Construct: ORF TRCN0000488933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021172.1_s317c1
- DNA Barcode:
- AGTTCATCGCAAGCACCCGTTTCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RHO (6010)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488933
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6010 | RHO | rhodopsin | NM_000539.3 | 100% | 100% | |
| 2 | mouse | 212541 | Rho | rhodopsin | NM_145383.1 | 88.3% | 94.8% | (many diffs) |
| 3 | mouse | 212541 | Rho | rhodopsin | XM_006505860.2 | 86.4% | 91% | (many diffs) |
| 4 | mouse | 212541 | Rho | rhodopsin | XM_006505861.2 | 85.9% | 90.8% | (many diffs) |
| 5 | mouse | 212541 | Rho | rhodopsin | XM_017321493.1 | 79.1% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1116
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaatggc acagaaggcc ctaacttcta cgtgcccttc tccaatgcga 121 cgggtgtggt acgcagcccc ttcgagtacc cacagtacta cctggctgag ccatggcagt 181 tctccatgct ggccgcctac atgtttctgc tgatcgtgct gggcttcccc atcaacttcc 241 tcacgctcta cgtcaccgtc cagcacaaga agctgcgcac gcctctcaac tacatcctgc 301 tcaacctagc cgtggctgac ctcttcatgg tcctaggtgg cttcaccagc accctctaca 361 cctctctgca tggatacttc gtcttcgggc ccacaggatg caatttggag ggcttctttg 421 ccaccctggg cggtgaaatt gccctgtggt ccttggtggt cctggccatc gagcggtacg 481 tggtggtgtg taagcccatg agcaacttcc gcttcgggga gaaccatgcc atcatgggcg 541 ttgccttcac ctgggtcatg gcgctggcct gcgccgcacc cccactcgcc ggctggtcca 601 ggtacatccc cgagggcctg cagtgctcgt gtggaatcga ctactacacg ctcaagccgg 661 aggtcaacaa cgagtctttt gtcatctaca tgttcgtggt ccacttcacc atccccatga 721 ttatcatctt tttctgctat gggcagctcG TCTTCACCGT CAAGGAGGCC GCTGCCCAGC 781 AGCAGGAGTC AGCCACCACA CAGAAGGCAG AGAAGGAGGT CACCCGCATG GTCATCATCA 841 TGGTCATCGC TTTCCTGATC TGCTGGGTGC CCTACGCCAG CGTGGCATTC TACATCTTCA 901 CCCACCAGGG CTCCAACTTC GGTCCCATCT TCATGACCAT CCCAGCGTTC TTTGCCAAGA 961 GCGCCGCCAT CTACAACCCT GTCATCTATA TCATGATGAA CAAGCAGTTC CGGAACTGCA 1021 TGCTCACCAC CATCTGCTGC GGCAAGAACC CACTGGGTGA CGATGAGGCC TCTGCTACCG 1081 TGTCCAAGAC GGAGACGAGC CAGGTGGCCC CGGCCTAAGA CCCAGCTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGAAGTTCA TCGCAAGCAC CCGTTTCTAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt