Construct: ORF TRCN0000488949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019937.1_s317c1
- DNA Barcode:
- TGGCGGGTCCATAGGTCCTAGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V1C1 (528)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488949
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 528 | ATP6V1C1 | ATPase H+ transporting V1 s... | NM_001695.5 | 99.9% | 99.7% | 1146_1147insG |
| 2 | mouse | 66335 | Atp6v1c1 | ATPase, H+ transporting, ly... | NM_025494.3 | 89.3% | 97.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1221
- ORF length:
- 1149
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgactgag ttctggctta tatctgctcc tggggagaaa acctgtcagc 121 aaacatggga gaaattgcat gcggcaactt caaagaacaa taatcttgct gtcacttcca 181 agttcaatat tcctgactta aaggttggca cgttggatgt cttggttggc ttgtcagatg 241 aactggctaa actggatgca tttgtagaag gagtggttaa gaaagtagct caatacatgg 301 ctgatgtatt ggaagatagc aaagacaaag ttcaagagaa tctgttggct aatggagtgg 361 acttggttac ttatataaca aggttccagt gggacatggc caaatatcca atcaagcagt 421 ccctgaaaaa tatttctgaa ataattgcca agggagtaac tcagattgat aatgacctga 481 aatctcgagc atctgcatac aataacctga aaggaaatct tcagaatttg gaacgaaaga 541 atgcaggaag tttgctaact agaagtctag cagaaattgt gaagaaggat gactttgttc 601 ttgattcaga gtatctcgtc acattactgg tagtagttcc caagttaaac cacaacgact 661 ggattaagca gtatgaaaca ctagccgaaa tggtagttcc aaggtctagc aatgttcttt 721 cagaggacca agacagttac ctgtgtaatg tcaccttgtt taggaaggca gttgatgact 781 tcagacacaa agccagagaa aacaaattca ttgttcgtga cttccagtat aatgaagagg 841 agatgaaagc agataaagaa gaaatgaaca ggctttctac tgataagaaa aaacaatttG 901 GACCACTTGT ACGGTGGCTG AAAGTGAATT TTAGTGAAGC ATTTATTGCA TGGATTCACG 961 TGAAAGCATT ACGGGTTTTC GTTGAGTCTG TTTTAAGGTA TGGCTTGCCA GTGAACTTCC 1021 AAGCAATGCT ACTTCAGCCC AATAAGAAAA CTTTGAAGAA ACTGAGAGAA GTATTACATG 1081 AATTGTATAA ACATCTAGAC AGCAGTGCAG CAGCTATTAT TGATGCTCCT ATGGATATTC 1141 CAGGTTTAAA CCTGAGTCAA CAAGAATACT ACCCCTATGT GTACTACAAG ATTGATTGCA 1201 ACTTGCTGGA ATTCAAGGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGCGGG TCCATAGGTC 1381 CTAGTACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt