Construct: ORF TRCN0000488992
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021728.1_s317c1
- DNA Barcode:
- TTACAATGCTTAACTTCGTTATCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EPHA4 (2043)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488992
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2043 | EPHA4 | EPH receptor A4 | NM_001304537.2 | 43% | 1_1597del | |
| 2 | human | 2043 | EPHA4 | EPH receptor A4 | NM_001304536.1 | 40.8% | 1_1750del | |
| 3 | human | 2043 | EPHA4 | EPH receptor A4 | NM_004438.5 | 40.8% | 1_1750del | |
| 4 | human | 2043 | EPHA4 | EPH receptor A4 | NM_001363748.2 | 37% | 1_1750del;2847_2848ins111 | |
| 5 | mouse | 13838 | Epha4 | Eph receptor A4 | XM_006496408.1 | 38.9% | (many diffs) | |
| 6 | mouse | 13838 | Epha4 | Eph receptor A4 | NM_007936.3 | 36.9% | (many diffs) | |
| 7 | mouse | 13838 | Epha4 | Eph receptor A4 | XM_006496407.2 | 33.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 73
- ORF end:
- 88
- ORF length:
- 15
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctcagc ggatgaagag aaacatttga atcaaggtgt aagaacatat gtggacccct 121 ttacgtacga agatcccaac caagcagtgc gagagtttgc caaagaaatt gacgcatcct 181 gcattaagat tgaaaaagtt ataggagttg gtgaatttgg tgaggtatgc agtgggcgtc 241 tcaaagtgcc tggcaagaga gagatctgtg tggctatcaa gactctgaaa gctggttata 301 cagacaaaca gaggagagac ttcctgagtg aggccagcat catgggacag tttgaccatc 361 cgaacatcat tcacttggaa ggcgtggtca ctaaatgtaa accagtaatg atcataacag 421 agtacatgga gaatggctcc ttggatgcat tcctcaggaa aaatgatggc agatttacag 481 tcattcagct ggtgggcatg cttcgtggca ttgggtctgg gatgaagtat ttatctgata 541 tgagctatgt gcatcgtgat ctggccgcac ggaacatcct ggtgaacagc aacttggtct 601 gcaaagtgtc tgattttggc atgtcccgag tgcttgagga tgatccggaa gcagcttaca 661 ccaccagggg tggcaagatt cctatccggt ggactgcgcc agaagcaatt gcctatcgta 721 aattcacatc agcaagtgat gtatggagct atggaatcgt tatgtgggaa gtgatgtcgt 781 acggggagag gccctattgg gatatgtcca atcaagatgt gattaaagcc attgaggaag 841 gctatcggtt accccctcca atggactgcc ccattgcgct ccaccagctg atgctagact 901 gctggcagaa ggagaggagc gacaggccta aatttgggca gattgtcaac atgttGGACA 961 AACTCATCCG CAACCCCAAC AGCTTGAAGA GGACAGGGAC GGAGAGCTCC AGACCTAACA 1021 CTGCCTTGTT GGATCCAAGC TCCCCTGAAT TCTCTGCTGT GGTATCAGTG GGCGATTGGC 1081 TCCAGGCCAT TAAAATGGAC CGGTATAAGG ATAACTTCAC AGCTGCTGGT TATACCACAC 1141 TAGAGGCTGT GGTGCACGTG AACCAGGAGG ACCTGGCAAG AATTGGTATC ACAGCCATCA 1201 CGCACCAGAA TAAGATTTTG AGCAGTGTCC AGGCAATGCG AACCCAAATG CAGCAGATGC 1261 ACGGCAGAAT GGTTCCCGTC TGAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TACAATGCTT 1441 AACTTCGTTA TCTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1501 att