Construct: ORF TRCN0000488960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021741.1_s317c1
- DNA Barcode:
- CCGCACTGGTTTAATACCTCTCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GAB2 (9846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488960
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9846 | GAB2 | GRB2 associated binding pro... | NM_080491.3 | 100% | 100% | |
| 2 | human | 9846 | GAB2 | GRB2 associated binding pro... | XM_024448782.1 | 96.8% | 96.7% | (many diffs) |
| 3 | human | 9846 | GAB2 | GRB2 associated binding pro... | NM_012296.3 | 94.3% | 94.3% | 0_1ins114 |
| 4 | human | 9846 | GAB2 | GRB2 associated binding pro... | XM_006718753.2 | 94.3% | 94.3% | 0_1ins114 |
| 5 | human | 9846 | GAB2 | GRB2 associated binding pro... | XM_011545408.3 | 67.4% | 67.4% | 0_1ins660 |
| 6 | mouse | 14389 | Gab2 | growth factor receptor boun... | NM_001162477.1 | 87.2% | 91.7% | (many diffs) |
| 7 | mouse | 14389 | Gab2 | growth factor receptor boun... | NM_010248.2 | 87% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2097
- ORF length:
- 2028
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gagcggcggc ggcgacgtgg tgtgcaccgg ctggctgagg aaatcgcctc 121 ccgagaagaa gttgaggcgc tatgcctgga agaaacgctg gtttatcctg cggagtggcc 181 ggatgagcgg tgacccagat gttctggaat actacaagaa cgatcactcc aagaagcctc 241 tgcggatcat caacctgaac ttctgtgagc aggtagatgc aggcctgacc tttaacaaga 301 aggagctgca ggatagtttt gtgtttgaca tcaagaccag tgaacgcacc ttttacctgg 361 tggctgagac agaagaggac atgaataagt gggtccagag catctgccag atctgtggct 421 tcaatcaggc tgaggagagc acagactccc tgagaaatgt ttcctcagcc ggtcatggcc 481 cccgctcttc tccagctgag ctcagcagct ctagccagca ccttctccga gagcgcaagt 541 cctcagcccc atcacactcc agccagccaa ctctgttcac gtttgaaccc cctgtgtcaa 601 accacatgca gcccaccttg tccaccagcg cacctcagga gtatctctac ttgcaccagt 661 gcataagccg aagagcagaa aatgcaagga gtgccagctt ctctcagggc accagagcct 721 cttttctcat gaggagtgac acagctgtac aaaaacttgc ccagggcaat ggacactgtg 781 tcaacgggat cagtggtcaa gtccatggct tctatagcct tcccaagccg agccggcaca 841 atacagaatt cagagacagt acctacgacc tcccccgcag cctggcctcc catggccaca 901 ccaagggcag cctcacaggc tccgagacag ataatgagga tgtgtacacc ttcaagacgc 961 ccagcaacac cctgtgcagg gagttcgggg acctcctggt agacaatatg gatgttccgg 1021 ccaccccact ctcagcctac cagatcccta ggacattcac tctggacaaa aaccacaatg 1081 ccatgacagt ggccactcct ggggactcag ccatagctcc cccaccccgc ccccccaagc 1141 caagtcaggc agaaacacct cgatggggca gtcctcagca gagaccgcca atcagtgaaa 1201 atagcagatc tgtcgctgcc accatcccca gacgcaacac cctccctgca atggacaaca 1261 gccgacttca ccgagcttct tcctgtgaga cctacgagta cccacagcgt ggtggagaga 1321 gtgcaggccg gtctgctgaa tccatgagtg atggagttgg ctctttcctg ccagggaaaa 1381 tgattgtggg ccgatcggac agcaccaatt ctgaagacaa ctatgtgccc atgaatccag 1441 gttcttccac cctgttggcc atggaacgag caggtgataa ttcccagagc gtctacatcc 1501 caatgagccc aggggcccat cactttgact cacttggcta cccatcaaca acccttcctg 1561 tgcaccgagg ccccagcaga ggaagtgaga ttcagccacc ccctgtcaac cgcaacctca 1621 aacctgatcg gaaagcaaag ccaacaccac ttgacctgag gaacaacacc gtcatcgatg 1681 aactcccctt caagtcacct atcaccaagt cttggtctag ggccaaccac accTTCAACT 1741 CCAGCTCCTC CCAGTACTGC CGCCCCATCT CCACCCAGAG CATCACCAGC ACAGACTCAG 1801 GAGACAGCGA AGAGAACTAT GTCCCTATGC AAAACCCAGT GTCTGCATCT CCCGTTCCCA 1861 GTGGCACGAA CAGTCCTGCC CCTAAGAAGA GCACCGGCAG CGTTGATTAT CTGGCCCTGG 1921 ACTTCCAGCC GAGCTCCCCA AGCCCCCACC GCAAGCCATC TACTTCATCC GTCACCTCTG 1981 ATGAGAAGGT GGACTACGTT CAGGTGGACA AGGAGAAGAC CCAGGCCCTG CAGAACACCA 2041 TGCAGGAGTG GACAGACGTG CGGCAGTCCT CAGAGCCTTC CAAGGGTGCC AAGCTGTAGA 2101 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2161 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2221 TTTATATATC TTGTGGAAAG GACGACCGCA CTGGTTTAAT ACCTCTCGTA CGCGTTAAGT 2281 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt