Construct: ORF TRCN0000488948
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020083.1_s317c1
- DNA Barcode:
- GCCAACGTGAGACAAAAACCATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHB2 (2048)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488948
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2048 | EPHB2 | EPH receptor B2 | NM_017449.4 | 99.9% | 99.8% | 2958_2959insG |
| 2 | human | 2048 | EPHB2 | EPH receptor B2 | NM_004442.7 | 99.8% | 99.7% | 1699_1701delAGA;2961_2962insG |
| 3 | human | 2048 | EPHB2 | EPH receptor B2 | XM_006710441.4 | 98.7% | 97.1% | (many diffs) |
| 4 | human | 2048 | EPHB2 | EPH receptor B2 | XM_006710442.4 | 96.6% | 96.6% | (many diffs) |
| 5 | human | 2048 | EPHB2 | EPH receptor B2 | NM_001309192.2 | 94% | 93.9% | 1545T>C;1591_1592ins174;2784_2785insG |
| 6 | human | 2048 | EPHB2 | EPH receptor B2 | NM_001309193.2 | 93.3% | 93.3% | 1545T>C;2956_2957insTT;2958_3165del |
| 7 | human | 2048 | EPHB2 | EPH receptor B2 | XM_024453895.1 | 55.2% | 55.2% | 0_1ins1323;222T>C;1635_1636insG |
| 8 | mouse | 13844 | Ephb2 | Eph receptor B2 | NM_010142.4 | 90.9% | 99.3% | (many diffs) |
| 9 | mouse | 13844 | Ephb2 | Eph receptor B2 | NM_001290753.2 | 90.8% | 99.2% | (many diffs) |
| 10 | mouse | 13844 | Ephb2 | Eph receptor B2 | XM_006538531.3 | 90.8% | 99.2% | (many diffs) |
| 11 | mouse | 13844 | Ephb2 | Eph receptor B2 | XM_006538534.2 | 90.7% | 99.1% | (many diffs) |
| 12 | mouse | 13844 | Ephb2 | Eph receptor B2 | XM_006538533.3 | 87.7% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3030
- ORF length:
- 2961
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggctctgcgg aggctggggg ccgcgctgct gctgctgccg ctgctcgccg 121 ccgtggaaga aacgctaatg gactccacta cagcgactgc tgagctgggc tggatggtgc 181 atcctccatc agggtgggaa gaggtgagtg gctacgatga gaacatgaac acgatccgca 241 cgtaccaggt gtgcaacgtg tttgagtcaa gccagaacaa ctggctacgg accaagttta 301 tccggcgccg tggcgcccac cgcatccacg tggagatgaa gttttcggtg cgtgactgca 361 gcagcatccc cagcgtgcct ggctcctgca aggagacctt caacctctat tactatgagg 421 ctgactttga ctcggccacc aagaccttcc ccaactggat ggagaatcca tgggtgaagg 481 tggataccat tgcagccgac gagagcttct cccaggtgga cctgggtggc cgcgtcatga 541 aaatcaacac cgaggtgcgg agcttcggac ctgtgtcccg cagcggcttc tacctggcct 601 tccaggacta tggcggctgc atgtccctca tcgccgtgcg tgtcttctac cgcaagtgcc 661 cccgcatcat ccagaatggc gccatcttcc aggaaaccct gtcgggggct gagagcacat 721 cgctggtggc tgcccggggc agctgcatcg ccaatgcgga agaggtggat gtacccatca 781 agctctactg taacggggac ggcgagtggc tggtgcccat cgggcgctgc atgtgcaaag 841 caggcttcga ggccgttgag aatggcaccg tctgccgagg ttgtccatct gggactttca 901 aggccaacca aggggatgag gcctgtaccc actgtcccat caacagccgg accacttctg 961 aaggggccac caactgtgtc