Construct: ORF TRCN0000488989
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021936.1_s317c1
- DNA Barcode:
- TCGTCCAGGGCTAAACAGCTGTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- WWTR1 (25937)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488989
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25937 | WWTR1 | WW domain containing transc... | NM_001168278.2 | 100% | 100% | |
| 2 | human | 25937 | WWTR1 | WW domain containing transc... | NM_001168280.3 | 100% | 100% | |
| 3 | human | 25937 | WWTR1 | WW domain containing transc... | NM_001348362.1 | 100% | 100% | |
| 4 | human | 25937 | WWTR1 | WW domain containing transc... | NM_015472.6 | 100% | 100% | |
| 5 | human | 25937 | WWTR1 | WW domain containing transc... | XM_017006122.1 | 100% | 100% | |
| 6 | mouse | 97064 | Wwtr1 | WW domain containing transc... | NM_133784.3 | 88.1% | 91.2% | (many diffs) |
| 7 | mouse | 97064 | Wwtr1 | WW domain containing transc... | NM_001168281.1 | 77.2% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1269
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaatccggcc tcggcgcccc ctccgctccc gccgcctggg cagcaagtga 121 tccacgtcac gcaggaccta gacacagacc tcgaagccct cttcaactct gtcatgaatc 181 cgaagcctag ctcgtggcgg aagaagatcc tgccggagtc tttctttaag gagcctgatt 241 cgggctcgca ctcgcgccag tccagcaccg actcgtcggg cggccacccg gggcctcgac 301 tggctggggg tgcccagcat gtccgctcgc actcgtcgcc cgcgtccctg cagctgggca 361 ccggcgcggg tgctgcgggt agccccgcgc agcagcacgc gcacctccgc cagcagtcct 421 acgacgtgac cgacgagctg ccactgcccc cgggctggga gatgaccttc acggccactg 481 gccagaggta cttcctcaat cacatagaaa aaatcaccac atggcaagac cctaggaagg 541 cgatgaatca gcctctgaat catatgaacc tccaccctgc cgtcagttcc acaccagtgc 601 ctcagaggtc catggcagta tcccagccaa atctcgtgat gaatcaccaa caccagcagc 661 agatggcccc cagtaccctg agccagcaga accaccccac tcagaaccca cccgcagggc 721 tcatgagtat gcccaatgcg ctgaccactc agcagcagca gcagcagaaa ctgcggcttc 781 agagaatcca gatggagaga gaaaggattc gaatgcgcca agaggagctc atgaggcagg 841 aagctgccct ctgtcgacag ctccccatgg aagctgagac tcttgcccca gttcaggctg 901 ctgtcaaccc acccacgatg accccagaca tgagatccat cactaataat agctcagatc 961 ctttcctcaa tGGAGGGCCA TATCATTCGA GGGAGCAGAG CACTGACAGT GGCCTGGGGT 1021 TAGGGTGCTA CAGTGTCCCC ACAACTCCGG AGGACTTCCT CAGCAATGTG GATGAGATGG 1081 ATACAGGAGA AAACGCAGGA CAAACACCCA TGAACATCAA TCCCCAACAG ACCCGTTTCC 1141 CTGATTTCCT TGACTGTCTT CCAGGAACAA ACGTTGACTT AGGAACTTTG GAATCTGAAG 1201 ACCTGATCCC CCTCTTCAAT GATGTAGAGT CTGCTCTGAA CAAAAGTGAG CCCTTTCTAA 1261 CCTGGCTGTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCG TCCAGGGCTA AACAGCTGTC 1441 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t