Construct: ORF TRCN0000488985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019720.1_s317c1
- DNA Barcode:
- GTATGCCGGGCTCAGTCACGTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKCI (5584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5584 | PRKCI | protein kinase C iota | NM_002740.6 | 98.4% | 98.3% | 1_27del;1788_1789insG |
| 2 | human | 5584 | PRKCI | protein kinase C iota | XM_017006860.1 | 79% | 75.7% | 0_1ins283;54_55ins86;1392_1393insG |
| 3 | human | 5584 | PRKCI | protein kinase C iota | XR_001740206.1 | 34.7% | 1_192del;1367_1368ins88;1866_4722delinsG | |
| 4 | mouse | 18759 | Prkci | protein kinase C, iota | NM_008857.3 | 88.9% | 96.6% | (many diffs) |
| 5 | mouse | 18759 | Prkci | protein kinase C, iota | XM_006535411.3 | 84.5% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1839
- ORF length:
- 1764
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtcc cacacggtcg caggcggcgg cagcggggac cattcccacc 121 aggtccgggt gaaagcctac taccgcgggg atatcatgat aacacatttt gaaccttcca 181 tctcctttga gggcctttgc aatgaggttc gagacatgtg ttcttttgac aacgaacagc 241 tcttcaccat gaaatggata gatgaggaag gagacccgtg tacagtatca tctcagttgg 301 agttagaaga agcctttaga ctttatgagc taaacaagga ttctgaactc ttgattcatg 361 tgttcccttg tgtaccagaa cgtcctggga tgccttgtcc aggagaagat aaatccatct 421 accgtagagg tgcacgccgc tggagaaagc tttattgtgc caatggccac actttccaag 481 ccaagcgttt caacaggcgt gctcactgtg ccatctgcac agaccgaata tggggacttg 541 gacgccaagg atataagtgc atcaactgca aactcttggt tcataagaag tgccataaac 601 tcgtcacaat tgaatgtggg cggcattctt tgccacagga accagtgatg cccatggatc 661 agtcatccat gcattctgac catgcacaga cagtaattcc atataatcct tcaagtcatg 721 agagtttgga tcaagttggt gaagaaaaag aggcaatgaa caccagggaa agtggcaaag 781 cttcatccag tctaggtctt caggattttg atttgctccg ggtaatagga agaggaagtt 841 atgccaaagt actgttggtt cgattaaaaa aaacagatcg tatttatgca atgaaagttg 901 tgaaaaaaga gcttgttaat gatgatgagg atattgattg ggtacagaca gagaagcatg 961 tgtttgagca ggcatccaat catcctttcc ttgttgggct gcattcttgc tttcagacag 1021 aaagcagatt gttctttgtt atagagtatg taaatggagg agacctaatg tttcatatgc 1081 agcgacaaag aaaacttcct gaagaacatg ccagatttta ctctgcagaa atcagtctag 1141 cattaaatta tcttcatgag cgagggataa tttatagaga tttgaaactg gacaatgtat 1201 tactggactc tgaaggccac attaaactca ctgactacgg catgtgtaag gaaggattac 1261 ggccaggaga tacaaccagc actttctgtg gtactcctaa ttacattgct cctgaaattt 1321 taagaggaga agattatggt ttcagtgttg actggtgggc tcttggagtg ctcatgtttg 1381 agatgatggc aggaaggtct ccatttgata ttgttgggag ctccgataac cctgaccaga 1441 acacagagga ttatctcttc caagttattt tGGAAAAACA AATTCGCATA CCACGTTCTC 1501 TGTCTGTAAA AGCTGCAAGT GTTCTGAAGA GTTTTCTTAA TAAGGACCCT AAGGAACGAT 1561 TGGGTTGTCA TCCTCAAACA GGATTTGCTG ATATTCAGGG ACACCCGTTC TTCCGAAATG 1621 TTGATTGGGA TATGATGGAG CAAAAACAGG TGGTACCTCC CTTTAAACCA AATATTTCTG 1681 GGGAATTTGG TTTGGACAAC TTTGATTCTC AGTTTACTAA TGAACCTGTC CAGCTCACTC 1741 CAGATGACGA TGACATTGTG AGGAAGATTG ATCAGTCTGA ATTTGAAGGT TTTGAGTATA 1801 TCAATCCTCT TTTGATGTCT GCAGAAGAAT GTGTCGACCC AGCTTTCTTG TACAAAGTGG 1861 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1921 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1981 AGTATGCCGG GCTCAGTCAC GTCTAACGCG TTAAGTCgac aatcaacctc tggattacaa 2041 aatttgtgaa agatt