tgccgcaatg gctactacag agcagacctg gaccccctgg 1021 acatgccctg cacaaccatc ccctccgcgc cccaggctgt gatttccagt gtcaatgaga 1081 cctccctcat gctggagtgg acccctcccc gcgactccgg aggccgagag gacctcgtct 1141 acaacatcat ctgcaagagc tgtggctcgg gccggggtgc ctgcacccgc tgcggggaca 1201 atgtacagta cgcaccacgc cagctaggcc tgaccgagcc acgcatttac atcagtgacc 1261 tgctggccca cacccagtac accttcgaga tccaggctgt gaacggcgtt actgaccaga 1321 gccccttctc gcctcagttc gcctctgtga acatcaccac caaccaggca gctccatcgg 1381 cagtgtccat catgcatcag gtgagccgca ccgtggacag cattaccctg tcgtggtccc 1441 agccggacca gcccaatggc gtgatcctgg actatgagct gcagtactat gagaaggagc 1501 tcagtgagta caacgccaca gccataaaaa gccccaccaa cacggtcacc gtgcagggcc 1561 tcaaagccgg cgccatctat gtcttccagg tgcgggcacg caccgtggca ggctacgggc 1621 gctacagcgg caagatgtac ttccagacca tgacagaagc cgagtaccag acaagcatcc 1681 aggagaagtt gccactcatc atcggctcct cggccgctgg cctggtcttc ctcattgctg 1741 tggttgtcat cgccatcgtg tgtaacagac gggggtttga gcgtgctgac tcggagtaca 1801 cggacaagct gcaacactac accagtggcc acatgacccc aggcatgaag atctacatcg 1861 atcctttcac ctacgaggac cccaacgagg cagtgcggga gtttgccaag gaaattgaca 1921 tctcctgtgt caaaattgag caggtgatcg gagcagggga gtttggcgag gtctgcagtg 1981 gccacctgaa gctgccaggc aagagagaga tctttgtggc catcaagacg ctcaagtcgg 2041 gctacacgga gaagcagcgc cgggacttcc tgagcgaagc ctccatcatg ggccagttcg 2101 accatcccaa cgtcatccac ctggagggtg tcgtgaccaa gagcacacct gtgatgatca 2161 tcaccgagtt catggagaat ggctccctgg actcctttct ccggcaaaac gatgggcagt 2221 tcacagtcat ccagctggtg ggcatgcttc ggggcatcgc agctggcatg aagtacctgg 2281 cagacatgaa ctatgttcac cgtgacctgg ctgcccgcaa catcctcgtc aacagcaacc 2341 tggtctgcaa ggtgtcggac tttgggctct cacgctttct agaggacgat acctcagacc 2401 ccacctacac cagtgccctg ggcggaaaga tccccatccg ctggacagcc ccggaagcca 2461 tccagtaccg gaagttcacc tcggccagtg atgtgtggag ctacggcatt gtcatgtggg 2521 aggtgatgtc ctatggggag cggccctact gggacatgac caaccaggat gtaatcaatg 2581 ccattgagca ggactatcgg ctgccaccgc ccatggactg cccgagcgcc ctgcaccaac 2641 tcatgctgga ctgttgGCAG AAGGACCGCA ACCACCGGCC CAAGTTCGGC CAAATTGTCA 2701 ACACGCTAGA CAAGATGATC CGCAATCCCA ACAGCCTCAA AGCCATGGCG CCCCTCTCCT 2761 CTGGCATCAA CCTGCCGCTG CTGGACCGCA CGATCCCCGA CTACACCAGC TTTAACACGG 2821 TGGACGAGTG GCTGGAGGCC ATCAAGATGG GGCAGTACAA GGAGAGCTTC GCCAATGCCG 2881 GCTTCACCTC CTTTGACGTC GTGTCTCAGA TGATGATGGA GGACATTCTC CGGGTTGGGG 2941 TCACTTTGGC TGGCCACCAG AAAAAAATCC TGAACAGTAT CCAGGTGATG CGGGCGCAGA 3001 TGAACCAGAT TCAGTCTGTG GAGGTTGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3061 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3121 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCAACGT 3181 GAGACAAAAA CCATAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3241 aagatt