Construct: ORF TRCN0000488930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021376.1_s317c1
- DNA Barcode:
- CTCCCAAGGCGTAGTCAGACACGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PTPRN2 (5799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | NM_002847.5 | 99.7% | 99.8% | (many diffs) |
| 2 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | NM_130842.4 | 98% | 98.1% | (many diffs) |
| 3 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | NM_130843.4 | 96.8% | 96.9% | (many diffs) |
| 4 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | NM_001308267.2 | 95.9% | 96% | (many diffs) |
| 5 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | NM_001308268.1 | 95.5% | 94.6% | (many diffs) |
| 6 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | XM_017012475.1 | 93.2% | 93.3% | (many diffs) |
| 7 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | XM_017012476.1 | 83.4% | 81.8% | (many diffs) |
| 8 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | XM_011516446.1 | 81.9% | 81.7% | (many diffs) |
| 9 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | XM_011516447.2 | 64.9% | 58.9% | (many diffs) |
| 10 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | XM_011516448.3 | 61.3% | 57.7% | (many diffs) |
| 11 | human | 5799 | PTPRN2 | protein tyrosine phosphatas... | XM_011516449.2 | 56.1% | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3111
- ORF length:
- 3045
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatggg gccgccgctc ccgctgctgc tgctgctact gctgctgctg ccgccacgcg 121 tcctgcctgc cgccccttcg tccgtccccc gcggccggca gctcccgggg cgtctgggct 181 gcctgctcga ggagggcctc tgcggagcgt ccgaggcctg tgtgaacgat ggagtgtttg 241 gaaggtgcca gaaggttccg gcaatggact tttaccgcta cgaggtgtcg cccgtggccc 301 tgcagcgcct gcgcgtggcg ttgcagaagc tttccggcac aggtttcacg tggcaggatg 361 actatactca gtatgtgatg gaccaggaac ttgcagacct cccgaaaacc tacctgaggc 421 gtcctgaagc atccagccca gccaggccct caaaacacag cgttggcagc gagaggaggt 481 acagtcggga gggcggtgct gccctggcca acgccctccg acgccacctg cccttcctgg 541 aggccctgtc ccaggcccca gcctcagacg tgctcgccag gacccatacg gcgcaggaca 601 gaccccccgc tgagggtgat gaccgcttct ccgagagcat cctgacctat gtggcccaca 661 cgtctgcgct gacctaccct cccgggcccc ggacccagct ccgcgaggac ctcctgccgc 721 ggaccctcgg ccagctccag ccagatgagc tcagccctaa ggtggacagt ggtgtggaca 781 gacaccatct gatggcggcc ctcagtgcct atgctgccca gaggccccca gctccccccg 841 gggagggcag cctggagcca cagtaccttc tgcgtgcacc ctcaagaatg cccaggcctt 901 tgctggcacc agccgccccc cagaagtggc cttcacctct gggagattcc gaagacccct 961 ctagcacagg cgatggagca cggattcata ccctcctgaa ggacctgcag aggcagccgg 1021 ctgaggtgag gggcctgaat ggcctggagc tggacggcat ggccgagctg atggctggcc 1081 tgatgcaagg cgtggaccat ggagtagctc gaggcagccc tgggagagcg gccctgggag 1141 agtctggaga acaggcggat ggccccaagg ccaccctccg tggagacagc tttccagatg 1201 acggagtgca ggacgacgat gatagacttt accaagaggt ccatcgtctg agtgccacac 1261 tcgggggcct cctgcaggac cacgggtctc gactcttacc tggagccctc ccctttgcaa 1321 ggcccctcga catggagagg aagaagtccg agcaccctga gtcttccctg tcttcagaag 1381 aggagactgc cggagtggag aacgtcaaga gccagacgta ttccaaagat ctgctggggc 1441 agcagccgca ttcggagccc ggggccgctg cgtttgggga gctccaaaac cagatgcctg 1501 ggccctcgaa ggaggagcag agccttccag cgggtgctca ggaggccctc agcgacggcc 1561 tgcaattgga ggtccagcct tccgaggaag aggcgcgggg ctacatcgtg acagacagag 1621 accccctgcg ccccgaggaa ggaaggcggc tggtggagga cgtcgcccgc ctcctgcagg 1681 tgcccagcag tgcgttcgct gacgtggagg ttctcggacc agcagtgacc ttcaaagtga 1741 gcgccaatgt ccaaaacgtg accactgagg atgtggagaa ggccacagtt gacaacaaag 1801 acaaactgga ggaaacctct ggactgaaaa ttcttcaaac cggagtcggg tcgaaaagca 1861 aactcaagtt cctgcctcct caggcggagc aagaagactc caccaagttc atcgcgctca 1921 ccctggtctc cctcgcctgc atcctgggcg tcctcctggc ctctggcctc atctactgcc 1981 tccgccatag ctctcagcac aggctgaagg agaagctctc gggactaggg ggcgacccag 2041 gtgcagatgc cactgccgcc taccaggagc tgtgccgcca gcgtatggcc acgcggccac 2101 cagaccgacc tgagggcccg cacacgtcac gcatcagcag cgtctcatcc cagttcagcg 2161 acgggccgat ccccagcccc tccgcacgca gcagcgcctc atcctggtcc gaggagcctg 2221 tgcagtccaa catggacatc tccaccggcc acatgatcct gtcctacatg gaggaccacc 2281 tgaagaacaa gaaccggctg gagaaggagt gggaagcgct gtgcgcctac caggcggagc 2341 ccaacagctc gttcgtggcc cagagggagg agaacgtgcc caagaaccgc tccctggccg 2401 tgctgaccta tgaccactcc cgggtcctgc tgaaggcgga gaacagccac agccactcag 2461 actacatcaa cgctagcccc atcatggatc acgacccgag gaaccccgcg tacatcgcca 2521 cccagggacc gctgcccgcc accgtggctg acttttggca gatggtgtgg gagagcggct 2581 gcgtggtgat cgtcatgctg acacccctcg cggagaacgg cgtccggcag tgctaccact 2641 actggccgga tgaaggctcc aatctctacc acatctatga ggtgaacctg gtctccgagc 2701 acatctggtg tgaggacttc ctggtgagga gcttctatct gaagaacctg cagaccaacg 2761 agacgcgcac cgtgacgcag ttccacttcc tGAGTTGGTA TGACCGAGGA GTCCCTTCCT 2821 CCTCAAGGTC CCTCCTGGAC TTCCGCAGAA AAGTAAACAA GTGCTACAGG GGCCGTTCTT 2881 GTCCAATAAT TGTTCATTGC AGTGACGGTG CAGGCCGGAG CGGCACCTAC GTCCTGATCG 2941 ACATGGTTCT CAACAAGATG GCCAAAGGTG CTAAAGAGAT TGATATCGCA GCGACCCTGG 3001 AGCACTTGAG GGACCAGAGA CCCGGCATGG TCCAGACGAA GGAGCAGTTT GAGTTCGCGC 3061 TGACAGCCGT GGCTGAGGAG GTGAACGCCA TCCTCAAGGC CCTTCCCCAG TGAACTAGTG 3121 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3181 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3241 TTTATATATC TTGTGGAAAG GACGACTCCC AAGGCGTAGT CAGACACGAA CGCGTTAAGT 3301 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt