Construct: ORF TRCN0000488953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019531.1_s317c1
- DNA Barcode:
- AACGGCTTCCAACTAGCTATTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PYGB (5834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488953
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5834 | PYGB | glycogen phosphorylase B | NM_002862.4 | 99.9% | 99.8% | 1146T>C;2529_2530insG |
| 2 | human | 5834 | PYGB | glycogen phosphorylase B | XR_002958496.1 | 58.9% | (many diffs) | |
| 3 | mouse | 110078 | Pygb | brain glycogen phosphorylase | NM_153781.1 | 88.6% | 95.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2607
- ORF length:
- 2532
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcag 61 gcttccaatt gaccatggcg aagccgctga cggacagcga gaagcggaag cagatcagcg 121 tgcgcggcct ggcggggcta ggcgacgtgg ccgaggtgcg gaagagcttc aaccggcact 181 tgcacttcac gctggtcaag gaccgcaatg tggccacgcc ccgcgactac ttcttcgcgc 241 tggcgcacac ggtgcgcgac cacctcgtgg gccgctggat ccgcacgcag cagcactact 301 acgagcgcga ccccaagcgc atttattatc tttccctgga attctacatg ggtcgcacgc 361 tgcagaacac gatggtgaac ctgggccttc agaatgcctg cgatgaagcc atctatcagt 421 tggggttaga cttggaggaa ctcgaggaga tagaagaaga tgctggcctt gggaatggag 481 gcctggggag gctggcagcg tgtttccttg actcaatggc taccttgggc ctggcagcat 541 acggctatgg aatccgctat gaatttggga tttttaacca gaagattgtc aatggctggc 601 aggtagagga ggccgatgac tggctgcgct acggcaaccc ctgggagaaa gcgcggcctg 661 agtatatgct tcccgtgcac ttctacggac gcgtggagca cacccccgac ggcgtgaagt 721 ggctggacac acaggtggtg ctggccatgc cctacgacac cccagtgccc ggctacaaga 781 acaacaccgt caacaccatg cggctgtggt ccgccaaggc tcccaacgac ttcaagctgc 841 aggacttcaa cgtgggagac tacatcgagg cggtcctgga ccggaacttg gctgagaaca 901 tctccagggt cctgtatcca aatgataact tctttgaggg gaaggagctg cggctgaagc 961 aggagtactt cgtggtggcc gccacgctcc aggacatcat ccgccgcttc aagtcgtcca 1021 agttcggctg ccgggaccct gtgagaacct gtttcgagac gttcccagac aaggtggcca 1081 tccagctgaa cgacacccac cccgccctct ccatccctga gctcatgcgg atcctggtgg 1141 acgtggagaa ggtggactgg gacaaggcct gggaaatcac gaagaagacc tgtgcataca 1201 ccaaccacac tgtgctgccc gaggccttgg agcgctggcc cgtgtccatg tttgagaagc 1261 tgctgccgcg gcacctggag ataatctatg ccatcaacca gcggcacctg gaccacgtgg 1321 ccgcgctgtt tcccggcgat gtggaccgcc tgcgcaggat gtctgtgatc gaggaggggg 1381 actgcaagcg gatcaacatg gcccacctgt gtgtgattgg gtcccatgct gtcaatggtg 1441 tggcgaggat ccactcggag atcgtgaaac agtcggtctt taaggatttt tatgaactgg 1501 agccagagaa gttccagaat aagaccaatg gcatcacccc ccgccggtgg ctgctgctgt 1561 gcaacccggg gctggccgat accatcgtgg agaaaattgg ggaggagttc ctgactgacc 1621 tgagccagct gaagaagctg ctgccgctgg tcagtgacga ggtgttcatc agggacgtgg 1681 ccaaggtcaa acaggagaac aagctcaagt tctcggcctt cctggagaag gagtacaagg 1741 tgaagatcaa cccctcctcc atgttcgatg tgcatgtgaa gaggatccac gagtacaagc 1801 ggcagctgct caactgcctg cacgtcgtca ccctgtacaa tcgaatcaag agagacccgg 1861 ccaaggcttt tgtgcccagg actgttatga ttgggggcaa ggcagcgccc ggttaccaca 1921 tggccaagct gatcatcaag ttggtcacct ccatcggcga cgtcgtcaat catgacccag 1981 ttgtgggtga caggttgaaa gtgatcttcc tggagaacta ccgtgtgtcc ttggctgaga 2041 aagtgatccc ggccgctgat ctgtcgcagc agatctccac tgcaggcacc gaggcctcag 2101 gcacaggcaa catgaagttc atgctcaacg gggccctcac catcggcacc atggacggcg 2161 ccaacgtgga gatggccgag gaggccgggg ccgagaacct cttcatcttc ggcctgcggg 2221 tggaggatgt cgaggccttG GACCGGAAAG GGTACAATGC CAGGGAGTAC TACGACCACC 2281 TGCCCGAGCT GAAGCAGGCC GTGGACCAGA TCAGCAGTGG CTTTTTTTCT CCCAAGGAGC 2341 CAGACTGCTT CAAGGACATC GTGAACATGC TGATGCACCA TGACAGGTTC AAGGTGTTTG 2401 CAGACTATGA AGCCTACATG CAGTGCCAGG CACAGGTGGA CCAGCTGTAC CGGAACCCCA 2461 AGGAGTGGAC CAAGAAGGTC ATCAGGAACA TCGCCTGCTC GGGCAAGTTC TCCAGTGACC 2521 GGACCATCAC GGAGTATGCA CGGGAGATCT GGGGTGTGGA GCCCTCCGAC CTGCAGATCC 2581 CGCCCCCCAA CATCCCCCGG GACGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2641 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2701 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ACGGCTTCCA 2761 ACTAGCTATT CCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2821 att