Construct: ORF TRCN0000489006
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020347.1_s317c1
- DNA Barcode:
- ACCAACCCCAATGGTCGTTTGGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PSKH1 (5681)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489006
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5681 | PSKH1 | protein serine kinase H1 | NM_006742.3 | 100% | 100% | |
| 2 | mouse | 244631 | Pskh1 | protein serine kinase H1 | NM_173432.2 | 88.5% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1344
- ORF length:
- 1272
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggctgt gggacaagca aggtccttcc cgagccaccc aaggatgtcc 121 agctggatct ggtcaagaag gtggagccct tcagtggcac taagagtgac gtgtacaagc 181 acttcatcac agaggtggac agtgttggcc ctgtcaaagc cgggttccca gcagcaagtc 241 agtatgcaca cccctgcccc ggtcccccga ctgctggcca cacggagcct ccctcagaac 301 caccacgcag ggccagggta gctaagtaca gggccaagtt tgacccacgt gttacagcta 361 agtatgacat caaggcccta attggccgag gcagcttcag ccgagtggta cgtgtagagc 421 accgggcaac ccggcagccg tatgccatca agatgattga gaccaagtac cgggaggggc 481 gggaggtgtg tgagtcggag ctgcgtgtgc tgcgtcgggt gcgtcatgcc aacatcatcc 541 agctggtgga ggtgttcgag acacaggagc gggtgtacat ggtgatggag ctggccactg 601 gtggagagct ctttgaccgc atcattgcca agggctcctt caccgagcgt gacgccacgc 661 gggtgctgca gatggtgctg gatggcgtcc ggtatctgca tgcactgggc atcacacacc 721 gagacctcaa acctgagaat ctgctctact accatccggg cactgactcc aagatcatca 781 tcaccgactt cggcctggcc agtgctcgca agaagggtga tgactgcttg atgaagacca 841 cctgtggcac gcctgagtac attgccccag aagtcctggt ccgcaagcca tacaccaact 901 cagtggacat gtgggcgctg ggcgtcattg cctacatcct actcagtggc accatgccgt 961 ttgaggatga caaccgtacc cggctgtacc ggcagatcct caggggcaag tacagttact 1021 ctggggagcc ctggcctagt gtgtccaacc tggccaagga cttcattgac cgcctgctga 1081 cagtggaccc tggagcccgt atgactgcac tgcaggcccT GAGGCACCCG TGGGTGGTGA 1141 GCATGGCTGC CTCTTCATCC ATGAAGAACC TGCACCGCTC CATATCCCAG AACCTCCTTA 1201 AACGTGCCTC CTCGCGCTGC CAGAGCACCA AATCTGCCCA GTCCACGCGT TCCAGCCGCT 1261 CCACACGCTC CAATAAGTCA CGCCGTGTGC GGGAACGGGA GCTGCGGGAG CTCAACCTGC 1321 GCTACCAGCA GCAATACAAT GGCTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCAACCC 1501 CAATGGTCGT TTGGTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1561 aagatt