Construct: ORF TRCN0000488996
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021453.1_s317c1
- DNA Barcode:
- TTTATAATACATGACCAGAGGCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ADSS1 (122622)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488996
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122622 | ADSS1 | adenylosuccinate synthase 1 | NM_152328.4 | 99.4% | 100% | 1371_1372insTAGAAGCT |
| 2 | human | 122622 | ADSS1 | adenylosuccinate synthase 1 | XM_006720026.3 | 98.2% | 98.6% | (many diffs) |
| 3 | human | 122622 | ADSS1 | adenylosuccinate synthase 1 | NM_199165.2 | 86.6% | 79.5% | (many diffs) |
| 4 | human | 122622 | ADSS1 | adenylosuccinate synthase 1 | XM_011536412.2 | 85.6% | 78.4% | (many diffs) |
| 5 | human | 122622 | ADSS1 | adenylosuccinate synthase 1 | NM_001320424.1 | 54.8% | 55.1% | 0_1ins615;756_757insTAGAAGCT |
| 6 | mouse | 11565 | Adssl1 | adenylosuccinate synthetase... | NM_007421.2 | 88.7% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1437
- ORF length:
- 1371
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatgtc ggggacccga gcctccaacg accggccccc cggcgcaggc ggcgtcaagc 121 gggggcggct gcagcaggag gcggcggcga ccggctcccg cgtgacggtg gtgctgggcg 181 cgcagtgggg ggacgagggc aaaggcaagg tggtggacct gctggccacg gacgccgaca 241 tcatcagccg ctgccagggg ggcaacaacg ccggccacac ggtggtggtg gatgggaaag 301 agtacgactt ccacctgctg cccagcggca tcatcaacac caaggccgtg tccttcattg 361 gcaacggggt ggtcatccac ttgccaggct tgtttgagga agcagagaag aatgaaaaga 421 aaggcctgaa ggactgggag aagaggctca tcatctctga cagagcccac cttgtgtttg 481 attttcacca ggctgtcgac ggacttcagg aagtgcagcg ccaggcacaa gaggggaaga 541 atataggcac caccaagaag ggaatcggac caacctactc ttccaaagct gcccggacag 601 gcctccgcat ctgcgacctc ctgtcagatt ttgatgagtt ttcctccaga ttcaagaacc 661 tggcccacca gcaccagtcg atgttcccca ccctggaaat agacattgaa ggccaactca 721 aaaggctcaa gggctttgct gagcggatca gacccatggt ccgagatggt gtttacttta 781 tgtatgaggc actccacggc ccccccaaga agatcctggt ggagggtgcc aacgccgccc 841 tcctcgacat tgacttcggg acctacccct ttgtgacttc atccaactgc accgtgggcg 901 gtgtgtgcac gggcctgggc atccccccgc agaacatagg tgacgtgtat ggcgtggtga 961 aagcctatac cacacgtgtg ggcatcgggg ccttccccac cgagcagatc aacgagattg 1021 gaggcctGCT GCAGACCCGC GGCCACGAGT GGGGAGTGAC CACAGGCAGG AAGAGGCGCT 1081 GCGGCTGGCT CGACCTGATG ATTCTAAGAT ATGCTCACAT GGTCAACGGA TTCACTGCGC 1141 TGGCCCTGAC GAAGCTGGAC ATCCTGGACG TACTGGGTGA GGTTAAAGTC GGTGTCTCAT 1201 ACAAGCTGAA CGGGAAAAGG ATTCCCTATT TCCCAGCTAA CCAGGAGATG CTTCAGAAGG 1261 TCGAAGTTGA GTATGAAACG CTGCCTGGGT GGAAAGCAGA CACCACAGGC GCCAGGAGGT 1321 GGGAGGACCT GCCCCCACAG GCCCAGAACT ACATCCGCTT TGTGGAGAAT CACGTGGGAG 1381 TCGCAGTCAA ATGGGTTGGT GTTGGCAAGT CAAGAGAGTC GATGATCCAG CTGTTTTAGA 1441 AGCTTGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1501 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1561 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTTATAATA CATGACCAGA GGCATACGCG 1621 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt