13839)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017320657.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000368778 TCTGTCCGTGTCTACTATAAA pLKO_005 1485 CDS 100% 15.000 21.000 N Epha5 n/a
2 TRCN0000023328 CCTATATTGATCCGCACACTT pLKO.1 2359 CDS 100% 4.950 6.930 N Epha5 n/a
3 TRCN0000023327 GCTGGACAAACTGATACGAAA pLKO.1 3206 CDS 100% 4.950 6.930 N Epha5 n/a
4 TRCN0000023324 GCACTTTCATAACGGGCACAT pLKO.1 2318 CDS 100% 4.050 3.240 N Epha5 n/a
5 TRCN0000361188 AGTCTAAAGAGACCAGTATTA pLKO_005 2032 CDS 100% 13.200 9.240 N Epha5 n/a
6 TRCN0000023326 GCAGATTCATCACAGTTGTTA pLKO.1 1560 CDS 100% 5.625 3.938 N Epha5 n/a
7 TRCN0000023325 CCTACAGATCAGTAGGTGAAT pLKO.1 3310 CDS 100% 0.495 0.347 N Epha5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017320657.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488924 GGGCAGAAACGGTTTTATAGGCCT pLX_317 29.1% 38.9% .6% V5 (not translated due to prior stop codon) (many diffs) n/a
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