Construct: ORF TRCN0000488972
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020390.1_s317c1
- DNA Barcode:
- CAACTGTTTAGCCTTTCCCAGCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKAA1 (5562)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488972
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_006251.5 | 98.3% | 98.3% | 1_27del;90T>C |
| 2 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355028.2 | 95.7% | 93.3% | (many diffs) |
| 3 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | XM_017009625.2 | 95.7% | 93.3% | (many diffs) |
| 4 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_206907.3 | 95.7% | 95.8% | 1_27del;90T>C;363_407del |
| 5 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | XM_017009624.1 | 93.2% | 90.8% | (many diffs) |
| 6 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355029.2 | 75.4% | 75.4% | 0_1ins405 |
| 7 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355035.2 | 54.7% | 54.7% | 0_1ins747 |
| 8 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355036.2 | 54.7% | 54.7% | 0_1ins747 |
| 9 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355037.2 | 54.7% | 54.7% | 0_1ins747 |
| 10 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355034.1 | 35.8% | 33.3% | (many diffs) |
| 11 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | NM_001013367.3 | 88.6% | 97.1% | (many diffs) |
| 12 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | XM_011245321.2 | 74.9% | 82% | (many diffs) |
| 13 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | XM_017316356.1 | 74.9% | 82% | (many diffs) |
| 14 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | XM_006519953.2 | 67.6% | 74.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1722
- ORF length:
- 1650
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgaca gccgagaagc agaaacacga cgggcgggtg aagatcggcc 121 actacattct gggcgacacg ctgggggtcg gcaccttcgg caaagtgaag gttggcaaac 181 atgaattgac tgggcataaa gtagctgtga agatactcaa tcgacagaag attcggagcc 241 ttgatgtggt aggaaaaatc cgcagagaaa ttcagaacct caagcttttc aggcatcctc 301 atataattaa actgtaccag gtcatcagta caccatctga tattttcatg gtgatggaat 361 atgtctcagg aggagagcta tttgattata tctgtaagaa tggaaggctg gatgaaaaag 421 aaagtcggcg tctgttccaa cagatccttt ctggtgtgga ttattgtcac aggcatatgg 481 tggtccatag agatttgaaa cctgaaaatg tcctgcttga tgcacacatg aatgcaaaga 541 tagctgattt tggtctttca aacatgatgt cagatggtga atttttaaga acaagttgtg 601 gctcacccaa ctatgctgca ccagaagtaa tttcaggaag attgtatgca ggcccagagg 661 tagatatatg gagcagtggg gttattctct atgctttatt atgtggaacc cttccatttg 721 atgatgacca tgtgccaact ctttttaaga agatatgtga tgggatcttc tatacccctc 781 aatatttaaa tccttctgtg attagccttt tgaaacatat gctgcaggtg gatcccatga 841 agagggccac aatcaaagat atcagggaac atgaatggtt taaacaggac cttccaaaat 901 atctctttcc tgaggatcca tcatatagtt caaccatgat tgatgatgaa gccttaaaag 961 aagtatgtga aaagtttgag tgctcagaag aggaagttct cagctgtctt tacaacagaa 1021 atcaccagga tcctttggca gttgcctacc atctcataat agataacagg agaataatga 1081 atgaagccaa agatttctat ttggcgacaa gcccacctga ttcttttctt gatgatcatc 1141 acctgactcg gccccatcct gaaagagtac cattcttggt tgctgaaaca ccaagggcac 1201 gccataccct tgatgaatta aatccacaga aatccaaaca ccaaggtgta aggaaagcaa 1261 aatggcattt aggaattaga agtcaaagtc gaccaaatga tattatggca gaagtatgta 1321 gagcaatcaa acaattggat tatgaatgga aggttGTAAA CCCATATTAT TTGCGTGTAC 1381 GAAGGAAGAA TCCTGTGACA AGCACTTACT CCAAAATGAG TCTACAGTTA TACCAAGTGG 1441 ATAGTAGAAC TTATCTACTG GATTTCCGTA GTATTGATGA TGAAATTACA GAAGCCAAAT 1501 CAGGGACTGC TACTCCACAG AGATCGGGAT CAGTTAGCAA CTATCGATCT TGCCAAAGGA 1561 GTGATTCAGA TGCTGAGGCT CAAGGAAAAT CCTCAGAAGT TTCTCTTACC TCATCTGTGA 1621 CCTCACTTGA CTCTTCTCCT GTTGACCTAA CTCCAAGACC TGGAAGTCAC ACAATAGAAT 1681 TTTTTGAGAT GTGTGCAAAT CTAATTAAAA TTCTTGCACA ATAGGACCCA GCTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA CAACTGTTTA GCCTTTCCCA GCGTACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt