Construct: ORF TRCN0000489080
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020221.1_s317c1
- DNA Barcode:
- GGCACTCATTCGATCTGATTAACT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DLG5 (9231)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489080
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_024448250.1 | 47% | 47% | 1_2280del |
| 2 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_006718056.3 | 42.6% | 42.6% | 1_2715del |
| 3 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_011540347.2 | 41.7% | 41.7% | 1_2817del |
| 4 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_017016915.1 | 41.7% | 41.7% | 1_2817del |
| 5 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_011540345.1 | 38.2% | 38.2% | 1_3270del |
| 6 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_011540344.2 | 37.2% | 37.2% | 1_3399del |
| 7 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_017016914.1 | 37.2% | 37.2% | 1_3405del |
| 8 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_017016913.1 | 36.9% | 36.9% | 1_3453del |
| 9 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_011540342.1 | 36.8% | 36.8% | 1_3465del |
| 10 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_011540341.3 | 36.2% | 36.2% | 1_3558del |
| 11 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_005270276.4 | 35.1% | 35.1% | 1_3723del |
| 12 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | NM_004747.4 | 35.1% | 35.1% | 1_3735del |
| 13 | human | 9231 | DLG5 | discs large MAGUK scaffold ... | XM_011540346.2 | 22.3% | 21.4% | (many diffs) |
| 14 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | XM_017316202.1 | 31.9% | 33.3% | (many diffs) |
| 15 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | XM_017316201.1 | 31.2% | 32.6% | (many diffs) |
| 16 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | NM_027726.1 | 31% | 32.4% | (many diffs) |
| 17 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | XM_017316200.1 | 30.8% | 32.2% | (many diffs) |
| 18 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | NM_001163513.1 | 30.6% | 32% | (many diffs) |
| 19 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | XM_006519560.2 | 30.5% | 31.9% | (many diffs) |
| 20 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | XM_017316199.1 | 30.5% | 31.9% | (many diffs) |
| 21 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | XM_017316197.1 | 30.5% | 31.8% | (many diffs) |
| 22 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | XM_017316198.1 | 30.4% | 31.9% | (many diffs) |
| 23 | mouse | 71228 | Dlg5 | discs, large homolog 5 (Dro... | XM_017316203.1 | 22.6% | 22.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2094
- ORF length:
- 2022
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagagcc acccatgggt ccaactcact gccctccagc gcccgcctgg 121 gttcttcgag taacttgcag ttcaaggcgg aacgcattaa aatcccatca acaccaagat 181 atccgcggag tgtcgtgggc tccgagagag gttcagtgtc acattctgaa tgcagcactc 241 ctccacagtc acccctgaac atcgacaccc tgtcctcttg tagccagtcc cagacctcag 301 cctccacatt gcccagaatc gctgtcaacc ccgcgtccct cggggagcgg agaaaggaca 361 ggccttatgt ggaggagcca cgccacgtga aggtgcagaa gggctcagag ccgctgggca 421 tctccatcgt gagtggagag aagggcggca tctacgtctc caaggtgacc gtggggagca 481 tcgctcacca ggctggcctc gagtatgggg atcagttact ggagttcaac ggcataaacc 541 tgcggagcgc cacggagcag caggcgcggc tcatcatcgg gcagcagtgt gataccatca 601 ccatcctggc ccagtacaac ccccacgtgc accagctcag cagccactcc cggtccagct 661 cacacctgga ccctgccggt acccactcca ctctccaggg cagtggcacc accaccccgg 721 agcatccatc tgtcatcgac ccactgatgg agcaggacga ggggcctagc acccccccag 781 ccaagcagag cagctccagg attgcgggag atgccaacaa gaagaccctg gagccacgcg 841 ttgtcttcat caaaaagtcc cagctggagc ttggggtgca cttgtgtggt gggaacctgc 901 atggggtgtt tgtggccgag gtggaggatg acagtcctgc caagggtcct gacggcctcg 961 tgccagggga cctcatcctg gagtatggca gcctggacgt gcggaacaag acagtggagg 1021 aagtctatgt ggagatgctg aagcccaggg atggcgtccg cctgaaggtg cagtaccgcc 1081 ctgaggagtt cacgaaggcc aagggcctgc ctggtgacag cttctacatc agggccctgt 1141 acgaccggct ggcagatgtg gagcaagagt tgagctttaa gaaggacgac atcctctacg 1201 tggatgacac cttaccccag ggcacgttcg ggtcctggat ggcttggcag ctggacgaga 1261 atgcccagaa gatccagcgc gggcagattc ccagcaaata tgtgatggac caagaattct 1321 ccaggaggct cagcatgtct gaagtcaaag atgacaatag cgccacaaag acgctgtcag 1381 cggctgcacg ccggtccttt tttcggagga aacacaagca caaacgcagc gggtccaagg 1441 acgggaaaga cctgctcgcc ttggatgcct tttccagtga ctccattcca ctctttgaag 1501 attcggtgag cctggcctat cagcgggtcc agaaggtgga ctgcaccgct ctgaggcctg 1561 tcctgattct ggggcctttg ctggacgtgg tgaaggagat gctggtgaat gaggctcctg 1621 gcaagttctg cagatgtccc cttgaggtga tgaaggcctc ccagcaggcc attgagcggg 1681 gtgtcaaaga ttgcctgttt gtcgactata agcggagaag cggccatttc gatgtgacca 1741 ctgtggcgtc aataaaggag atcacagaaa agaaccgaca ctgccTCCTG GACATTGCTC 1801 CGCACGCTAT TGAGCGGCTC CACCACATGC ACATCTACCC CATTGTCATC TTCATCCACT 1861 ACAAGAGCGC CAAGCACATC AAGGAGCAGA GAGACCCCAT CTACCTGAGG GACAAGGTGA 1921 CTCAGAGGCA TTCCAAAGAG CAGTTTGAGG CGGCGCAGAA GCTTGAGCAG GAGTACAGCA 1981 GGTACTTCAC AGGGGTCATC CAGGGAGGAG CCCTGTCAAG CATTTGCACT CAGATCTTGG 2041 CAATGGTCAA TCAAGAACAA AATAAAGTCC TGTGGATTCC AGCCTGCCCG CTCTGAGACC 2101 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2161 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2221 ATATATCTTG TGGAAAGGAC GAGGCACTCA TTCGATCTGA TTAACTACGC GTTAAGTCga 2281 caatcaacct ctggattaca aaatttgtga aagatt