Construct: ORF TRCN0000488948

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020083.1_s317c1
DNA Barcode:
GCCAACGTGAGACAAAAACCATAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EPHB2 (2048)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488948
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2048 EPHB2 EPH receptor B2 NM_017449.4 99.9% 99.8% 2958_2959insG
2 human 2048 EPHB2 EPH receptor B2 NM_004442.7 99.8% 99.7% 1699_1701delAGA;2961_2962insG
3 human 2048 EPHB2 EPH receptor B2 XM_006710441.4 98.7% 97.1% (many diffs)
4 human 2048 EPHB2 EPH receptor B2 XM_006710442.4 96.6% 96.6% (many diffs)
5 human 2048 EPHB2 EPH receptor B2 NM_001309192.2 94% 93.9% 1545T>C;1591_1592ins174;2784_2785insG
6 human 2048 EPHB2 EPH receptor B2 NM_001309193.2 93.3% 93.3% 1545T>C;2956_2957insTT;2958_3165del
7 human 2048 EPHB2 EPH receptor B2 XM_024453895.1 55.2% 55.2% 0_1ins1323;222T>C;1635_1636insG
8 mouse 13844 Ephb2 Eph receptor B2 NM_010142.4 90.9% 99.3% (many diffs)
9 mouse 13844 Ephb2 Eph receptor B2 NM_001290753.2 90.8% 99.2% (many diffs)
10 mouse 13844 Ephb2 Eph receptor B2 XM_006538531.3 90.8% 99.2% (many diffs)
11 mouse 13844 Ephb2 Eph receptor B2 XM_006538534.2 90.7% 99.1% (many diffs)
12 mouse 13844 Ephb2 Eph receptor B2 XM_006538533.3 87.7% 96.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3030
ORF length:
2961
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggctctgcgg aggctggggg ccgcgctgct gctgctgccg ctgctcgccg
121ccgtggaaga aacgctaatg gactccacta cagcgactgc tgagctgggc tggatggtgc
181atcctccatc agggtgggaa gaggtgagtg gctacgatga gaacatgaac acgatccgca
241cgtaccaggt gtgcaacgtg tttgagtcaa gccagaacaa ctggctacgg accaagttta
301tccggcgccg tggcgcccac cgcatccacg tggagatgaa gttttcggtg cgtgactgca
361gcagcatccc cagcgtgcct ggctcctgca aggagacctt caacctctat tactatgagg
421ctgactttga ctcggccacc aagaccttcc ccaactggat ggagaatcca tgggtgaagg
481tggataccat tgcagccgac gagagcttct cccaggtgga cctgggtggc cgcgtcatga
541aaatcaacac cgaggtgcgg agcttcggac ctgtgtcccg cagcggcttc tacctggcct
601tccaggacta tggcggctgc atgtccctca tcgccgtgcg tgtcttctac cgcaagtgcc
661cccgcatcat ccagaatggc gccatcttcc aggaaaccct gtcgggggct gagagcacat
721cgctggtggc tgcccggggc agctgcatcg ccaatgcgga agaggtggat gtacccatca
781agctctactg taacggggac ggcgagtggc tggtgcccat cgggcgctgc atgtgcaaag
841caggcttcga ggccgttgag aatggcaccg tctgccgagg ttgtccatct gggactttca
901aggccaacca aggggatgag gcctgtaccc actgtcccat caacagccgg accacttctg
961aaggggccac caactgtgtc tgccgcaatg gctactacag agcagacctg gaccccctgg
1021acatgccctg cacaaccatc ccctccgcgc cccaggctgt gatttccagt gtcaatgaga
1081cctccctcat gctggagtgg acccctcccc gcgactccgg aggccgagag gacctcgtct
1141acaacatcat ctgcaagagc tgtggctcgg gccggggtgc ctgcacccgc tgcggggaca
1201atgtacagta cgcaccacgc cagctaggcc tgaccgagcc acgcatttac atcagtgacc
1261tgctggccca cacccagtac accttcgaga tccaggctgt gaacggcgtt actgaccaga
1321gccccttctc gcctcagttc gcctctgtga acatcaccac caaccaggca gctccatcgg
1381cagtgtccat catgcatcag gtgagccgca ccgtggacag cattaccctg tcgtggtccc
1441agccggacca gcccaatggc gtgatcctgg actatgagct gcagtactat gagaaggagc
1501tcagtgagta caacgccaca gccataaaaa gccccaccaa cacggtcacc gtgcagggcc
1561tcaaagccgg cgccatctat gtcttccagg tgcgggcacg caccgtggca ggctacgggc
1621gctacagcgg caagatgtac ttccagacca tgacagaagc cgagtaccag acaagcatcc
1681aggagaagtt gccactcatc atcggctcct cggccgctgg cctggtcttc ctcattgctg
1741tggttgtcat cgccatcgtg tgtaacagac gggggtttga gcgtgctgac tcggagtaca
1801cggacaagct gcaacactac accagtggcc acatgacccc aggcatgaag atctacatcg
1861atcctttcac ctacgaggac cccaacgagg cagtgcggga gtttgccaag gaaattgaca
1921tctcctgtgt caaaattgag caggtgatcg gagcagggga gtttggcgag gtctgcagtg
1981gccacctgaa gctgccaggc aagagagaga tctttgtggc catcaagacg ctcaagtcgg
2041gctacacgga gaagcagcgc cgggacttcc tgagcgaagc ctccatcatg ggccagttcg
2101accatcccaa cgtcatccac ctggagggtg tcgtgaccaa gagcacacct gtgatgatca
2161tcaccgagtt catggagaat ggctccctgg actcctttct ccggcaaaac gatgggcagt
2221tcacagtcat ccagctggtg ggcatgcttc ggggcatcgc agctggcatg aagtacctgg
2281cagacatgaa ctatgttcac cgtgacctgg ctgcccgcaa catcctcgtc aacagcaacc
2341tggtctgcaa ggtgtcggac tttgggctct cacgctttct agaggacgat acctcagacc
2401ccacctacac cagtgccctg ggcggaaaga tccccatccg ctggacagcc ccggaagcca
2461tccagtaccg gaagttcacc tcggccagtg atgtgtggag ctacggcatt gtcatgtggg
2521aggtgatgtc ctatggggag cggccctact gggacatgac caaccaggat gtaatcaatg
2581ccattgagca ggactatcgg ctgccaccgc ccatggactg cccgagcgcc ctgcaccaac
2641tcatgctgga ctgttgGCAG AAGGACCGCA ACCACCGGCC CAAGTTCGGC CAAATTGTCA
2701ACACGCTAGA CAAGATGATC CGCAATCCCA ACAGCCTCAA AGCCATGGCG CCCCTCTCCT
2761CTGGCATCAA CCTGCCGCTG CTGGACCGCA CGATCCCCGA CTACACCAGC TTTAACACGG
2821TGGACGAGTG GCTGGAGGCC ATCAAGATGG GGCAGTACAA GGAGAGCTTC GCCAATGCCG
2881GCTTCACCTC CTTTGACGTC GTGTCTCAGA TGATGATGGA GGACATTCTC CGGGTTGGGG
2941TCACTTTGGC TGGCCACCAG AAAAAAATCC TGAACAGTAT CCAGGTGATG CGGGCGCAGA
3001TGAACCAGAT TCAGTCTGTG GAGGTTGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
3061GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
3121AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCAACGT
3181GAGACAAAAA CCATAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
3241aagatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.