Construct: ORF TRCN0000488931
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021316.1_s317c1
- DNA Barcode:
- TTCGGCTCCACCTACGGTCTATAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PPWD1 (23398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488931
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | NM_015342.4 | 99.9% | 100% | 1725A>G |
| 2 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | NM_001278926.1 | 91.6% | 90.5% | 0_1ins127;68_104del;1635A>G |
| 3 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | XM_006714583.4 | 84.7% | 84.5% | (many diffs) |
| 4 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | NM_001278927.2 | 78.2% | 72.6% | 0_1ins247;77_78ins173;1305A>G |
| 5 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | NM_001278929.1 | 75.7% | 75.8% | 0_1ins468;1257A>G |
| 6 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | XM_005248474.4 | 52% | 49.3% | (many diffs) |
| 7 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | XM_017009286.1 | 49.9% | 50% | 0_1ins969;756A>G |
| 8 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | NM_172807.4 | 90.5% | 97.2% | (many diffs) |
| 9 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_006517642.3 | 84.2% | 88.1% | (many diffs) |
| 10 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XR_001780782.1 | 77.3% | (many diffs) | |
| 11 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_011244658.2 | 71.6% | 74.3% | (many diffs) |
| 12 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_017315513.1 | 69.3% | 74.6% | (many diffs) |
| 13 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XR_873754.2 | 51.6% | (many diffs) | |
| 14 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_017315514.1 | 45.7% | 49.2% | (many diffs) |
| 15 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_017315515.1 | 45.7% | 49.2% | (many diffs) |
| 16 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XR_873753.1 | 44.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 59
- ORF end:
- 1997
- ORF length:
- 1938
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccat 61 ggcggcggaa agtggtagcg attttcagca gagacgtaga aggcgccggg acccggagga 121 accggaaaaa acagaactca gcgaaagaga gctggcagta gcagtggcgg tgtcccagga 181 gaacgatgag gagaacgaag agcgctgggt tggaccttta cctgtggagg caacactggc 241 caagaagagg aaagtcttag agtttgaaag agtctatctt gataatctcc ccagtgcatc 301 catgtatgag cgcagttaca tgcatagaga tgttatcacc catgtggtat gcaccaaaac 361 agattttatt attactgcca gtcatgatgg acatgtcaag ttctggaaaa aaatagaaga 421 gggaattgaa tttgttaaac attttcgtag tcacctggga gttattgaga gtattgcagt 481 tagctctgag ggagcattgt tctgttctgt gggtgatgat aaagcaatga aggtgtttga 541 tgtagtgaac tttgacatga tcaacatgct gaaacttggc tattttcctg gacagtgtga 601 gtggatctat tgcccagggg atgcaatttc ttcagttgct gcttccgaaa agagtacagg 661 aaaaattttc atttatgatg gccgaggaga taaccagcca cttcatattt ttgacaaact 721 ccatacatca cctcttactc agatacggct aaacccagtt tacaaagcag tagtgtcttc 781 tgacaaatct gggatgattg aatactggac tgggcctcct catgaatata aattccccaa 841 aaatgtgaac tgggaatata aaactgacac tgatttatat gaatttgcca agtgtaaggc 901 ttatccaacc agcgtatgtt tttcaccaga tgggaagaaa atagctacta ttggttctga 961 tagaaaagtt agaattttca gatttgtaac tggaaaactc atgagagtct ttgatgaatc 1021 actaagcatg tttactgaac tgcaacagat gaggcaacag ttaccagaca tggaatttgg 1081 ccgacgaatg gctgtagaac gtgagttgga gaaggttgat gctgtaagat taattaatat 1141 agtttttgat gaaactggac acttcgtgct gtatggaaca atgctgggca ttaaagttat 1201 aaatgtagag acaaaccggt gtgtgcggat tttaggcaaa caagaaaata ttagagtgat 1261 gcaattggct ttgttccagg ggatagccaa aaagcatcgt gctgcaacta ctatagaaat 1321 gaaagcttct gaaaatcctg ttcttcagaa tattcaagct gacccaacaa tagtctgtac 1381 atcattcaaa aagaatagat tttatatgtt taccaaacga gaaccagaag atacgaaaag 1441 tgcagattct gatcgagatg tttttaatga gaaaccttct aaagaagaag tcatggcagc 1501 tactcaagct gaaggaccta aacgagtttc ggacagtgcc attatccaca ccagcatggg 1561 agacattcac accaaacttt ttcctgttga gtgccctaag acagtggaaa acttctgtgt 1621 tcacagcaga aatggttatt ataatgggca tacatttcac cgtataatta agggctttat 1681 gattcagact GGAGATCCAA CAGGTACTGG TATGGGAGGA GAAAGCATAT GGGGAGGAGA 1741 ATTTGAAGAT GAATTTCATT CAACATTACG ACATGACAGG CCGTACACAC TCAGCATGGC 1801 TAACGCGGGA TCAAATACTA ATGGATCCCA GTTTTTCATA ACGGTAGTAC CAACGCCTTG 1861 GCTTGATAAT AAGCATACAG TATTTGGACG AGTGACTAAA GGAATGGAAG TTGTACAGAG 1921 GATCTCCAAC GTCAAAGTCA ATCCCAAAAC AGATAAGCCC TATGAGGATG TCAGCATCAT 1981 AAATATTACT GTCAAGTAAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 2041 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 2101 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTCGG CTCCACCTAC 2161 GGTCTATAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt