Construct: ORF TRCN0000488944
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020181.1_s317c1
- DNA Barcode:
- CCACTAAATTGGAGTTATGCTGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAPKAPK3 (7867)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488944
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7867 | MAPKAPK3 | MAPK activated protein kina... | NM_001243925.2 | 100% | 100% | |
| 2 | human | 7867 | MAPKAPK3 | MAPK activated protein kina... | NM_001243926.1 | 100% | 100% | |
| 3 | human | 7867 | MAPKAPK3 | MAPK activated protein kina... | NM_004635.4 | 100% | 100% | |
| 4 | human | 7867 | MAPKAPK3 | MAPK activated protein kina... | XR_940501.2 | 40.5% | 1_126del;629_630ins124;1149_2396del | |
| 5 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | NM_178907.3 | 89.6% | 94.2% | (many diffs) |
| 6 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | XM_006511616.3 | 89.6% | 94.2% | (many diffs) |
| 7 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | XM_006511617.3 | 76.4% | 78.6% | (many diffs) |
| 8 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | XM_006511618.3 | 75% | 79.1% | (many diffs) |
| 9 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | XM_006511619.1 | 65.7% | 70.1% | (many diffs) |
| 10 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | NM_001316691.1 | 62.1% | 58% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1218
- ORF length:
- 1146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatggt gaaacagcag aggagcaggg gggccctgtg cccccgccag 121 ttgcacccgg cggacccggc ttgggcggtg ctccgggggg gcggcgggag cccaagaagt 181 acgcagtgac cgacgactac cagttgtcca agcaggtgct gggcctgggt gtgaacggca 241 aagtgctgga gtgcttccat cggcgcactg gacagaagtg tgccctgaag ctcctgtatg 301 acagccccaa ggcccggcag gaggtagacc atcactggca ggcttctggc ggcccccata 361 ttgtctgcat cctggatgtg tatgagaaca tgcaccatgg caagcgctgt ctcctcatca 421 tcatggaatg catggaaggt ggtgagttgt tcagcaggat tcaggagcgt ggcgaccagg 481 ctttcactga gagagaagct gcagagataa tgcgggatat tggcactgcc atccagtttc 541 tgcacagcca taacattgcc caccgagatg tcaagcctga aaacctactc tacacatcta 601 aggagaaaga cgcagtgctt aagctcaccg attttggctt tgctaaggag accacccaaa 661 atgccctgca gacaccctgc tatactccct attatgtggc ccctgaggtc ctgggtccag 721 agaagtatga caagtcatgt gacatgtggt ccctgggtgt catcatgtac atcctccttt 781 gtggcttccc acccttctac tccaacacgg gccaggccat ctccccgggg atgaagagga 841 ggattcgcct gggccagtac ggcttcccca atcctgagtg gtcagaagtc tctgaggatg 901 ccaagcagct gatccgcctc ctgttGAAGA CAGACCCCAC AGAGAGGCTG ACCATCACTC 961 AGTTCATGAA CCACCCCTGG ATCAACCAAT CGATGGTAGT GCCACAGACC CCACTCCACA 1021 CGGCCCGAGT GCTGCAGGAG GACAAAGACC ACTGGGACGA AGTCAAGGAG GAGATGACCA 1081 GTGCCTTGGC CACTATGCGG GTAGACTACG ACCAGGTGAA GATCAAGGAC CTGAAGACCT 1141 CTAACAACCG GCTCCTCAAC AAGAGGAGAA AAAAGCAGGC AGGCAGCTCC TCTGCCTCAC 1201 AGGGCTGCAA CAACCAGTGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCAC TAAATTGGAG 1381 TTATGCTGCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt