Construct: ORF TRCN0000489011
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019204.1_s317c1
- DNA Barcode:
- CTAATCTTTTGTAGGGGAGGGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPFFR1 (64106)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489011
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64106 | NPFFR1 | neuropeptide FF receptor 1 | NM_022146.5 | 99.9% | 100% | 597G>T |
| 2 | mouse | 237362 | Npffr1 | neuropeptide FF receptor 1 | NM_001177511.1 | 86% | 86.5% | (many diffs) |
| 3 | mouse | 237362 | Npffr1 | neuropeptide FF receptor 1 | XM_011243444.2 | 62.5% | 63.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1365
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag ggggagccct cccagcctcc caacagcagt tggcccctaa 121 gtcagaatgg gactaacact gaggccaccc cggctacaaa cctcaccttc tcctcctact 181 atcagcacac ctcccctgtg gcggccatgt tcattgtggc ctatgcgctc atcttcctgc 241 tctgcatggt gggcaacacc ctggtctgtt tcatcgtgct caagaaccgg cacatgcata 301 ctgtcaccaa catgttcatc ctcaacctgg ctgtcagtga cctgctggtg ggcatcttct 361 gcatgcccac cacccttgtg gacaacctca tcactgggtg gcccttcgac aatgccacat 421 gcaagatgag cggcttggtg cagggcatgt ctgtgtcggc ttccgttttc acactggtgg 481 ccattgctgt ggaaaggttc cgctgcatcg tgcacccttt ccgcgagaag ctgaccctgc 541 ggaaggcgct cgtcaccatc gccgtcatct gggccctggc gctgctcatc atgtgtccct 601 cggccgtcac gctgaccgtc acccgtgagg agcaccactt catggtggac gcccgcaacc 661 gctcctaccc tctctactcc tgctgggagg cctggcccga gaagggcatg cgcagggtct 721 acaccactgt gctcttctcg cacatctacc tggcgccgct ggcgctcatc gtggtcatgt 781 acgcccgcat cgcgcgcaag ctctgccagg ccccgggccc ggcccccggg ggcgaggagg 841 ctgcggaccc gcgagcatcg cggcgcagag cgcgcgtggt gcacatgctg gtcatggtgg 901 cgctgttctt cacgctgtcc tggctgccgc tctgggcgct gctgctgctc atcgactacg 961 ggcagctcag cgcgccgcag ctgcacctgg tcaccgtcta cgccttcccc ttcgcgcact 1021 ggctggcctt cttcaacagc agcgccaacc ccatcatcta cggctacttc aacgagaact 1081 tccgccgcgg cttccaggcc gccttccgcg cccgcctctg cccgcgcccg tcggggagcc 1141 acaaggaggc ctacTCCGAG CGGCCCGGCG GGCTTCTGCA CAGGCGGGTC TTCGTGGTGG 1201 TGCGGCCCAG CGACTCCGGG CTGCCCTCTG AGTCGGGCCC TAGCAGTGGG GCCCCCAGGC 1261 CCGGCCGCCT CCCGCTGCGG AATGGGCGGG TGGCTCACCA CGGCTTGCCC AGGGAAGGGC 1321 CTGGCTGCTC CCACCTGCCC CTCACCATTC CAGCCTGGGA TATCGACCCA GCTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA CTAATCTTTT GTAGGGGAGG GGGTACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt