Construct: ORF TRCN0000488971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020565.1_s317c1
- DNA Barcode:
- AATTTTGGCCGCCAAAAATCGGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ITPKA (3706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488971
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3706 | ITPKA | inositol-trisphosphate 3-ki... | NM_002220.3 | 100% | 100% | |
| 2 | human | 3706 | ITPKA | inositol-trisphosphate 3-ki... | XM_011521522.2 | 62.6% | 62.6% | 0_1ins516 |
| 3 | mouse | 228550 | Itpka | inositol 1,4,5-trisphosphat... | NM_146125.2 | 87.6% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1455
- ORF length:
- 1383
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaccctg cccgggggcc caacgggcat ggcgcggccg gggggcgcga 121 ggccctgcag cccggggctg gagcgggccc cgcgccggag tgtcggggag ctgcgcctgc 181 tcttcgaggc gcgctgtgcg gcggtcgctg cggccgccgc cgcgggggag ccccgggccc 241 gcggggccaa gcggcgtggg ggacaggtcc ccaacgggct tccgcgggct cccccggccc 301 cggtgatccc tcagctgacc gtgacagccg aggagcccga cgtgcccccg accagccctg 361 ggccgccgga gcgggagagg gactgcctcc cggcagcggg ctcttcgcac ctgcagcagc 421 cgcgccgcct ttccacctcg tcggtctcct ccactggctc ctcgtcgctg ctcgaggact 481 cggaggacga cctgctgagc gacagtgaga gccggagccg cggcaacgtg cagctggaag 541 cgggcgagga cgtgggtcag aaaaaccact ggcagaagat ccggaccatg gtcaatctgc 601 cggtcataag ccctttcaag aagcgctacg cctgggtgca gctggcaggg cacactggga 661 gttttaaggc ggcgggcacc agcgggctga tcctgaagcg ctgctcggag ccggagcgct 721 actgcctggc gcggctgatg gctgacgcgc tgcgcggctg cgtgcctgcc ttccacggcg 781 tggtggagcg cgacggcgaa agctacctgc agctgcagga cctgctcgat ggcttcgacg 841 gaccttgtgt gctcgactgc aaaatgggcg tcaggactta cctagaggag gagctgacca 901 aggcccgtga gcggcccaag ctgcggaagg acatgtacaa gaaaatgctg gcggtggatc 961 ctgaagctcc cacggaggag gagcacgcgc agcgcgccgt caccaagccg cgctacatgc 1021 agtggcggga aggcatcagc tccagcacca ccctcggctt ccgcatcgag ggcatcaaga 1081 aagcggacgg ctcctgcagc accGACTTCA AGACTACGCG AAGCCGAGAG CAGGTGCTTC 1141 GCGTCTTTGA AGAGTTTGTG CAAGGAGATG AGGAAGTGCT GAGGCGGTAT CTGAACCGCC 1201 TGCAGCAGAT CCGGGACACC CTGGAGGTAT CCGAGTTCTT CAGGAGGCAC GAGGTGATCG 1261 GCAGCTCGCT CCTCTTTGTG CACGATCACT GCCATCGCGC CGGCGTGTGG CTCATCGACT 1321 TCGGCAAGAC CACGCCCCTC CCCGATGGCC AGATCCTGGA CCACCGGCGG CCCTGGGAGG 1381 AGGGCAACCG CGAGGACGGC TATTTGCTGG GGCTGGACAA TCTCATTGGC ATCCTGGCCA 1441 GCCTGGCTGA GAGATGAAAG CTTGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1501 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1561 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ATTTTGGCCG 1621 CCAAAAATCG GTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1681 att