Construct: ORF TRCN0000489042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020659.1_s317c1
- DNA Barcode:
- TAACCAACGAGCTATTCGTATTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAPK15 (225689)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489042
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 225689 | MAPK15 | mitogen-activated protein k... | NM_139021.3 | 100% | 100% | |
| 2 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_011516925.2 | 93.5% | 91.7% | (many diffs) |
| 3 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_011516926.2 | 88.9% | 81.5% | (many diffs) |
| 4 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_011516927.2 | 88.9% | 79.7% | (many diffs) |
| 5 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_017013218.1 | 80.8% | 72.2% | (many diffs) |
| 6 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_011516928.2 | 51.8% | 50.2% | (many diffs) |
| 7 | human | 225689 | MAPK15 | mitogen-activated protein k... | XR_001745493.1 | 38.1% | (many diffs) | |
| 8 | human | 225689 | MAPK15 | mitogen-activated protein k... | XR_001745494.1 | 38.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 1731
- ORF length:
- 1632
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccggat ccgccgccat gtgcaccgta gtggaccctc 121 gcattgtccg gagataccta ctcaggcggc agctcgggca gggggcctat ggcattgtgt 181 ggaaggcagt ggaccggagg actggtgagg tcgtggccat caagaaaatc tttgatgctt 241 ttagggataa gacagatgcc cagagaacat tccgggaaat cacgctcctc caggagtttg 301 gggaccatcc caacatcatc agcctccttg acgtgatccg ggcagagaac gacagggaca 361 tttacctggt gtttgagttt atggacactg acctgaacgc agtcatccgg aagggcggcc 421 tgctgcagga cgtccacgtg cgctccatct tctaccagct cctgcgggcc acccggttcc 481 tccactcggg gcacgttgtg caccgggacc agaagccgtc caatgtgctc ctggatgcca 541 actgcacagt gaagctgtgt gactttggcc tggcccgctc cctgggcgac ctccccgagg 601 ggcctgagga ccaggccgtg acagagtacg tggccacacg ctggtaccga gcaccggagg 661 tgctgctctc ttcgcaccga tacacccttg gggtggacat gtggagtctg ggctgtatcc 721 tgggggagat gctgcggggg agacccctgt tccccggcac gtccaccctc caccagctgg 781 agctgatcct ggagaccatc ccaccgccat ctgaggagga cctcctggct ctcggctcag 841 gctgccgtgc ctctgtgctg caccagctgg ggtcccggcc acgacagacg ctggatgccc 901 tcctaccgcc agacacctcc ccagaggcct tggacctcct taggcgactc ctggtgttcg 961 ccccggacaa gcggttaagc gcgacccagg cactgcagca cccctacgtg cagaggttcc 1021 actgccccag cgacgagtgg gcacgagagg cagatgtgcg gccccgggca cacgaagggg 1081 tccagctctc tgtgcctgag taccgcagcc gcgtctatca gatgatcctg gagtgtggag 1141 gcagcagcgg cacctcgaga gagaagggcc cggagggtgt ctccccaagc caggcacacc 1201 tgcacaaacc cagagccgac cctcagctgc cttctaggac acctgtgcag ggtcccagac 1261 ccaggcccca gagcagccca ggccatgacc ctgccgagca cgagtccccc cgtgcagcca 1321 agaacgttcc caggcagaac tccgctcccc tgctccaaac tgctctccta gggaatgggg 1381 aaaggccccc tggggcgaag gaagcgcccc ccttgacact ctcgctggtg aagccaagcg 1441 ggaggGGAGC TGCGCCCTCC CTGACCTCCC AGGCTGCGGC TCAGGTGGCC AACCAGGCCC 1501 TGATCCGGGG TGACTGGAAC CGGGGCGGTG GGGTGAGGGT GGCCAGCGTA CAACAGGTCC 1561 CTCCCCGGCT TCCTCCGGAG GCCCGGCCCG GCCGGAGGAT GTTCAGCACC TCTGCCTTGC 1621 AGGGTGCCCA GGGGGGTGCC AGGGCTTTGC TTGGAGGCTA CTCCCAAGCC TACGGGACTG 1681 TCTGCCACTC GGCACTGGGC CACCTGCCCC TGCTGGAGGG GCACCATGTG TGAAAGCTTA 1741 AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1801 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1861 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAACCAACGA GCTATTCGTA 1921 TTTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt