Construct: ORF TRCN0000488994
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021498.1_s317c1
- DNA Barcode:
- GTGGTTCAGACGCCCATGGGTATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A1 (6580)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488994
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6580 | SLC22A1 | solute carrier family 22 me... | NM_003057.3 | 99.8% | 99.6% | 480G>C;1222A>G |
| 2 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_005267103.2 | 93% | 84.4% | (many diffs) |
| 3 | human | 6580 | SLC22A1 | solute carrier family 22 me... | NM_153187.1 | 91.2% | 83% | (many diffs) |
| 4 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_006715552.2 | 87% | 86.8% | 480G>C;1222A>G;1383_1384ins213 |
| 5 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_005267102.5 | 80.8% | 78% | (many diffs) |
| 6 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_005267104.5 | 51% | 48.3% | (many diffs) |
| 7 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_005267105.5 | 51% | 48.3% | (many diffs) |
| 8 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_011536074.3 | 51% | 48.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1737
- ORF length:
- 1662
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgccc accgtggatg acattctgga gcaggttggg gagtctggct 121 ggttccagaa gcaagccttc ctcatcttat gcctgctgtc ggctgccttt gcgcccatct 181 gtgtgggcat cgtcttcctg ggtttcacac ctgaccacca ctgccagagt cctggggtgg 241 ctgagctgag ccagcgctgt ggctggagcc ctgcggagga gctgaactat acagtgccag 301 gcctggggcc cgcgggcgag gccttccttg gccagtgcag gcgctatgaa gtggactgga 361 accagagcgc cctcagctgt gtagaccccc tggctagcct ggccaccaac aggagccacc 421 tgccgctggg tccctgccag gatggctggg tgtatgacac gcccggctct tccatcgtca 481 ctgagttcaa cctggtgtgt gctgactcct ggaagctgga cctctttcag tcctgtttga 541 atgcgggctt cttctttggc tctctcggtg ttggctactt tgcagacagg tttggccgta 601 agctgtgtct cctgggaact gtgctggtca acgcggtgtc gggcgtgctc atggccttct 661 cgcccaacta catgtccatg ctgctcttcc gcctgctgca gggcctggtc agcaagggca 721 actggatggc tggctacacc ctaatcacag aatttgttgg ctcgggctcc agaagaacgg 781 tggcgatcat gtaccagatg gccttcacgg tggggctggt ggcgcttacc gggctggcct 841 acgccctgcc tcactggcgc tggctgcagc tggcagtctc cctgcccacc ttcctcttcc 901 tgctctacta ctggtgtgtg ccggagtccc ctcggtggct gttatcacaa aaaagaaaca 961 ctgaagcaat aaagataatg gaccacatcg ctcaaaagaa tgggaagttg cctcctgctg 1021 atttaaagat gctttccctc gaagaggatg tcaccgaaaa gctgagccct tcatttgcag 1081 acctgttccg cacgccgcgc ctgaggaagc gcaccttcat cctgatgtac ctgtggttca 1141 cggactctgt gctctatcag gggctcatcc tgcacatggg cgccaccagc gggaacctct 1201 acctggattt cctttactcc gctctggtcg aaatcccggg ggccttcata gccctcatca 1261 ccattgaccg cgtgggccgc atctacccca tggccgtgtc aaatttgttg gcgggggcag 1321 cctgcctcgt catgattttt atctcacctg acctgcactg gttaaacatc ataatcatgt 1381 gtgttGGCCG AATGGGAATC ACCATTGCAA TACAAATGAT CTGCCTGGTG AATGCTGAGC 1441 TGTACCCCAC ATTCGTCAGG AACCTCGGAG TGATGGTGTG TTCCTCCCTG TGTGACATAG 1501 GTGGGATAAT CACCCCCTTC ATAGTCTTCA GGCTGAGGGA GGTCTGGCAA GCCTTGCCCC 1561 TCATTTTGTT TGCGGTGTTG GGCCTGCTTG CCGCGGGAGT GACGCTACTT CTTCCAGAGA 1621 CCAAGGGGGT CGCTTTGCCA GAGACCATGA AGGACGCCGA GAACCTTGGG AGAAAAGCAA 1681 AGCCCAAAGA AAACACGATT TACCTTAAGG TCCAAACCTC AGAACCCTCG GGCACCTGAT 1741 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1801 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1861 GGCTTTATAT ATCTTGTGGA AAGGACGAGT GGTTCAGACG CCCATGGGTA TTACGCGTTA 1921 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt