Construct: ORF TRCN0000489076
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020391.1_s317c1
- DNA Barcode:
- TCTCCCTTAAGCAACTGGCTCAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKCI (5584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489076
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5584 | PRKCI | protein kinase C iota | NM_002740.6 | 98.4% | 98.4% | 1_27del |
| 2 | human | 5584 | PRKCI | protein kinase C iota | XM_017006860.1 | 79% | 75.8% | 0_1ins283;54_55ins86 |
| 3 | human | 5584 | PRKCI | protein kinase C iota | XR_001740206.1 | 34.7% | 1_192del;1367_1368ins88;1866_4722del | |
| 4 | mouse | 18759 | Prkci | protein kinase C, iota | NM_008857.3 | 89% | 96.8% | (many diffs) |
| 5 | mouse | 18759 | Prkci | protein kinase C, iota | XM_006535411.3 | 84.5% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1833
- ORF length:
- 1761
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcccac acggtcgcag gcggcggcag cggggaccat tcccaccagg 121 tccgggtgaa agcctactac cgcggggata tcatgataac acattttgaa ccttccatct 181 cctttgaggg cctttgcaat gaggttcgag acatgtgttc ttttgacaac gaacagctct 241 tcaccatgaa atggatagat gaggaaggag acccgtgtac agtatcatct cagttggagt 301 tagaagaagc ctttagactt tatgagctaa acaaggattc tgaactcttg attcatgtgt 361 tcccttgtgt accagaacgt cctgggatgc cttgtccagg agaagataaa tccatctacc 421 gtagaggtgc acgccgctgg agaaagcttt attgtgccaa tggccacact ttccaagcca 481 agcgtttcaa caggcgtgct cactgtgcca tctgcacaga ccgaatatgg ggacttggac 541 gccaaggata taagtgcatc aactgcaaac tcttggttca taagaagtgc cataaactcg 601 tcacaattga atgtgggcgg cattctttgc cacaggaacc agtgatgccc atggatcagt 661 catccatgca ttctgaccat gcacagacag taattccata taatccttca agtcatgaga 721 gtttggatca agttggtgaa gaaaaagagg caatgaacac cagggaaagt ggcaaagctt 781 catccagtct aggtcttcag gattttgatt tgctccgggt aataggaaga ggaagttatg 841 ccaaagtact gttggttcga ttaaaaaaaa cagatcgtat ttatgcaatg aaagttgtga 901 aaaaagagct tgttaatgat gatgaggata ttgattgggt acagacagag aagcatgtgt 961 ttgagcaggc atccaatcat cctttccttg ttgggctgca ttcttgcttt cagacagaaa 1021 gcagattgtt ctttgttata gagtatgtaa atggaggaga cctaatgttt catatgcagc 1081 gacaaagaaa acttcctgaa gaacatgcca gattttactc tgcagaaatc agtctagcat 1141 taaattatct tcatgagcga gggataattt atagagattt gaaactggac aatgtattac 1201 tggactctga aggccacatt aaactcactg actacggcat gtgtaaggaa ggattacggc 1261 caggagatac aaccagcact ttctgtggta ctcctaatta cattgctcct gaaattttaa 1321 gaggagaaga ttatggtttc agtgttgact ggtgggctct tggagtgctc atgtttgaga 1381 tgatggcagg aaggtctcca tttgatattg ttgggagctc cgataaccct gaccagaaca 1441 cagaggatta tctcttccaa gttattttGG AAAAACAAAT TCGCATACCA CGTTCTCTGT 1501 CTGTAAAAGC TGCAAGTGTT CTGAAGAGTT TTCTTAATAA GGACCCTAAG GAACGATTGG 1561 GTTGTCATCC TCAAACAGGA TTTGCTGATA TTCAGGGACA CCCGTTCTTC CGAAATGTTG 1621 ATTGGGATAT GATGGAGCAA AAACAGGTGG TACCTCCCTT TAAACCAAAT ATTTCTGGGG 1681 AATTTGGTTT GGACAACTTT GATTCTCAGT TTACTAATGA ACCTGTCCAG CTCACTCCAG 1741 ATGACGATGA CATTGTGAGG AAGATTGATC AGTCTGAATT TGAAGGTTTT GAGTATATCA 1801 ATCCTCTTTT GATGTCTGCA GAAGAATGTG TCTAGGACCC AGCTTTCTTG TACAAAGTGG 1861 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1921 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1981 ATCTCCCTTA AGCAACTGGC TCAAGACGCG TTAAGTCgac aatcaacctc tggattacaa 2041 aatttgtgaa agatt